Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3345 and GFF3346 are separated by 236 nucleotides GFF3346 and GFF3347 are separated by 35 nucleotides GFF3347 and GFF3348 are separated by 1 nucleotides
GFF3345 - MFS superfamily export protein YceL, at 113,113 to 114,321
GFF3345
GFF3346 - Ribosomal-protein-S5p-alanine acetyltransferase, at 114,558 to 115,142
GFF3346
GFF3347 - Protein of unknown function YceH, at 115,178 to 115,825
GFF3347
GFF3348 - Virulence factor MviM, at 115,827 to 116,750
GFF3348
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 113.576 kb on + strand, within GFF3345 at 113.576 kb on + strand, within GFF3345 at 113.577 kb on - strand, within GFF3345 at 113.577 kb on - strand, within GFF3345 at 113.577 kb on - strand, within GFF3345 at 113.617 kb on + strand, within GFF3345 at 113.635 kb on + strand, within GFF3345 at 113.658 kb on + strand, within GFF3345 at 113.658 kb on + strand, within GFF3345 at 113.658 kb on + strand, within GFF3345 at 113.660 kb on + strand, within GFF3345 at 113.660 kb on + strand, within GFF3345 at 113.661 kb on - strand, within GFF3345 at 113.753 kb on - strand, within GFF3345 at 113.753 kb on - strand, within GFF3345 at 113.809 kb on - strand, within GFF3345 at 113.845 kb on + strand, within GFF3345 at 114.505 kb on + strand at 114.506 kb on - strand at 114.506 kb on - strand at 114.507 kb on + strand at 114.508 kb on - strand at 114.508 kb on - strand at 114.508 kb on - strand at 114.508 kb on - strand at 114.510 kb on + strand at 114.510 kb on + strand at 114.511 kb on - strand at 114.516 kb on + strand at 114.536 kb on + strand at 114.548 kb on + strand at 114.548 kb on + strand at 114.552 kb on + strand at 114.554 kb on + strand at 114.554 kb on + strand at 114.576 kb on - strand at 114.576 kb on - strand at 114.623 kb on - strand, within GFF3346 at 114.948 kb on + strand, within GFF3346 at 114.949 kb on - strand, within GFF3346 at 114.969 kb on - strand, within GFF3346 at 114.990 kb on + strand, within GFF3346 at 114.990 kb on + strand, within GFF3346 at 115.291 kb on + strand, within GFF3347 at 115.292 kb on - strand, within GFF3347 at 115.490 kb on - strand, within GFF3347 at 115.665 kb on - strand, within GFF3347 at 115.667 kb on - strand, within GFF3347 at 115.783 kb on - strand at 115.806 kb on + strand at 115.894 kb on + strand at 115.946 kb on + strand, within GFF3348 at 115.954 kb on + strand, within GFF3348 at 115.955 kb on - strand, within GFF3348 at 115.979 kb on - strand, within GFF3348 at 115.983 kb on + strand, within GFF3348 at 115.984 kb on - strand, within GFF3348 at 116.042 kb on + strand, within GFF3348
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 113,576 + GFF3345 0.38 +0.2 113,576 + GFF3345 0.38 +0.4 113,577 - GFF3345 0.38 -1.3 113,577 - GFF3345 0.38 -0.6 113,577 - GFF3345 0.38 +0.5 113,617 + GFF3345 0.42 -0.5 113,635 + GFF3345 0.43 -0.5 113,658 + GFF3345 0.45 +0.8 113,658 + GFF3345 0.45 -0.1 113,658 + GFF3345 0.45 -0.1 113,660 + GFF3345 0.45 +0.0 113,660 + GFF3345 0.45 +0.9 113,661 - GFF3345 0.45 +1.4 113,753 - GFF3345 0.53 -0.0 113,753 - GFF3345 0.53 -0.1 113,809 - GFF3345 0.58 +0.5 113,845 + GFF3345 0.61 +1.5 114,505 + -0.4 114,506 - +0.0 114,506 - -0.2 114,507 + +1.2 114,508 - +0.6 114,508 - -1.9 114,508 - +0.5 114,508 - -0.3 114,510 + +0.4 114,510 + -0.0 114,511 - +0.2 114,516 + -0.1 114,536 + +2.4 114,548 + -0.1 114,548 + -0.3 114,552 + -0.7 114,554 + -0.3 114,554 + +0.2 114,576 - +0.2 114,576 - -0.0 114,623 - GFF3346 0.11 +3.2 114,948 + GFF3346 0.67 -0.4 114,949 - GFF3346 0.67 +0.1 114,969 - GFF3346 0.70 +10.0 114,990 + GFF3346 0.74 -0.1 114,990 + GFF3346 0.74 -0.5 115,291 + GFF3347 0.17 -0.2 115,292 - GFF3347 0.18 -0.3 115,490 - GFF3347 0.48 -1.1 115,665 - GFF3347 0.75 -0.1 115,667 - GFF3347 0.75 -0.3 115,783 - +0.7 115,806 + -0.5 115,894 + -0.4 115,946 + GFF3348 0.13 +2.3 115,954 + GFF3348 0.14 +1.8 115,955 - GFF3348 0.14 +0.2 115,979 - GFF3348 0.16 -1.0 115,983 + GFF3348 0.17 +0.5 115,984 - GFF3348 0.17 +2.2 116,042 + GFF3348 0.23 +2.2
Or see this region's nucleotide sequence