Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4100 and GFF4101 are separated by 239 nucleotides GFF4101 and GFF4102 are separated by 212 nucleotides GFF4102 and GFF4103 overlap by 30 nucleotides
GFF4100 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 58,007 to 60,451
GFF4100
GFF4101 - Phosphoheptose isomerase 1 (EC 5.3.1.-), at 60,691 to 61,269
GFF4101
GFF4102 - Predicted glutamine amidotransferase, at 61,482 to 62,249
GFF4102
GFF4103 - probable exported protein STY0357, at 62,220 to 62,960
GFF4103
Position (kb) 60
61
62 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 59.805 kb on + strand, within GFF4100 at 59.805 kb on + strand, within GFF4100 at 60.109 kb on - strand, within GFF4100 at 60.170 kb on - strand, within GFF4100 at 60.384 kb on - strand at 60.436 kb on + strand at 60.437 kb on - strand at 60.500 kb on - strand at 60.634 kb on + strand at 60.952 kb on - strand, within GFF4101 at 61.223 kb on + strand at 61.224 kb on - strand at 61.271 kb on + strand at 61.272 kb on - strand at 61.273 kb on + strand at 61.273 kb on + strand at 61.335 kb on - strand at 61.338 kb on + strand at 61.397 kb on - strand at 61.480 kb on - strand at 61.480 kb on - strand at 61.481 kb on + strand at 61.482 kb on - strand at 61.482 kb on - strand at 61.523 kb on + strand at 61.602 kb on + strand, within GFF4102 at 61.629 kb on - strand, within GFF4102 at 61.751 kb on + strand, within GFF4102 at 61.752 kb on - strand, within GFF4102 at 61.795 kb on - strand, within GFF4102 at 61.795 kb on - strand, within GFF4102 at 61.950 kb on - strand, within GFF4102 at 61.953 kb on + strand, within GFF4102 at 61.954 kb on - strand, within GFF4102 at 61.954 kb on - strand, within GFF4102 at 61.955 kb on + strand, within GFF4102 at 61.955 kb on + strand, within GFF4102 at 61.955 kb on + strand, within GFF4102 at 61.975 kb on + strand, within GFF4102 at 61.976 kb on - strand, within GFF4102 at 61.978 kb on + strand, within GFF4102 at 61.998 kb on - strand, within GFF4102 at 62.083 kb on + strand, within GFF4102 at 62.201 kb on + strand at 62.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 59,805 + GFF4100 0.74 -0.5 59,805 + GFF4100 0.74 -0.5 60,109 - GFF4100 0.86 +0.0 60,170 - GFF4100 0.88 -0.8 60,384 - -1.5 60,436 + +0.4 60,437 - -0.5 60,500 - +4.6 60,634 + +1.0 60,952 - GFF4101 0.45 +1.7 61,223 + -0.7 61,224 - -1.7 61,271 + -0.0 61,272 - -0.2 61,273 + -0.6 61,273 + +0.7 61,335 - +0.4 61,338 + +2.1 61,397 - +5.9 61,480 - +1.2 61,480 - +0.1 61,481 + -0.4 61,482 - +0.8 61,482 - +0.2 61,523 + -0.2 61,602 + GFF4102 0.16 -0.3 61,629 - GFF4102 0.19 +0.1 61,751 + GFF4102 0.35 +1.5 61,752 - GFF4102 0.35 -0.0 61,795 - GFF4102 0.41 +0.6 61,795 - GFF4102 0.41 +0.4 61,950 - GFF4102 0.61 -1.8 61,953 + GFF4102 0.61 +0.1 61,954 - GFF4102 0.61 -0.3 61,954 - GFF4102 0.61 +1.2 61,955 + GFF4102 0.62 +0.2 61,955 + GFF4102 0.62 -1.0 61,955 + GFF4102 0.62 -0.4 61,975 + GFF4102 0.64 -0.8 61,976 - GFF4102 0.64 -2.3 61,978 + GFF4102 0.65 -0.4 61,998 - GFF4102 0.67 -0.3 62,083 + GFF4102 0.78 +3.3 62,201 + +0.3 62,241 - -1.2
Or see this region's nucleotide sequence