Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF351 and GFF352 overlap by 36 nucleotides GFF352 and GFF353 are separated by 89 nucleotides GFF353 and GFF354 are separated by 2 nucleotides GFF354 and GFF355 are separated by 2 nucleotides
GFF351 - Xanthine permease, at 70,435 to 71,826
GFF351
GFF352 - FIG01048298: hypothetical protein, at 71,791 to 71,964
GFF352
GFF353 - Sodium/glutamate symport protein, at 72,054 to 73,259
GFF353
GFF354 - putative cytoplasmic protein, at 73,262 to 74,113
GFF354
GFF355 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 74,116 to 76,197
GFF355
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 71.179 kb on - strand, within GFF351 at 71.511 kb on + strand, within GFF351 at 71.533 kb on + strand, within GFF351 at 71.677 kb on + strand, within GFF351 at 71.762 kb on - strand at 71.862 kb on - strand, within GFF352 at 72.040 kb on + strand at 72.041 kb on - strand at 72.041 kb on - strand at 72.052 kb on - strand at 72.152 kb on + strand at 72.182 kb on - strand, within GFF353 at 72.182 kb on - strand, within GFF353 at 72.243 kb on - strand, within GFF353 at 72.364 kb on + strand, within GFF353 at 72.365 kb on - strand, within GFF353 at 72.450 kb on - strand, within GFF353 at 72.597 kb on + strand, within GFF353 at 72.703 kb on + strand, within GFF353 at 72.729 kb on - strand, within GFF353 at 72.764 kb on - strand, within GFF353 at 72.809 kb on - strand, within GFF353 at 72.809 kb on - strand, within GFF353 at 72.988 kb on + strand, within GFF353 at 73.011 kb on + strand, within GFF353 at 73.118 kb on + strand, within GFF353 at 73.122 kb on - strand, within GFF353 at 73.158 kb on - strand at 73.440 kb on - strand, within GFF354 at 73.490 kb on + strand, within GFF354 at 73.490 kb on + strand, within GFF354 at 73.593 kb on - strand, within GFF354 at 73.814 kb on + strand, within GFF354 at 73.827 kb on + strand, within GFF354 at 73.891 kb on - strand, within GFF354
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 71,179 - GFF351 0.53 +0.9 71,511 + GFF351 0.77 +0.5 71,533 + GFF351 0.79 +0.2 71,677 + GFF351 0.89 +0.9 71,762 - +2.0 71,862 - GFF352 0.41 -0.9 72,040 + +0.4 72,041 - -0.1 72,041 - +0.4 72,052 - +2.3 72,152 + -0.7 72,182 - GFF353 0.11 -0.7 72,182 - GFF353 0.11 +0.1 72,243 - GFF353 0.16 -2.4 72,364 + GFF353 0.26 +0.4 72,365 - GFF353 0.26 +1.7 72,450 - GFF353 0.33 +0.8 72,597 + GFF353 0.45 -0.0 72,703 + GFF353 0.54 +8.0 72,729 - GFF353 0.56 +0.5 72,764 - GFF353 0.59 -0.5 72,809 - GFF353 0.63 -0.0 72,809 - GFF353 0.63 -1.4 72,988 + GFF353 0.77 +0.2 73,011 + GFF353 0.79 -0.3 73,118 + GFF353 0.88 +0.0 73,122 - GFF353 0.89 -0.0 73,158 - -0.5 73,440 - GFF354 0.21 +1.0 73,490 + GFF354 0.27 -0.2 73,490 + GFF354 0.27 +0.2 73,593 - GFF354 0.39 +0.4 73,814 + GFF354 0.65 +0.3 73,827 + GFF354 0.66 +2.9 73,891 - GFF354 0.74 +0.3
Or see this region's nucleotide sequence