Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF300 and GFF301 are separated by 324 nucleotides GFF301 and GFF302 are separated by 30 nucleotides
GFF300 - Deoxyribose operon repressor, DeoR family, at 17,457 to 18,242
GFF300
GFF301 - Ribokinase (EC 2.7.1.15), at 18,567 to 19,487
GFF301
GFF302 - Homolog of fucose/glucose/galactose permeases, at 19,518 to 20,834
GFF302
Position (kb) 18
19
20 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 17.724 kb on - strand, within GFF300 at 17.776 kb on + strand, within GFF300 at 17.832 kb on - strand, within GFF300 at 18.035 kb on + strand, within GFF300 at 18.056 kb on + strand, within GFF300 at 18.071 kb on + strand, within GFF300 at 18.268 kb on + strand at 18.323 kb on + strand at 18.324 kb on - strand at 18.325 kb on + strand at 18.325 kb on + strand at 18.326 kb on - strand at 18.605 kb on + strand at 18.612 kb on + strand at 18.613 kb on - strand at 18.755 kb on + strand, within GFF301 at 18.810 kb on + strand, within GFF301 at 18.810 kb on + strand, within GFF301 at 18.811 kb on - strand, within GFF301 at 18.811 kb on - strand, within GFF301 at 18.811 kb on - strand, within GFF301 at 18.823 kb on + strand, within GFF301 at 19.079 kb on + strand, within GFF301 at 19.086 kb on + strand, within GFF301 at 19.087 kb on - strand, within GFF301 at 19.087 kb on - strand, within GFF301 at 19.157 kb on + strand, within GFF301 at 19.162 kb on - strand, within GFF301 at 19.217 kb on + strand, within GFF301 at 19.217 kb on + strand, within GFF301 at 19.347 kb on + strand, within GFF301 at 19.350 kb on + strand, within GFF301 at 19.351 kb on - strand, within GFF301 at 19.351 kb on - strand, within GFF301 at 19.485 kb on + strand at 19.486 kb on - strand at 19.512 kb on - strand at 19.512 kb on - strand at 19.780 kb on + strand, within GFF302 at 19.781 kb on - strand, within GFF302 at 19.800 kb on + strand, within GFF302 at 19.801 kb on - strand, within GFF302 at 19.801 kb on - strand, within GFF302 at 19.801 kb on - strand, within GFF302 at 19.801 kb on - strand, within GFF302 at 19.815 kb on - strand, within GFF302 at 19.849 kb on - strand, within GFF302 at 19.849 kb on - strand, within GFF302 at 19.921 kb on - strand, within GFF302 at 20.022 kb on + strand, within GFF302 at 20.022 kb on + strand, within GFF302 at 20.132 kb on + strand, within GFF302 at 20.132 kb on + strand, within GFF302 at 20.133 kb on - strand, within GFF302 at 20.136 kb on + strand, within GFF302 at 20.136 kb on + strand, within GFF302 at 20.137 kb on - strand, within GFF302 at 20.144 kb on + strand, within GFF302 at 20.167 kb on + strand, within GFF302 at 20.243 kb on + strand, within GFF302 at 20.254 kb on - strand, within GFF302 at 20.305 kb on - strand, within GFF302 at 20.400 kb on + strand, within GFF302 at 20.401 kb on - strand, within GFF302
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 17,724 - GFF300 0.34 +2.7 17,776 + GFF300 0.41 +0.1 17,832 - GFF300 0.48 +0.7 18,035 + GFF300 0.74 +0.3 18,056 + GFF300 0.76 +0.7 18,071 + GFF300 0.78 +0.9 18,268 + -0.4 18,323 + +0.7 18,324 - +0.6 18,325 + +0.3 18,325 + +0.7 18,326 - +0.2 18,605 + +0.3 18,612 + -0.7 18,613 - -1.0 18,755 + GFF301 0.20 -1.0 18,810 + GFF301 0.26 +0.1 18,810 + GFF301 0.26 -0.0 18,811 - GFF301 0.26 +1.2 18,811 - GFF301 0.26 +0.3 18,811 - GFF301 0.26 -0.3 18,823 + GFF301 0.28 +0.2 19,079 + GFF301 0.56 +0.1 19,086 + GFF301 0.56 -0.1 19,087 - GFF301 0.56 -0.2 19,087 - GFF301 0.56 +0.2 19,157 + GFF301 0.64 +0.9 19,162 - GFF301 0.65 +0.3 19,217 + GFF301 0.71 +1.9 19,217 + GFF301 0.71 +0.5 19,347 + GFF301 0.85 +0.2 19,350 + GFF301 0.85 -0.4 19,351 - GFF301 0.85 -0.9 19,351 - GFF301 0.85 +0.5 19,485 + -0.3 19,486 - -0.4 19,512 - +0.3 19,512 - +0.9 19,780 + GFF302 0.20 -0.3 19,781 - GFF302 0.20 -1.2 19,800 + GFF302 0.21 +1.2 19,801 - GFF302 0.21 +0.1 19,801 - GFF302 0.21 +5.8 19,801 - GFF302 0.21 -1.7 19,801 - GFF302 0.21 +5.9 19,815 - GFF302 0.23 +0.0 19,849 - GFF302 0.25 +0.0 19,849 - GFF302 0.25 +0.2 19,921 - GFF302 0.31 +1.5 20,022 + GFF302 0.38 -0.7 20,022 + GFF302 0.38 +0.5 20,132 + GFF302 0.47 +0.2 20,132 + GFF302 0.47 -0.2 20,133 - GFF302 0.47 +1.2 20,136 + GFF302 0.47 -0.2 20,136 + GFF302 0.47 -1.0 20,137 - GFF302 0.47 +1.1 20,144 + GFF302 0.48 +0.4 20,167 + GFF302 0.49 +0.7 20,243 + GFF302 0.55 +0.0 20,254 - GFF302 0.56 -0.3 20,305 - GFF302 0.60 +0.1 20,400 + GFF302 0.67 +1.1 20,401 - GFF302 0.67 -1.4
Or see this region's nucleotide sequence