Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2902 and GFF2903 are separated by 17 nucleotides GFF2903 and GFF2904 overlap by 4 nucleotides GFF2904 and GFF2905 are separated by 589 nucleotides
GFF2902 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 29,364 to 30,293
GFF2902
GFF2903 - Acetolactate synthase small subunit (EC 2.2.1.6), at 30,311 to 30,574
GFF2903
GFF2904 - Acetolactate synthase large subunit (EC 2.2.1.6), at 30,571 to 32,217
GFF2904
GFF2905 - MG(2+) CHELATASE FAMILY PROTEIN / ComM-related protein, at 32,807 to 34,327
GFF2905
Position (kb)
30
31
32
33 Strain fitness (log2 ratio)
-1
0
1
2 at 29.591 kb on + strand, within GFF2902 at 29.730 kb on + strand, within GFF2902 at 29.800 kb on + strand, within GFF2902 at 29.905 kb on + strand, within GFF2902 at 29.906 kb on - strand, within GFF2902 at 29.948 kb on - strand, within GFF2902 at 29.991 kb on + strand, within GFF2902 at 30.007 kb on + strand, within GFF2902 at 30.007 kb on + strand, within GFF2902 at 30.007 kb on + strand, within GFF2902 at 30.122 kb on + strand, within GFF2902 at 30.122 kb on + strand, within GFF2902 at 30.122 kb on + strand, within GFF2902 at 30.122 kb on + strand, within GFF2902 at 30.123 kb on - strand, within GFF2902 at 30.123 kb on - strand, within GFF2902 at 30.195 kb on - strand, within GFF2902 at 30.271 kb on + strand at 30.380 kb on - strand, within GFF2903 at 30.383 kb on + strand, within GFF2903 at 30.384 kb on - strand, within GFF2903 at 30.384 kb on - strand, within GFF2903 at 30.391 kb on + strand, within GFF2903 at 30.392 kb on - strand, within GFF2903 at 30.399 kb on - strand, within GFF2903 at 30.440 kb on + strand, within GFF2903 at 30.524 kb on + strand, within GFF2903 at 30.525 kb on - strand, within GFF2903 at 30.552 kb on - strand at 30.718 kb on - strand at 30.724 kb on - strand at 30.862 kb on + strand, within GFF2904 at 30.896 kb on - strand, within GFF2904 at 30.945 kb on + strand, within GFF2904 at 31.055 kb on + strand, within GFF2904 at 31.056 kb on - strand, within GFF2904 at 31.056 kb on - strand, within GFF2904 at 31.062 kb on + strand, within GFF2904 at 31.062 kb on + strand, within GFF2904 at 31.062 kb on + strand, within GFF2904 at 31.062 kb on + strand, within GFF2904 at 31.063 kb on - strand, within GFF2904 at 31.063 kb on - strand, within GFF2904 at 31.160 kb on + strand, within GFF2904 at 31.424 kb on - strand, within GFF2904 at 31.424 kb on - strand, within GFF2904 at 31.458 kb on + strand, within GFF2904 at 31.488 kb on - strand, within GFF2904 at 31.593 kb on + strand, within GFF2904 at 31.614 kb on - strand, within GFF2904 at 31.738 kb on - strand, within GFF2904 at 32.038 kb on - strand, within GFF2904 at 32.048 kb on + strand, within GFF2904 at 32.114 kb on + strand at 32.114 kb on + strand at 32.115 kb on - strand at 32.137 kb on - strand at 32.161 kb on + strand at 32.194 kb on + strand at 32.218 kb on - strand at 32.646 kb on - strand at 32.646 kb on - strand at 32.646 kb on - strand at 32.806 kb on + strand at 32.807 kb on - strand at 32.866 kb on + strand at 32.866 kb on + strand at 32.867 kb on - strand at 32.867 kb on - strand at 32.867 kb on - strand at 32.868 kb on + strand at 32.868 kb on + strand at 32.869 kb on - strand at 32.869 kb on - strand at 32.878 kb on + strand at 32.932 kb on - strand at 32.953 kb on - strand at 32.953 kb on - strand at 32.957 kb on - strand at 32.957 kb on - strand at 32.969 kb on - strand, within GFF2905 at 33.094 kb on + strand, within GFF2905 at 33.095 kb on - strand, within GFF2905 at 33.116 kb on + strand, within GFF2905 at 33.142 kb on + strand, within GFF2905
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 29,591 + GFF2902 0.24 +0.1 29,730 + GFF2902 0.39 -0.4 29,800 + GFF2902 0.47 +0.3 29,905 + GFF2902 0.58 -1.0 29,906 - GFF2902 0.58 +2.4 29,948 - GFF2902 0.63 +0.4 29,991 + GFF2902 0.67 +1.2 30,007 + GFF2902 0.69 -0.3 30,007 + GFF2902 0.69 +1.6 30,007 + GFF2902 0.69 +0.4 30,122 + GFF2902 0.82 +0.4 30,122 + GFF2902 0.82 -0.3 30,122 + GFF2902 0.82 -1.5 30,122 + GFF2902 0.82 -0.2 30,123 - GFF2902 0.82 +0.9 30,123 - GFF2902 0.82 +1.4 30,195 - GFF2902 0.89 +1.5 30,271 + +0.3 30,380 - GFF2903 0.26 -0.5 30,383 + GFF2903 0.27 +0.1 30,384 - GFF2903 0.28 -0.3 30,384 - GFF2903 0.28 -0.8 30,391 + GFF2903 0.30 +0.5 30,392 - GFF2903 0.31 -0.1 30,399 - GFF2903 0.33 +0.3 30,440 + GFF2903 0.49 -0.3 30,524 + GFF2903 0.81 +0.7 30,525 - GFF2903 0.81 -1.0 30,552 - +2.3 30,718 - +1.2 30,724 - +1.4 30,862 + GFF2904 0.18 -0.5 30,896 - GFF2904 0.20 +0.6 30,945 + GFF2904 0.23 -0.3 31,055 + GFF2904 0.29 +0.8 31,056 - GFF2904 0.29 +0.5 31,056 - GFF2904 0.29 +0.4 31,062 + GFF2904 0.30 +0.0 31,062 + GFF2904 0.30 -0.6 31,062 + GFF2904 0.30 +0.5 31,062 + GFF2904 0.30 +1.0 31,063 - GFF2904 0.30 +1.2 31,063 - GFF2904 0.30 +0.3 31,160 + GFF2904 0.36 +0.2 31,424 - GFF2904 0.52 +0.2 31,424 - GFF2904 0.52 -1.0 31,458 + GFF2904 0.54 -0.4 31,488 - GFF2904 0.56 +0.8 31,593 + GFF2904 0.62 -1.4 31,614 - GFF2904 0.63 +0.1 31,738 - GFF2904 0.71 +0.8 32,038 - GFF2904 0.89 -1.4 32,048 + GFF2904 0.90 -1.2 32,114 + +0.6 32,114 + +0.1 32,115 - -0.9 32,137 - +0.4 32,161 + +0.4 32,194 + -0.4 32,218 - +2.0 32,646 - -0.8 32,646 - -0.5 32,646 - -0.8 32,806 + +1.0 32,807 - +2.1 32,866 + -0.0 32,866 + +0.4 32,867 - +0.5 32,867 - -1.7 32,867 - +1.3 32,868 + -0.3 32,868 + +1.3 32,869 - -0.1 32,869 - +2.4 32,878 + +0.5 32,932 - +0.3 32,953 - -0.0 32,953 - -0.7 32,957 - +0.4 32,957 - +1.0 32,969 - GFF2905 0.11 +0.2 33,094 + GFF2905 0.19 +1.3 33,095 - GFF2905 0.19 -0.1 33,116 + GFF2905 0.20 -0.3 33,142 + GFF2905 0.22 +1.4
Or see this region's nucleotide sequence