Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2913 and GFF2914 are separated by 85 nucleotides GFF2914 and GFF2978 are separated by 335 nucleotides GFF2978 and GFF2915 are separated by 13 nucleotides GFF2915 and GFF2916 are separated by 222 nucleotides
GFF2913 - Argininosuccinate synthase (EC 6.3.4.5), at 399 to 1,742
GFF2913
GFF2914 - putative cytoplasmic protein, at 1,828 to 2,892
GFF2914
GFF2978 - tRNA-Leu-GAG, at 3,228 to 3,314
GFF2978
GFF2915 - Preprotein translocase subunit SecG (TC 3.A.5.1.1), at 3,328 to 3,660
GFF2915
GFF2916 - Phosphoglucosamine mutase (EC 5.4.2.10), at 3,883 to 5,220
GFF2916
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 0.859 kb on - strand, within GFF2913 at 0.885 kb on - strand, within GFF2913 at 0.948 kb on - strand, within GFF2913 at 1.542 kb on + strand, within GFF2913 at 1.542 kb on + strand, within GFF2913 at 1.543 kb on - strand, within GFF2913 at 1.543 kb on - strand, within GFF2913 at 1.544 kb on + strand, within GFF2913 at 1.582 kb on + strand, within GFF2913 at 1.791 kb on + strand at 1.897 kb on + strand at 1.898 kb on - strand at 1.898 kb on - strand at 1.898 kb on - strand at 1.899 kb on + strand at 1.998 kb on + strand, within GFF2914 at 2.029 kb on - strand, within GFF2914 at 2.139 kb on - strand, within GFF2914 at 2.224 kb on - strand, within GFF2914 at 2.256 kb on + strand, within GFF2914 at 2.304 kb on + strand, within GFF2914 at 2.305 kb on - strand, within GFF2914 at 2.309 kb on - strand, within GFF2914 at 2.355 kb on + strand, within GFF2914 at 2.356 kb on - strand, within GFF2914 at 2.528 kb on + strand, within GFF2914 at 2.654 kb on - strand, within GFF2914 at 2.656 kb on + strand, within GFF2914 at 2.906 kb on - strand at 2.960 kb on - strand at 2.960 kb on - strand at 2.960 kb on - strand at 2.978 kb on + strand at 2.994 kb on + strand at 2.994 kb on + strand at 2.996 kb on + strand at 2.996 kb on + strand at 3.008 kb on + strand at 3.009 kb on - strand at 3.084 kb on + strand at 3.084 kb on + strand at 3.084 kb on + strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.085 kb on - strand at 3.093 kb on - strand at 3.093 kb on - strand at 3.093 kb on - strand at 3.096 kb on + strand at 3.096 kb on + strand at 3.096 kb on + strand at 3.096 kb on + strand at 3.096 kb on + strand at 3.096 kb on + strand at 3.097 kb on - strand at 3.097 kb on - strand at 3.100 kb on + strand at 3.101 kb on - strand at 3.106 kb on + strand at 3.116 kb on + strand at 3.439 kb on - strand, within GFF2915 at 3.787 kb on + strand at 3.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 859 - GFF2913 0.34 +1.1 885 - GFF2913 0.36 -1.0 948 - GFF2913 0.41 +1.7 1,542 + GFF2913 0.85 -0.3 1,542 + GFF2913 0.85 +0.3 1,543 - GFF2913 0.85 +1.3 1,543 - GFF2913 0.85 +2.8 1,544 + GFF2913 0.85 +0.5 1,582 + GFF2913 0.88 -2.1 1,791 + -2.0 1,897 + +0.9 1,898 - -0.3 1,898 - +1.3 1,898 - +0.1 1,899 + +0.2 1,998 + GFF2914 0.16 -0.1 2,029 - GFF2914 0.19 +1.2 2,139 - GFF2914 0.29 +1.0 2,224 - GFF2914 0.37 +1.1 2,256 + GFF2914 0.40 +1.5 2,304 + GFF2914 0.45 -0.0 2,305 - GFF2914 0.45 +0.2 2,309 - GFF2914 0.45 +0.4 2,355 + GFF2914 0.49 -0.0 2,356 - GFF2914 0.50 +1.1 2,528 + GFF2914 0.66 -0.4 2,654 - GFF2914 0.78 +1.1 2,656 + GFF2914 0.78 +0.3 2,906 - -0.6 2,960 - +0.2 2,960 - +0.2 2,960 - +0.6 2,978 + +0.2 2,994 + +1.2 2,994 + -0.4 2,996 + -0.7 2,996 + -0.4 3,008 + -0.1 3,009 - -0.2 3,084 + +0.3 3,084 + +2.1 3,084 + -0.2 3,085 - +0.0 3,085 - -1.6 3,085 - -0.7 3,085 - +0.3 3,085 - -0.3 3,093 - -0.1 3,093 - -0.8 3,093 - +1.5 3,096 + +1.1 3,096 + -0.0 3,096 + -0.0 3,096 + +1.5 3,096 + +0.2 3,096 + +0.3 3,097 - +1.4 3,097 - +0.2 3,100 + -0.6 3,101 - -0.1 3,106 + +1.5 3,116 + -0.5 3,439 - GFF2915 0.33 -0.4 3,787 + -0.1 3,877 - -0.0
Or see this region's nucleotide sequence