Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF33 and GFF34 are separated by 62 nucleotides GFF34 and GFF35 are separated by 55 nucleotides GFF35 and GFF36 are separated by 75 nucleotides
GFF33 - tRNA(Ile)-lysidine synthetase (EC 6.3.4.19), at 9,126 to 10,418
GFF33
GFF34 - FIG01280259: hypothetical protein, at 10,481 to 10,870
GFF34
GFF35 - Lysine decarboxylase 2, constitutive (EC 4.1.1.18), at 10,926 to 13,067
GFF35
GFF36 - Chitinase (EC 3.2.1.14), at 13,143 to 14,906
GFF36
Position (kb) 10
11
12
13
14 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8 at 10.430 kb on - strand at 10.432 kb on - strand at 10.454 kb on - strand at 10.454 kb on - strand at 10.482 kb on + strand at 10.483 kb on - strand at 10.576 kb on + strand, within GFF34 at 10.710 kb on + strand, within GFF34 at 10.722 kb on - strand, within GFF34 at 10.860 kb on - strand at 11.348 kb on - strand, within GFF35 at 11.442 kb on - strand, within GFF35 at 11.475 kb on - strand, within GFF35 at 11.600 kb on + strand, within GFF35 at 11.618 kb on + strand, within GFF35 at 11.618 kb on + strand, within GFF35 at 11.619 kb on - strand, within GFF35 at 11.619 kb on - strand, within GFF35 at 11.620 kb on - strand, within GFF35 at 11.889 kb on - strand, within GFF35 at 11.931 kb on - strand, within GFF35 at 11.931 kb on - strand, within GFF35 at 12.062 kb on - strand, within GFF35 at 12.123 kb on - strand, within GFF35 at 12.145 kb on + strand, within GFF35 at 12.201 kb on + strand, within GFF35 at 12.342 kb on - strand, within GFF35 at 12.342 kb on - strand, within GFF35 at 12.355 kb on - strand, within GFF35 at 12.359 kb on + strand, within GFF35 at 12.379 kb on + strand, within GFF35 at 12.379 kb on + strand, within GFF35 at 12.379 kb on + strand, within GFF35 at 12.383 kb on + strand, within GFF35 at 12.396 kb on - strand, within GFF35 at 12.425 kb on + strand, within GFF35 at 12.425 kb on + strand, within GFF35 at 12.426 kb on - strand, within GFF35 at 12.426 kb on - strand, within GFF35 at 12.426 kb on - strand, within GFF35 at 12.427 kb on + strand, within GFF35 at 12.427 kb on + strand, within GFF35 at 12.428 kb on - strand, within GFF35 at 12.428 kb on - strand, within GFF35 at 12.634 kb on + strand, within GFF35 at 12.923 kb on + strand at 13.025 kb on + strand at 13.026 kb on - strand at 13.099 kb on - strand at 13.136 kb on + strand at 13.144 kb on + strand at 13.192 kb on + strand at 13.211 kb on + strand at 13.246 kb on + strand at 13.247 kb on - strand at 13.326 kb on - strand, within GFF36 at 13.424 kb on - strand, within GFF36 at 13.514 kb on + strand, within GFF36 at 13.517 kb on - strand, within GFF36 at 13.517 kb on - strand, within GFF36 at 13.517 kb on - strand, within GFF36 at 13.708 kb on + strand, within GFF36 at 13.709 kb on - strand, within GFF36 at 13.709 kb on - strand, within GFF36 at 13.709 kb on - strand, within GFF36 at 13.743 kb on - strand, within GFF36 at 13.754 kb on - strand, within GFF36 at 13.774 kb on - strand, within GFF36 at 13.927 kb on + strand, within GFF36 at 14.040 kb on + strand, within GFF36
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 10,430 - +0.7 10,432 - -1.0 10,454 - +1.1 10,454 - -0.6 10,482 + -0.0 10,483 - +1.2 10,576 + GFF34 0.24 -0.2 10,710 + GFF34 0.59 +0.2 10,722 - GFF34 0.62 -1.3 10,860 - +1.4 11,348 - GFF35 0.20 +1.0 11,442 - GFF35 0.24 +0.7 11,475 - GFF35 0.26 +0.8 11,600 + GFF35 0.31 -1.2 11,618 + GFF35 0.32 +2.1 11,618 + GFF35 0.32 -0.2 11,619 - GFF35 0.32 +2.3 11,619 - GFF35 0.32 +0.8 11,620 - GFF35 0.32 +0.6 11,889 - GFF35 0.45 +0.4 11,931 - GFF35 0.47 +1.2 11,931 - GFF35 0.47 +0.1 12,062 - GFF35 0.53 +0.4 12,123 - GFF35 0.56 +0.5 12,145 + GFF35 0.57 +1.8 12,201 + GFF35 0.60 -0.8 12,342 - GFF35 0.66 +0.1 12,342 - GFF35 0.66 -0.1 12,355 - GFF35 0.67 -1.2 12,359 + GFF35 0.67 -0.8 12,379 + GFF35 0.68 +0.6 12,379 + GFF35 0.68 +1.5 12,379 + GFF35 0.68 +0.8 12,383 + GFF35 0.68 +8.5 12,396 - GFF35 0.69 +0.2 12,425 + GFF35 0.70 -0.3 12,425 + GFF35 0.70 -0.1 12,426 - GFF35 0.70 -0.4 12,426 - GFF35 0.70 -0.2 12,426 - GFF35 0.70 +1.3 12,427 + GFF35 0.70 +0.0 12,427 + GFF35 0.70 -1.3 12,428 - GFF35 0.70 +0.5 12,428 - GFF35 0.70 -1.1 12,634 + GFF35 0.80 +0.4 12,923 + -0.2 13,025 + +0.2 13,026 - -0.9 13,099 - -0.3 13,136 + -1.1 13,144 + +1.8 13,192 + -0.1 13,211 + -0.3 13,246 + +0.4 13,247 - -0.2 13,326 - GFF36 0.10 -1.1 13,424 - GFF36 0.16 +1.1 13,514 + GFF36 0.21 +1.0 13,517 - GFF36 0.21 +1.5 13,517 - GFF36 0.21 +1.4 13,517 - GFF36 0.21 -0.3 13,708 + GFF36 0.32 -0.3 13,709 - GFF36 0.32 -0.6 13,709 - GFF36 0.32 +0.1 13,709 - GFF36 0.32 +1.4 13,743 - GFF36 0.34 +0.2 13,754 - GFF36 0.35 +0.6 13,774 - GFF36 0.36 +0.0 13,927 + GFF36 0.44 -0.4 14,040 + GFF36 0.51 +0.0
Or see this region's nucleotide sequence