Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4801 and GFF4802 are separated by 1 nucleotides GFF4802 and GFF4803 are separated by 190 nucleotides GFF4803 and GFF4804 are separated by 69 nucleotides GFF4804 and GFF4805 overlap by 10 nucleotides GFF4805 and GFF4806 are separated by 63 nucleotides GFF4806 and GFF4807 are separated by 4 nucleotides
GFF4801 - Alkaline phosphatase isozyme conversion protein precursor (EC 3.4.11.-), at 105,333 to 106,379
GFF4801
GFF4802 - FIG01045727: hypothetical protein, at 106,381 to 106,554
GFF4802
GFF4803 - hypothetical protein, at 106,745 to 106,891
GFF4803
GFF4804 - hypothetical protein, at 106,961 to 107,092
GFF4804
GFF4805 - FIG01046225: hypothetical protein, at 107,083 to 107,202
GFF4805
GFF4806 - FIG01045727: hypothetical protein, at 107,266 to 107,457
GFF4806
GFF4807 - CRISPR associated protein of unknown function, at 107,462 to 108,118
GFF4807
Position (kb)
107
108 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 106.253 kb on - strand, within GFF4801 at 106.321 kb on + strand at 106.322 kb on - strand at 106.322 kb on - strand at 106.322 kb on - strand at 106.322 kb on - strand at 106.381 kb on - strand at 106.399 kb on + strand, within GFF4802 at 106.555 kb on + strand at 106.555 kb on + strand at 106.555 kb on + strand at 106.675 kb on + strand at 106.675 kb on + strand at 106.677 kb on + strand at 106.678 kb on - strand at 107.157 kb on - strand, within GFF4805 at 107.163 kb on - strand, within GFF4805 at 107.212 kb on + strand at 107.212 kb on + strand at 107.213 kb on - strand at 107.324 kb on + strand, within GFF4806 at 107.400 kb on + strand, within GFF4806 at 108.066 kb on + strand at 108.127 kb on - strand at 108.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 106,253 - GFF4801 0.88 +1.4 106,321 + +0.7 106,322 - -0.6 106,322 - +0.5 106,322 - +1.2 106,322 - -0.7 106,381 - +0.3 106,399 + GFF4802 0.10 +0.1 106,555 + -1.3 106,555 + -0.6 106,555 + -0.4 106,675 + +6.6 106,675 + +0.1 106,677 + -1.1 106,678 - -1.6 107,157 - GFF4805 0.62 -0.2 107,163 - GFF4805 0.67 +1.1 107,212 + -0.2 107,212 + -1.4 107,213 - +0.3 107,324 + GFF4806 0.30 +1.5 107,400 + GFF4806 0.70 +0.7 108,066 + +0.8 108,127 - +0.2 108,127 - +0.1
Or see this region's nucleotide sequence