Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3161 and GFF3162 are separated by 73 nucleotides GFF3162 and GFF3163 are separated by 96 nucleotides GFF3163 and GFF3164 are separated by 11 nucleotides GFF3164 and GFF3165 are separated by 18 nucleotides
GFF3161 - hypothetical protein, at 164,076 to 164,375
GFF3161
GFF3162 - glycogen synthesis protein GlgS, at 164,449 to 164,658
GFF3162
GFF3163 - hypothetical protein, at 164,755 to 164,904
GFF3163
GFF3164 - Putative inner membrane protein, at 164,916 to 165,536
GFF3164
GFF3165 - Inner membrane protein YqiK, at 165,555 to 167,234
GFF3165
Position (kb) 164
165
166 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 163.953 kb on - strand at 163.955 kb on - strand at 163.985 kb on + strand at 163.985 kb on + strand at 164.037 kb on - strand at 164.048 kb on + strand at 164.049 kb on - strand at 164.405 kb on + strand at 164.405 kb on + strand at 164.406 kb on - strand at 164.450 kb on + strand at 164.541 kb on + strand, within GFF3162 at 164.623 kb on - strand, within GFF3162 at 164.688 kb on + strand at 164.725 kb on - strand at 164.741 kb on + strand at 164.741 kb on + strand at 164.741 kb on + strand at 164.797 kb on + strand, within GFF3163 at 164.797 kb on + strand, within GFF3163 at 164.908 kb on + strand at 164.936 kb on + strand at 164.937 kb on - strand at 165.052 kb on - strand, within GFF3164 at 165.094 kb on - strand, within GFF3164 at 165.405 kb on - strand, within GFF3164 at 165.427 kb on + strand, within GFF3164 at 165.427 kb on + strand, within GFF3164 at 165.427 kb on + strand, within GFF3164 at 165.428 kb on - strand, within GFF3164 at 165.521 kb on + strand at 165.522 kb on - strand at 165.528 kb on + strand at 165.529 kb on - strand at 165.534 kb on + strand at 165.535 kb on - strand at 165.554 kb on - strand at 165.554 kb on - strand at 165.575 kb on - strand at 165.623 kb on - strand at 165.773 kb on + strand, within GFF3165 at 165.773 kb on + strand, within GFF3165 at 165.778 kb on + strand, within GFF3165 at 165.779 kb on - strand, within GFF3165 at 165.863 kb on - strand, within GFF3165 at 165.863 kb on - strand, within GFF3165 at 165.863 kb on - strand, within GFF3165 at 165.868 kb on + strand, within GFF3165 at 166.214 kb on + strand, within GFF3165 at 166.308 kb on - strand, within GFF3165 at 166.499 kb on - strand, within GFF3165 at 166.506 kb on - strand, within GFF3165 at 166.506 kb on - strand, within GFF3165
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 163,953 - -1.2 163,955 - +0.5 163,985 + -0.9 163,985 + -0.0 164,037 - -1.0 164,048 + +0.3 164,049 - +1.0 164,405 + -0.3 164,405 + +0.1 164,406 - -0.0 164,450 + +1.6 164,541 + GFF3162 0.44 -0.0 164,623 - GFF3162 0.83 +1.5 164,688 + +6.5 164,725 - +0.1 164,741 + +0.8 164,741 + -0.2 164,741 + -0.4 164,797 + GFF3163 0.28 -0.1 164,797 + GFF3163 0.28 -0.2 164,908 + +0.0 164,936 + +8.4 164,937 - -0.7 165,052 - GFF3164 0.22 +0.8 165,094 - GFF3164 0.29 +8.4 165,405 - GFF3164 0.79 +1.2 165,427 + GFF3164 0.82 +0.0 165,427 + GFF3164 0.82 -0.1 165,427 + GFF3164 0.82 -1.2 165,428 - GFF3164 0.82 -0.8 165,521 + +0.1 165,522 - -0.3 165,528 + +1.1 165,529 - -0.9 165,534 + +0.1 165,535 - +0.1 165,554 - +1.2 165,554 - -0.6 165,575 - +0.6 165,623 - -0.6 165,773 + GFF3165 0.13 -0.6 165,773 + GFF3165 0.13 -1.3 165,778 + GFF3165 0.13 -0.2 165,779 - GFF3165 0.13 +0.4 165,863 - GFF3165 0.18 -1.9 165,863 - GFF3165 0.18 +0.3 165,863 - GFF3165 0.18 -0.4 165,868 + GFF3165 0.19 -0.1 166,214 + GFF3165 0.39 +0.6 166,308 - GFF3165 0.45 +0.2 166,499 - GFF3165 0.56 -0.0 166,506 - GFF3165 0.57 -0.4 166,506 - GFF3165 0.57 -1.0
Or see this region's nucleotide sequence