Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4271 and GFF4272 are separated by 346 nucleotides GFF4272 and GFF4273 are separated by 14 nucleotides
GFF4271 - Penicillin-binding protein AmpH, at 59,067 to 60,224
GFF4271
GFF4272 - SbmA protein, at 60,571 to 61,791
GFF4272
GFF4273 - Putative outer membrane lipoprotein, at 61,806 to 62,900
GFF4273
Position (kb) 60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 59.615 kb on + strand, within GFF4271 at 59.615 kb on + strand, within GFF4271 at 59.616 kb on - strand, within GFF4271 at 59.852 kb on - strand, within GFF4271 at 59.882 kb on - strand, within GFF4271 at 60.094 kb on + strand, within GFF4271 at 60.095 kb on - strand, within GFF4271 at 60.095 kb on - strand, within GFF4271 at 60.102 kb on - strand, within GFF4271 at 60.176 kb on + strand at 60.215 kb on + strand at 60.237 kb on + strand at 60.237 kb on + strand at 60.237 kb on + strand at 60.238 kb on - strand at 60.247 kb on - strand at 60.247 kb on - strand at 60.267 kb on - strand at 60.267 kb on - strand at 60.268 kb on + strand at 60.388 kb on - strand at 60.452 kb on + strand at 60.467 kb on - strand at 60.467 kb on - strand at 60.467 kb on - strand at 60.516 kb on + strand at 60.758 kb on - strand, within GFF4272 at 60.802 kb on + strand, within GFF4272 at 60.802 kb on + strand, within GFF4272 at 61.054 kb on + strand, within GFF4272 at 61.054 kb on + strand, within GFF4272 at 61.055 kb on - strand, within GFF4272 at 61.058 kb on + strand, within GFF4272 at 61.059 kb on - strand, within GFF4272 at 61.059 kb on - strand, within GFF4272 at 61.090 kb on + strand, within GFF4272 at 61.090 kb on + strand, within GFF4272 at 61.091 kb on - strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.092 kb on + strand, within GFF4272 at 61.108 kb on + strand, within GFF4272 at 61.152 kb on + strand, within GFF4272 at 61.153 kb on - strand, within GFF4272 at 61.406 kb on - strand, within GFF4272 at 61.492 kb on - strand, within GFF4272 at 61.496 kb on - strand, within GFF4272 at 61.516 kb on + strand, within GFF4272 at 61.517 kb on - strand, within GFF4272 at 61.517 kb on - strand, within GFF4272 at 61.517 kb on - strand, within GFF4272 at 61.518 kb on + strand, within GFF4272 at 61.522 kb on + strand, within GFF4272 at 61.522 kb on + strand, within GFF4272 at 61.522 kb on + strand, within GFF4272 at 61.523 kb on - strand, within GFF4272 at 61.523 kb on - strand, within GFF4272 at 61.546 kb on + strand, within GFF4272 at 61.547 kb on - strand, within GFF4272 at 61.719 kb on + strand at 61.728 kb on - strand at 61.785 kb on + strand at 61.868 kb on + strand at 61.872 kb on - strand at 61.927 kb on + strand, within GFF4273 at 61.997 kb on + strand, within GFF4273 at 62.030 kb on + strand, within GFF4273 at 62.103 kb on - strand, within GFF4273 at 62.205 kb on + strand, within GFF4273 at 62.208 kb on - strand, within GFF4273 at 62.246 kb on + strand, within GFF4273 at 62.268 kb on - strand, within GFF4273 at 62.273 kb on + strand, within GFF4273 at 62.273 kb on + strand, within GFF4273 at 62.309 kb on + strand, within GFF4273 at 62.401 kb on + strand, within GFF4273 at 62.401 kb on + strand, within GFF4273 at 62.737 kb on - strand, within GFF4273
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 59,615 + GFF4271 0.47 +1.1 59,615 + GFF4271 0.47 -1.2 59,616 - GFF4271 0.47 +1.1 59,852 - GFF4271 0.68 -0.0 59,882 - GFF4271 0.70 +1.1 60,094 + GFF4271 0.89 -0.5 60,095 - GFF4271 0.89 -1.2 60,095 - GFF4271 0.89 +1.1 60,102 - GFF4271 0.89 -0.3 60,176 + +0.1 60,215 + -0.6 60,237 + +0.7 60,237 + +0.3 60,237 + +0.2 60,238 - +0.6 60,247 - +0.6 60,247 - +0.1 60,267 - -0.4 60,267 - -2.8 60,268 + +7.0 60,388 - -0.4 60,452 + -1.0 60,467 - +2.4 60,467 - +0.2 60,467 - +0.5 60,516 + -0.3 60,758 - GFF4272 0.15 +0.4 60,802 + GFF4272 0.19 -1.0 60,802 + GFF4272 0.19 -1.0 61,054 + GFF4272 0.40 -1.0 61,054 + GFF4272 0.40 +0.6 61,055 - GFF4272 0.40 +0.3 61,058 + GFF4272 0.40 +0.3 61,059 - GFF4272 0.40 +0.3 61,059 - GFF4272 0.40 -0.9 61,090 + GFF4272 0.43 +3.5 61,090 + GFF4272 0.43 -0.5 61,091 - GFF4272 0.43 +0.4 61,092 + GFF4272 0.43 -0.4 61,092 + GFF4272 0.43 -0.4 61,092 + GFF4272 0.43 +0.1 61,108 + GFF4272 0.44 -0.1 61,152 + GFF4272 0.48 +0.3 61,153 - GFF4272 0.48 -1.3 61,406 - GFF4272 0.68 -0.0 61,492 - GFF4272 0.75 +7.9 61,496 - GFF4272 0.76 +0.7 61,516 + GFF4272 0.77 -0.4 61,517 - GFF4272 0.77 +2.6 61,517 - GFF4272 0.77 +0.6 61,517 - GFF4272 0.77 +0.0 61,518 + GFF4272 0.78 +1.8 61,522 + GFF4272 0.78 +0.5 61,522 + GFF4272 0.78 +1.2 61,522 + GFF4272 0.78 +0.3 61,523 - GFF4272 0.78 -1.1 61,523 - GFF4272 0.78 +1.0 61,546 + GFF4272 0.80 +0.5 61,547 - GFF4272 0.80 +1.0 61,719 + +0.3 61,728 - -0.6 61,785 + -0.8 61,868 + +1.3 61,872 - +0.3 61,927 + GFF4273 0.11 -0.0 61,997 + GFF4273 0.17 +0.4 62,030 + GFF4273 0.20 +0.3 62,103 - GFF4273 0.27 +0.5 62,205 + GFF4273 0.36 +0.6 62,208 - GFF4273 0.37 +0.8 62,246 + GFF4273 0.40 +5.0 62,268 - GFF4273 0.42 +1.3 62,273 + GFF4273 0.43 +0.1 62,273 + GFF4273 0.43 +0.4 62,309 + GFF4273 0.46 -0.2 62,401 + GFF4273 0.54 +0.3 62,401 + GFF4273 0.54 +0.2 62,737 - GFF4273 0.85 +0.2
Or see this region's nucleotide sequence