Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3782 and GFF3783 are separated by 4 nucleotides GFF3783 and GFF3784 overlap by 12 nucleotides GFF3784 and GFF3785 are separated by 329 nucleotides
GFF3782 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 50,869 to 52,170
GFF3782
GFF3783 - Putative DNA-binding protein in cluster with Type I restriction-modification system, at 52,175 to 52,564
GFF3783
GFF3784 - S-formylglutathione hydrolase (EC 3.1.2.12), at 52,553 to 53,410
GFF3784
GFF3785 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 53,740 to 54,408
GFF3785
Position (kb) 52
53
54 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 51.698 kb on - strand, within GFF3782 at 51.778 kb on - strand, within GFF3782 at 51.792 kb on + strand, within GFF3782 at 51.963 kb on - strand, within GFF3782 at 52.085 kb on - strand at 52.110 kb on + strand at 52.126 kb on + strand at 52.127 kb on - strand at 52.182 kb on - strand at 52.186 kb on + strand at 52.342 kb on + strand, within GFF3783 at 52.343 kb on - strand, within GFF3783 at 52.496 kb on - strand, within GFF3783 at 52.642 kb on + strand, within GFF3784 at 52.643 kb on - strand, within GFF3784 at 52.643 kb on - strand, within GFF3784 at 52.643 kb on - strand, within GFF3784 at 52.851 kb on + strand, within GFF3784 at 52.956 kb on - strand, within GFF3784 at 53.012 kb on - strand, within GFF3784 at 53.012 kb on - strand, within GFF3784 at 53.187 kb on - strand, within GFF3784 at 53.187 kb on - strand, within GFF3784 at 53.272 kb on - strand, within GFF3784 at 53.272 kb on - strand, within GFF3784 at 53.439 kb on + strand at 53.509 kb on + strand at 54.410 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 51,698 - GFF3782 0.64 -0.2 51,778 - GFF3782 0.70 -1.1 51,792 + GFF3782 0.71 +0.7 51,963 - GFF3782 0.84 +0.6 52,085 - -0.3 52,110 + +0.3 52,126 + +0.1 52,127 - +0.1 52,182 - +0.4 52,186 + +0.3 52,342 + GFF3783 0.43 -0.1 52,343 - GFF3783 0.43 -0.9 52,496 - GFF3783 0.82 -0.5 52,642 + GFF3784 0.10 -0.2 52,643 - GFF3784 0.10 +0.8 52,643 - GFF3784 0.10 -0.1 52,643 - GFF3784 0.10 +7.4 52,851 + GFF3784 0.35 +0.1 52,956 - GFF3784 0.47 +0.1 53,012 - GFF3784 0.53 +1.1 53,012 - GFF3784 0.53 +0.2 53,187 - GFF3784 0.74 -0.9 53,187 - GFF3784 0.74 -0.1 53,272 - GFF3784 0.84 +0.5 53,272 - GFF3784 0.84 +0.2 53,439 + -0.5 53,509 + -0.9 54,410 + +1.7
Or see this region's nucleotide sequence