Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3496 and GFF3497 are separated by 335 nucleotides GFF3497 and GFF3498 are separated by 74 nucleotides GFF3498 and GFF3499 are separated by 93 nucleotides GFF3499 and GFF3500 overlap by 48 nucleotides
GFF3496 - Succinylglutamate desuccinylase (EC 3.5.1.96), at 249,306 to 250,274
GFF3496
GFF3497 - Periplasmic protein related to spheroblast formation, at 250,610 to 251,095
GFF3497
GFF3498 - Excinuclease cho (excinuclease ABC alternative C subunit), at 251,170 to 252,078
GFF3498
GFF3499 - NAD synthetase (EC 6.3.1.5), at 252,172 to 252,999
GFF3499
GFF3500 - hypothetical protein, at 252,952 to 253,086
GFF3500
Position (kb) 251
252
253 Strain fitness (log2 ratio) -1
0
1 at 250.276 kb on - strand at 250.305 kb on - strand at 250.629 kb on + strand at 250.730 kb on - strand, within GFF3497 at 250.813 kb on + strand, within GFF3497 at 250.967 kb on - strand, within GFF3497 at 251.023 kb on + strand, within GFF3497 at 251.210 kb on + strand at 251.224 kb on + strand at 251.244 kb on + strand at 251.413 kb on + strand, within GFF3498 at 251.935 kb on + strand, within GFF3498 at 251.936 kb on - strand, within GFF3498 at 251.937 kb on + strand, within GFF3498 at 251.937 kb on + strand, within GFF3498 at 252.085 kb on + strand at 252.168 kb on - strand at 252.168 kb on - strand at 252.168 kb on - strand at 252.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 250,276 - +1.3 250,305 - +0.7 250,629 + +1.1 250,730 - GFF3497 0.25 -0.1 250,813 + GFF3497 0.42 -0.2 250,967 - GFF3497 0.73 +1.3 251,023 + GFF3497 0.85 -1.4 251,210 + +1.0 251,224 + +0.2 251,244 + +0.6 251,413 + GFF3498 0.27 +1.1 251,935 + GFF3498 0.84 -1.0 251,936 - GFF3498 0.84 -0.2 251,937 + GFF3498 0.84 -0.6 251,937 + GFF3498 0.84 -0.2 252,085 + -1.0 252,168 - -0.0 252,168 - +0.5 252,168 - -0.1 252,173 + +0.9
Or see this region's nucleotide sequence