Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3536 and GFF3537 are separated by 155 nucleotides GFF3537 and GFF3538 are separated by 176 nucleotides GFF3538 and GFF3539 are separated by 46 nucleotides
GFF3536 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 287,451 to 288,497
GFF3536
GFF3537 - FIG137360: hypothetical protein, at 288,653 to 289,486
GFF3537
GFF3538 - hypothetical protein, at 289,663 to 289,776
GFF3538
GFF3539 - Phosphoenolpyruvate synthase (EC 2.7.9.2), at 289,823 to 292,201
GFF3539
Position (kb) 288
289
290 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9
10 at 287.658 kb on + strand, within GFF3536 at 287.659 kb on - strand, within GFF3536 at 287.664 kb on + strand, within GFF3536 at 287.840 kb on - strand, within GFF3536 at 287.844 kb on - strand, within GFF3536 at 287.879 kb on + strand, within GFF3536 at 287.881 kb on - strand, within GFF3536 at 288.063 kb on - strand, within GFF3536 at 288.139 kb on + strand, within GFF3536 at 288.647 kb on - strand at 288.671 kb on - strand at 288.881 kb on - strand, within GFF3537 at 288.887 kb on + strand, within GFF3537 at 288.919 kb on + strand, within GFF3537 at 288.950 kb on - strand, within GFF3537 at 289.110 kb on + strand, within GFF3537 at 289.454 kb on - strand at 289.756 kb on + strand, within GFF3538 at 289.770 kb on + strand at 289.778 kb on + strand at 290.054 kb on - strand at 290.267 kb on - strand, within GFF3539 at 290.363 kb on + strand, within GFF3539
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 287,658 + GFF3536 0.20 -0.7 287,659 - GFF3536 0.20 +0.6 287,664 + GFF3536 0.20 -0.1 287,840 - GFF3536 0.37 -0.9 287,844 - GFF3536 0.38 -0.3 287,879 + GFF3536 0.41 -0.4 287,881 - GFF3536 0.41 +0.6 288,063 - GFF3536 0.58 +0.1 288,139 + GFF3536 0.66 -0.5 288,647 - -0.1 288,671 - +9.9 288,881 - GFF3537 0.27 +0.5 288,887 + GFF3537 0.28 +0.0 288,919 + GFF3537 0.32 +0.5 288,950 - GFF3537 0.36 +0.1 289,110 + GFF3537 0.55 +0.6 289,454 - -0.7 289,756 + GFF3538 0.82 +0.3 289,770 + -0.9 289,778 + -0.0 290,054 - +0.9 290,267 - GFF3539 0.19 +1.4 290,363 + GFF3539 0.23 -0.5
Or see this region's nucleotide sequence