Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3719 and GFF3720 are separated by 265 nucleotides GFF3720 and GFF3721 overlap by 1 nucleotides GFF3721 and GFF3722 are separated by 10 nucleotides GFF3722 and GFF3723 are separated by 94 nucleotides
GFF3719 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 23,202 to 25,109
GFF3719
GFF3720 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 25,375 to 25,926
GFF3720
GFF3721 - Glutathione-regulated potassium-efflux system protein KefB, at 25,926 to 27,731
GFF3721
GFF3722 - Putative cytoplasmic protein ,probably associated with Glutathione-regulated potassium-efflux, at 27,742 to 27,942
GFF3722
GFF3723 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 28,037 to 28,627
GFF3723
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 24.992 kb on + strand at 25.037 kb on + strand at 25.119 kb on + strand at 25.120 kb on - strand at 25.156 kb on + strand at 25.194 kb on - strand at 25.253 kb on + strand at 25.254 kb on - strand at 25.254 kb on - strand at 25.282 kb on + strand at 25.283 kb on - strand at 25.283 kb on - strand at 25.283 kb on - strand at 25.319 kb on + strand at 25.320 kb on - strand at 25.345 kb on - strand at 25.345 kb on - strand at 25.405 kb on + strand at 25.406 kb on - strand at 25.406 kb on - strand at 25.478 kb on + strand, within GFF3720 at 25.548 kb on - strand, within GFF3720 at 25.586 kb on - strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.593 kb on + strand, within GFF3720 at 25.593 kb on + strand, within GFF3720 at 25.594 kb on - strand, within GFF3720 at 25.685 kb on - strand, within GFF3720 at 25.732 kb on + strand, within GFF3720 at 25.824 kb on + strand, within GFF3720 at 25.824 kb on + strand, within GFF3720 at 26.023 kb on + strand at 26.133 kb on - strand, within GFF3721 at 26.536 kb on + strand, within GFF3721 at 26.750 kb on + strand, within GFF3721 at 26.763 kb on - strand, within GFF3721 at 26.763 kb on - strand, within GFF3721 at 26.783 kb on - strand, within GFF3721 at 26.788 kb on - strand, within GFF3721 at 26.798 kb on - strand, within GFF3721 at 26.798 kb on - strand, within GFF3721 at 26.798 kb on - strand, within GFF3721 at 26.823 kb on - strand, within GFF3721 at 26.871 kb on + strand, within GFF3721 at 26.871 kb on + strand, within GFF3721 at 26.872 kb on - strand, within GFF3721 at 26.879 kb on - strand, within GFF3721 at 26.995 kb on + strand, within GFF3721 at 26.996 kb on - strand, within GFF3721 at 26.998 kb on + strand, within GFF3721 at 27.056 kb on + strand, within GFF3721 at 27.056 kb on + strand, within GFF3721 at 27.057 kb on - strand, within GFF3721 at 27.122 kb on + strand, within GFF3721 at 27.122 kb on + strand, within GFF3721 at 27.123 kb on - strand, within GFF3721 at 27.179 kb on - strand, within GFF3721 at 27.255 kb on + strand, within GFF3721 at 27.256 kb on - strand, within GFF3721 at 27.257 kb on + strand, within GFF3721 at 27.257 kb on + strand, within GFF3721 at 27.258 kb on - strand, within GFF3721 at 27.264 kb on + strand, within GFF3721 at 27.278 kb on + strand, within GFF3721 at 27.405 kb on - strand, within GFF3721 at 27.445 kb on + strand, within GFF3721 at 27.445 kb on + strand, within GFF3721 at 27.445 kb on + strand, within GFF3721 at 27.446 kb on - strand, within GFF3721 at 27.509 kb on + strand, within GFF3721 at 27.527 kb on - strand, within GFF3721 at 27.527 kb on - strand, within GFF3721 at 27.530 kb on + strand, within GFF3721 at 27.530 kb on + strand, within GFF3721 at 27.531 kb on - strand, within GFF3721 at 27.582 kb on - strand at 27.615 kb on - strand at 27.625 kb on + strand at 27.850 kb on - strand, within GFF3722 at 27.969 kb on - strand at 28.034 kb on - strand at 28.044 kb on + strand at 28.080 kb on + strand at 28.080 kb on + strand at 28.277 kb on - strand, within GFF3723 at 28.437 kb on + strand, within GFF3723 at 28.662 kb on + strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.689 kb on + strand at 28.689 kb on + strand at 28.690 kb on - strand at 28.690 kb on - strand at 28.693 kb on + strand at 28.693 kb on + strand at 28.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 24,992 + +0.3 25,037 + -1.6 25,119 + -0.3 25,120 - -0.1 25,156 + -0.2 25,194 - -0.1 25,253 + -0.3 25,254 - -0.7 25,254 - -0.7 25,282 + -0.9 25,283 - -1.4 25,283 - +0.1 25,283 - -0.5 25,319 + -0.8 25,320 - -0.5 25,345 - +0.1 25,345 - +1.1 25,405 + -0.3 25,406 - +0.6 25,406 - +0.3 25,478 + GFF3720 0.19 +0.1 25,548 - GFF3720 0.31 +1.1 25,586 - GFF3720 0.38 +0.2 25,591 + GFF3720 0.39 +0.6 25,591 + GFF3720 0.39 +0.1 25,591 + GFF3720 0.39 -0.3 25,591 + GFF3720 0.39 -0.2 25,591 + GFF3720 0.39 -0.2 25,591 + GFF3720 0.39 +3.1 25,591 + GFF3720 0.39 +0.1 25,591 + GFF3720 0.39 +0.7 25,592 - GFF3720 0.39 +1.3 25,592 - GFF3720 0.39 -0.7 25,592 - GFF3720 0.39 +0.3 25,592 - GFF3720 0.39 -0.5 25,592 - GFF3720 0.39 -0.3 25,592 - GFF3720 0.39 +0.4 25,592 - GFF3720 0.39 +0.6 25,592 - GFF3720 0.39 +0.2 25,593 + GFF3720 0.39 +0.9 25,593 + GFF3720 0.39 +0.0 25,594 - GFF3720 0.40 +0.8 25,685 - GFF3720 0.56 +0.2 25,732 + GFF3720 0.65 +0.3 25,824 + GFF3720 0.81 +0.7 25,824 + GFF3720 0.81 +0.7 26,023 + -0.6 26,133 - GFF3721 0.11 +0.7 26,536 + GFF3721 0.34 +0.3 26,750 + GFF3721 0.46 -0.2 26,763 - GFF3721 0.46 +0.0 26,763 - GFF3721 0.46 +0.3 26,783 - GFF3721 0.47 +0.7 26,788 - GFF3721 0.48 +0.5 26,798 - GFF3721 0.48 -1.4 26,798 - GFF3721 0.48 +6.1 26,798 - GFF3721 0.48 -0.9 26,823 - GFF3721 0.50 -0.2 26,871 + GFF3721 0.52 +1.0 26,871 + GFF3721 0.52 +0.5 26,872 - GFF3721 0.52 +0.3 26,879 - GFF3721 0.53 +2.0 26,995 + GFF3721 0.59 -0.2 26,996 - GFF3721 0.59 -0.0 26,998 + GFF3721 0.59 -0.3 27,056 + GFF3721 0.63 -0.3 27,056 + GFF3721 0.63 -0.1 27,057 - GFF3721 0.63 -0.4 27,122 + GFF3721 0.66 -0.2 27,122 + GFF3721 0.66 +0.5 27,123 - GFF3721 0.66 -0.4 27,179 - GFF3721 0.69 +0.5 27,255 + GFF3721 0.74 +0.0 27,256 - GFF3721 0.74 +0.5 27,257 + GFF3721 0.74 -0.4 27,257 + GFF3721 0.74 +2.0 27,258 - GFF3721 0.74 -0.0 27,264 + GFF3721 0.74 +1.0 27,278 + GFF3721 0.75 +0.5 27,405 - GFF3721 0.82 -0.5 27,445 + GFF3721 0.84 -0.9 27,445 + GFF3721 0.84 +0.5 27,445 + GFF3721 0.84 -0.6 27,446 - GFF3721 0.84 +0.3 27,509 + GFF3721 0.88 -0.4 27,527 - GFF3721 0.89 +0.2 27,527 - GFF3721 0.89 -0.6 27,530 + GFF3721 0.89 -0.7 27,530 + GFF3721 0.89 -0.5 27,531 - GFF3721 0.89 +0.1 27,582 - -0.9 27,615 - +0.8 27,625 + +0.1 27,850 - GFF3722 0.54 -0.6 27,969 - -2.1 28,034 - -0.8 28,044 + +1.2 28,080 + +1.8 28,080 + -1.1 28,277 - GFF3723 0.41 -0.5 28,437 + GFF3723 0.68 +2.0 28,662 + +1.2 28,686 - +1.5 28,686 - -0.3 28,686 - -0.2 28,686 - +0.3 28,686 - +1.4 28,686 - +0.1 28,686 - +0.2 28,689 + -1.2 28,689 + +0.9 28,690 - +0.6 28,690 - +0.5 28,693 + -1.2 28,693 + +0.1 28,694 - -0.3
Or see this region's nucleotide sequence