Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4016 and GFF4017 overlap by 8 nucleotides GFF4017 and GFF4018 overlap by 8 nucleotides GFF4018 and GFF4019 are separated by 107 nucleotides GFF4019 and GFF4020 are separated by 46 nucleotides GFF4020 and GFF4021 are separated by 119 nucleotides
GFF4016 - Flagellar assembly protein FliH, at 284,944 to 285,651
GFF4016
GFF4017 - Flagellar motor switch protein FliG, at 285,644 to 286,639
GFF4017
GFF4018 - Flagellar M-ring protein FliF, at 286,632 to 288,227
GFF4018
GFF4019 - FIG00640941: hypothetical protein, at 288,335 to 288,484
GFF4019
GFF4020 - Flagellar hook-basal body complex protein FliE, at 288,531 to 288,845
GFF4020
GFF4021 - FIG01200701: possible membrane protein, at 288,965 to 289,318
GFF4021
Position (kb) 286
287
288
289 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9 at 285.646 kb on - strand at 285.646 kb on - strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.655 kb on + strand at 285.655 kb on + strand at 285.709 kb on + strand at 285.808 kb on + strand, within GFF4017 at 285.808 kb on + strand, within GFF4017 at 286.052 kb on + strand, within GFF4017 at 286.140 kb on - strand, within GFF4017 at 286.351 kb on + strand, within GFF4017 at 286.351 kb on + strand, within GFF4017 at 286.475 kb on + strand, within GFF4017 at 286.529 kb on + strand, within GFF4017 at 286.539 kb on - strand, within GFF4017 at 286.539 kb on - strand, within GFF4017 at 286.539 kb on - strand, within GFF4017 at 286.647 kb on - strand at 287.222 kb on + strand, within GFF4018 at 287.234 kb on + strand, within GFF4018 at 287.265 kb on - strand, within GFF4018 at 287.265 kb on - strand, within GFF4018 at 287.417 kb on + strand, within GFF4018 at 287.997 kb on - strand, within GFF4018 at 288.149 kb on + strand at 288.149 kb on + strand at 288.541 kb on + strand at 288.563 kb on + strand, within GFF4020 at 288.572 kb on - strand, within GFF4020 at 288.644 kb on - strand, within GFF4020 at 288.644 kb on - strand, within GFF4020 at 288.644 kb on - strand, within GFF4020 at 288.891 kb on + strand at 289.181 kb on - strand, within GFF4021
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 285,646 - +0.9 285,646 - -0.5 285,651 + +0.5 285,651 + +0.4 285,651 + +1.0 285,651 + +0.2 285,651 + +1.2 285,655 + +0.7 285,655 + +1.0 285,709 + -0.8 285,808 + GFF4017 0.16 -1.7 285,808 + GFF4017 0.16 -0.4 286,052 + GFF4017 0.41 +0.5 286,140 - GFF4017 0.50 +0.7 286,351 + GFF4017 0.71 +9.3 286,351 + GFF4017 0.71 +1.4 286,475 + GFF4017 0.83 +0.6 286,529 + GFF4017 0.89 +0.1 286,539 - GFF4017 0.90 +1.0 286,539 - GFF4017 0.90 +0.7 286,539 - GFF4017 0.90 +1.9 286,647 - -0.6 287,222 + GFF4018 0.37 +1.8 287,234 + GFF4018 0.38 +0.6 287,265 - GFF4018 0.40 +0.0 287,265 - GFF4018 0.40 +0.6 287,417 + GFF4018 0.49 +1.2 287,997 - GFF4018 0.86 +1.4 288,149 + +0.1 288,149 + +0.9 288,541 + +0.5 288,563 + GFF4020 0.10 +1.5 288,572 - GFF4020 0.13 +0.7 288,644 - GFF4020 0.36 -1.0 288,644 - GFF4020 0.36 +0.2 288,644 - GFF4020 0.36 +0.6 288,891 + -0.4 289,181 - GFF4021 0.61 +0.3
Or see this region's nucleotide sequence