Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3182 and GFF3183 are separated by 11 nucleotides GFF3183 and GFF3184 are separated by 262 nucleotides GFF3184 and GFF3185 are separated by 170 nucleotides
GFF3182 - Aerotaxis sensor receptor protein, at 187,890 to 189,410
GFF3182
GFF3183 - FIG01046167: hypothetical protein, at 189,422 to 189,577
GFF3183
GFF3184 - Putrescine aminotransferase (EC 2.6.1.82), at 189,840 to 191,219
GFF3184
GFF3185 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 191,390 to 193,408
GFF3185
Position (kb) 189
190
191
192 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 188.912 kb on + strand, within GFF3182 at 188.913 kb on - strand, within GFF3182 at 189.023 kb on - strand, within GFF3182 at 189.025 kb on - strand, within GFF3182 at 189.025 kb on - strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.077 kb on + strand, within GFF3182 at 189.078 kb on - strand, within GFF3182 at 189.078 kb on - strand, within GFF3182 at 189.078 kb on - strand, within GFF3182 at 189.080 kb on - strand, within GFF3182 at 189.199 kb on + strand, within GFF3182 at 189.199 kb on + strand, within GFF3182 at 189.206 kb on + strand, within GFF3182 at 189.207 kb on - strand, within GFF3182 at 189.323 kb on + strand at 189.323 kb on + strand at 189.325 kb on + strand at 189.325 kb on + strand at 189.325 kb on + strand at 189.326 kb on - strand at 189.326 kb on - strand at 189.345 kb on - strand at 189.359 kb on + strand at 189.360 kb on - strand at 189.360 kb on - strand at 189.360 kb on - strand at 189.381 kb on + strand at 189.538 kb on + strand, within GFF3183 at 189.800 kb on - strand at 189.817 kb on + strand at 189.818 kb on - strand at 189.850 kb on + strand at 189.966 kb on - strand at 189.977 kb on - strand at 190.202 kb on - strand, within GFF3184 at 190.439 kb on + strand, within GFF3184 at 190.481 kb on + strand, within GFF3184 at 190.596 kb on + strand, within GFF3184 at 190.609 kb on + strand, within GFF3184 at 190.613 kb on + strand, within GFF3184 at 190.613 kb on + strand, within GFF3184 at 190.613 kb on + strand, within GFF3184 at 190.658 kb on - strand, within GFF3184 at 190.826 kb on + strand, within GFF3184 at 190.827 kb on - strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.004 kb on - strand, within GFF3184 at 191.091 kb on - strand at 191.099 kb on + strand at 191.245 kb on + strand at 191.336 kb on - strand at 191.361 kb on - strand at 191.431 kb on + strand at 191.432 kb on - strand at 191.661 kb on + strand, within GFF3185 at 191.662 kb on - strand, within GFF3185 at 191.952 kb on - strand, within GFF3185 at 191.983 kb on - strand, within GFF3185 at 191.983 kb on - strand, within GFF3185 at 192.022 kb on + strand, within GFF3185 at 192.123 kb on + strand, within GFF3185 at 192.127 kb on + strand, within GFF3185
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 188,912 + GFF3182 0.67 +0.2 188,913 - GFF3182 0.67 +0.4 189,023 - GFF3182 0.74 +1.6 189,025 - GFF3182 0.75 -0.3 189,025 - GFF3182 0.75 +0.3 189,077 + GFF3182 0.78 +0.8 189,077 + GFF3182 0.78 -0.4 189,077 + GFF3182 0.78 +0.5 189,077 + GFF3182 0.78 +0.1 189,077 + GFF3182 0.78 +0.5 189,078 - GFF3182 0.78 -0.3 189,078 - GFF3182 0.78 -0.3 189,078 - GFF3182 0.78 +0.1 189,080 - GFF3182 0.78 +1.0 189,199 + GFF3182 0.86 -0.1 189,199 + GFF3182 0.86 +0.3 189,206 + GFF3182 0.87 +1.3 189,207 - GFF3182 0.87 -1.5 189,323 + +0.2 189,323 + +0.3 189,325 + +2.2 189,325 + -0.0 189,325 + -0.8 189,326 - -0.2 189,326 - +0.3 189,345 - +0.0 189,359 + +0.7 189,360 - +0.0 189,360 - +0.8 189,360 - +0.1 189,381 + -0.8 189,538 + GFF3183 0.74 +0.5 189,800 - -1.3 189,817 + +0.2 189,818 - -0.8 189,850 + +3.0 189,966 - +0.1 189,977 - +0.0 190,202 - GFF3184 0.26 +0.7 190,439 + GFF3184 0.43 -0.5 190,481 + GFF3184 0.46 -0.1 190,596 + GFF3184 0.55 +1.2 190,609 + GFF3184 0.56 -0.0 190,613 + GFF3184 0.56 -0.6 190,613 + GFF3184 0.56 -0.3 190,613 + GFF3184 0.56 -0.1 190,658 - GFF3184 0.59 +0.2 190,826 + GFF3184 0.71 +8.7 190,827 - GFF3184 0.72 -0.1 191,003 + GFF3184 0.84 +0.5 191,003 + GFF3184 0.84 +0.6 191,003 + GFF3184 0.84 +0.3 191,003 + GFF3184 0.84 -0.9 191,004 - GFF3184 0.84 +0.2 191,091 - -0.7 191,099 + -0.5 191,245 + +0.5 191,336 - -0.4 191,361 - -1.2 191,431 + +0.0 191,432 - +1.2 191,661 + GFF3185 0.13 +1.2 191,662 - GFF3185 0.13 -1.9 191,952 - GFF3185 0.28 -0.0 191,983 - GFF3185 0.29 -0.3 191,983 - GFF3185 0.29 +0.4 192,022 + GFF3185 0.31 +0.6 192,123 + GFF3185 0.36 +6.3 192,127 + GFF3185 0.37 +0.6
Or see this region's nucleotide sequence