Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4015 and GFF4016 overlap by 1 nucleotides GFF4016 and GFF4017 overlap by 8 nucleotides GFF4017 and GFF4018 overlap by 8 nucleotides
GFF4015 - Flagellum-specific ATP synthase FliI, at 283,574 to 284,944
GFF4015
GFF4016 - Flagellar assembly protein FliH, at 284,944 to 285,651
GFF4016
GFF4017 - Flagellar motor switch protein FliG, at 285,644 to 286,639
GFF4017
GFF4018 - Flagellar M-ring protein FliF, at 286,632 to 288,227
GFF4018
Position (kb) 285
286
287 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9 at 284.708 kb on + strand, within GFF4015 at 284.728 kb on + strand, within GFF4015 at 284.852 kb on + strand at 285.033 kb on + strand, within GFF4016 at 285.033 kb on + strand, within GFF4016 at 285.646 kb on - strand at 285.646 kb on - strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.651 kb on + strand at 285.655 kb on + strand at 285.655 kb on + strand at 285.709 kb on + strand at 285.808 kb on + strand, within GFF4017 at 285.808 kb on + strand, within GFF4017 at 286.052 kb on + strand, within GFF4017 at 286.140 kb on - strand, within GFF4017 at 286.351 kb on + strand, within GFF4017 at 286.351 kb on + strand, within GFF4017 at 286.475 kb on + strand, within GFF4017 at 286.529 kb on + strand, within GFF4017 at 286.539 kb on - strand, within GFF4017 at 286.539 kb on - strand, within GFF4017 at 286.539 kb on - strand, within GFF4017 at 286.647 kb on - strand at 287.222 kb on + strand, within GFF4018 at 287.234 kb on + strand, within GFF4018 at 287.265 kb on - strand, within GFF4018 at 287.265 kb on - strand, within GFF4018 at 287.417 kb on + strand, within GFF4018
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 284,708 + GFF4015 0.83 -0.5 284,728 + GFF4015 0.84 +0.8 284,852 + +4.5 285,033 + GFF4016 0.13 -0.4 285,033 + GFF4016 0.13 +0.4 285,646 - +0.9 285,646 - -0.5 285,651 + +0.5 285,651 + +0.4 285,651 + +1.0 285,651 + +0.2 285,651 + +1.2 285,655 + +0.7 285,655 + +1.0 285,709 + -0.8 285,808 + GFF4017 0.16 -1.7 285,808 + GFF4017 0.16 -0.4 286,052 + GFF4017 0.41 +0.5 286,140 - GFF4017 0.50 +0.7 286,351 + GFF4017 0.71 +9.3 286,351 + GFF4017 0.71 +1.4 286,475 + GFF4017 0.83 +0.6 286,529 + GFF4017 0.89 +0.1 286,539 - GFF4017 0.90 +1.0 286,539 - GFF4017 0.90 +0.7 286,539 - GFF4017 0.90 +1.9 286,647 - -0.6 287,222 + GFF4018 0.37 +1.8 287,234 + GFF4018 0.38 +0.6 287,265 - GFF4018 0.40 +0.0 287,265 - GFF4018 0.40 +0.6 287,417 + GFF4018 0.49 +1.2
Or see this region's nucleotide sequence