Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3445 and GFF3446 are separated by 193 nucleotides GFF3446 and GFF3648 are separated by 8 nucleotides GFF3648 and GFF3447 are separated by 108 nucleotides GFF3447 and GFF3448 are separated by 27 nucleotides GFF3448 and GFF3449 are separated by 90 nucleotides
GFF3445 - Putative inner membrane protein, at 206,042 to 207,175
GFF3445
GFF3446 - Ferredoxin, at 207,369 to 207,695
GFF3446
GFF3648 - tRNA-Arg-TCT, at 207,704 to 207,780
GFF3648
GFF3447 - Candidate zinc-binding lipoprotein ZinT, at 207,889 to 208,539
GFF3447
GFF3448 - 'Streptomycin 3''-O-adenylyltransferase (EC 2.7.7.47) @ Spectinomycin 9-O-adenylyltransferase' transl_table=11, at 208,567 to 209,436
GFF3448
GFF3449 - Putative arylsulfatase regulatory protein, at 209,527 to 210,174
GFF3449
Position (kb) 207
208
209 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 206.959 kb on + strand, within GFF3445 at 206.989 kb on + strand, within GFF3445 at 206.990 kb on - strand, within GFF3445 at 207.043 kb on + strand, within GFF3445 at 207.044 kb on - strand, within GFF3445 at 207.046 kb on - strand, within GFF3445 at 207.056 kb on - strand, within GFF3445 at 207.079 kb on - strand at 207.205 kb on + strand at 207.212 kb on + strand at 207.213 kb on - strand at 207.213 kb on - strand at 207.335 kb on + strand at 207.340 kb on - strand at 207.340 kb on - strand at 207.491 kb on - strand, within GFF3446 at 207.533 kb on + strand, within GFF3446 at 207.740 kb on + strand, within GFF3648 at 208.033 kb on - strand, within GFF3447 at 208.069 kb on - strand, within GFF3447 at 208.190 kb on - strand, within GFF3447 at 208.232 kb on + strand, within GFF3447 at 208.342 kb on - strand, within GFF3447 at 208.479 kb on + strand at 208.483 kb on + strand at 208.484 kb on - strand at 208.537 kb on + strand at 208.538 kb on - strand at 208.538 kb on - strand at 208.538 kb on - strand at 208.619 kb on + strand at 208.746 kb on - strand, within GFF3448 at 209.045 kb on + strand, within GFF3448 at 209.073 kb on + strand, within GFF3448 at 209.198 kb on - strand, within GFF3448 at 209.198 kb on - strand, within GFF3448 at 209.230 kb on + strand, within GFF3448 at 209.233 kb on - strand, within GFF3448 at 209.306 kb on - strand, within GFF3448 at 209.338 kb on + strand, within GFF3448 at 209.339 kb on - strand, within GFF3448 at 209.434 kb on + strand at 209.434 kb on + strand at 209.435 kb on - strand at 209.435 kb on - strand at 209.484 kb on - strand at 209.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 206,959 + GFF3445 0.81 +5.8 206,989 + GFF3445 0.84 -0.0 206,990 - GFF3445 0.84 +0.5 207,043 + GFF3445 0.88 -0.1 207,044 - GFF3445 0.88 +0.6 207,046 - GFF3445 0.89 +0.3 207,056 - GFF3445 0.89 -0.9 207,079 - -0.0 207,205 + -0.1 207,212 + +1.0 207,213 - +1.8 207,213 - +0.0 207,335 + -0.6 207,340 - +0.2 207,340 - +0.3 207,491 - GFF3446 0.37 +0.4 207,533 + GFF3446 0.50 -2.0 207,740 + GFF3648 0.47 -0.2 208,033 - GFF3447 0.22 +0.5 208,069 - GFF3447 0.28 +0.1 208,190 - GFF3447 0.46 -1.2 208,232 + GFF3447 0.53 -1.3 208,342 - GFF3447 0.70 -0.6 208,479 + +1.0 208,483 + +0.1 208,484 - +1.0 208,537 + -2.8 208,538 - -0.2 208,538 - +1.2 208,538 - +0.3 208,619 + +0.9 208,746 - GFF3448 0.21 +1.0 209,045 + GFF3448 0.55 +0.1 209,073 + GFF3448 0.58 +0.9 209,198 - GFF3448 0.73 -0.6 209,198 - GFF3448 0.73 -0.1 209,230 + GFF3448 0.76 -0.2 209,233 - GFF3448 0.77 +0.4 209,306 - GFF3448 0.85 -0.1 209,338 + GFF3448 0.89 +1.1 209,339 - GFF3448 0.89 -0.3 209,434 + +0.9 209,434 + +0.8 209,435 - +3.2 209,435 - +0.1 209,484 - +0.7 209,513 - -0.1
Or see this region's nucleotide sequence