Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3355 and GFF3356 are separated by 11 nucleotides GFF3356 and GFF3357 are separated by 26 nucleotides GFF3357 and GFF3358 are separated by 20 nucleotides GFF3358 and GFF3359 are separated by 13 nucleotides
GFF3355 - Flagellar basal-body rod protein FlgC, at 120,719 to 121,123
GFF3355
GFF3356 - Flagellar basal-body rod modification protein FlgD, at 121,135 to 121,833
GFF3356
GFF3357 - Flagellar hook protein FlgE, at 121,860 to 123,071
GFF3357
GFF3358 - Flagellar basal-body rod protein FlgF, at 123,092 to 123,847
GFF3358
GFF3359 - Flagellar basal-body rod protein FlgG, at 123,861 to 124,643
GFF3359
Position (kb)
121
122
123
124 Strain fitness (log2 ratio)
-1
0
1
2
3 at 120.950 kb on + strand, within GFF3355 at 120.986 kb on + strand, within GFF3355 at 120.987 kb on - strand, within GFF3355 at 121.052 kb on + strand, within GFF3355 at 121.053 kb on - strand, within GFF3355 at 121.064 kb on - strand, within GFF3355 at 121.135 kb on - strand at 121.140 kb on + strand at 121.152 kb on + strand at 121.395 kb on + strand, within GFF3356 at 121.395 kb on + strand, within GFF3356 at 121.395 kb on + strand, within GFF3356 at 121.395 kb on + strand, within GFF3356 at 121.577 kb on + strand, within GFF3356 at 121.613 kb on + strand, within GFF3356 at 121.614 kb on - strand, within GFF3356 at 121.635 kb on + strand, within GFF3356 at 121.672 kb on + strand, within GFF3356 at 121.792 kb on + strand at 121.839 kb on + strand at 121.840 kb on - strand at 121.840 kb on - strand at 121.845 kb on + strand at 121.925 kb on + strand at 121.947 kb on + strand at 121.956 kb on - strand at 121.956 kb on - strand at 121.982 kb on + strand, within GFF3357 at 122.139 kb on + strand, within GFF3357 at 122.140 kb on - strand, within GFF3357 at 122.229 kb on - strand, within GFF3357 at 122.369 kb on + strand, within GFF3357 at 122.392 kb on - strand, within GFF3357 at 122.433 kb on - strand, within GFF3357 at 122.454 kb on + strand, within GFF3357 at 122.491 kb on - strand, within GFF3357 at 122.544 kb on - strand, within GFF3357 at 122.709 kb on + strand, within GFF3357 at 122.711 kb on + strand, within GFF3357 at 122.981 kb on + strand at 123.003 kb on + strand at 123.004 kb on - strand at 123.309 kb on - strand, within GFF3358 at 123.386 kb on + strand, within GFF3358 at 123.388 kb on + strand, within GFF3358 at 123.694 kb on + strand, within GFF3358 at 123.695 kb on - strand, within GFF3358 at 123.823 kb on + strand at 123.848 kb on + strand at 123.962 kb on + strand, within GFF3359 at 123.997 kb on - strand, within GFF3359
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 120,950 + GFF3355 0.57 +2.5 120,986 + GFF3355 0.66 +2.2 120,987 - GFF3355 0.66 -0.5 121,052 + GFF3355 0.82 +0.6 121,053 - GFF3355 0.82 +0.5 121,064 - GFF3355 0.85 +1.9 121,135 - -0.5 121,140 + +1.9 121,152 + -0.4 121,395 + GFF3356 0.37 -0.6 121,395 + GFF3356 0.37 +1.1 121,395 + GFF3356 0.37 +1.4 121,395 + GFF3356 0.37 -0.2 121,577 + GFF3356 0.63 +1.5 121,613 + GFF3356 0.68 +3.3 121,614 - GFF3356 0.69 +0.4 121,635 + GFF3356 0.72 +1.5 121,672 + GFF3356 0.77 +1.2 121,792 + +0.1 121,839 + -1.1 121,840 - +0.9 121,840 - -0.5 121,845 + +0.8 121,925 + -0.0 121,947 + +1.0 121,956 - +0.4 121,956 - +0.5 121,982 + GFF3357 0.10 +2.0 122,139 + GFF3357 0.23 +0.4 122,140 - GFF3357 0.23 +2.4 122,229 - GFF3357 0.30 +3.0 122,369 + GFF3357 0.42 +0.6 122,392 - GFF3357 0.44 +0.6 122,433 - GFF3357 0.47 +1.0 122,454 + GFF3357 0.49 +0.4 122,491 - GFF3357 0.52 +0.6 122,544 - GFF3357 0.56 +0.0 122,709 + GFF3357 0.70 +1.0 122,711 + GFF3357 0.70 +0.8 122,981 + +0.0 123,003 + +1.2 123,004 - +1.0 123,309 - GFF3358 0.29 +1.8 123,386 + GFF3358 0.39 +0.9 123,388 + GFF3358 0.39 +1.2 123,694 + GFF3358 0.80 +0.7 123,695 - GFF3358 0.80 +0.1 123,823 + +0.7 123,848 + +1.2 123,962 + GFF3359 0.13 +0.8 123,997 - GFF3359 0.17 +0.4
Or see this region's nucleotide sequence