Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2897 and GFF2898 are separated by 2 nucleotides GFF2898 and GFF2899 overlap by 4 nucleotides GFF2899 and GFF2900 are separated by 283 nucleotides GFF2900 and GFF2901 are separated by 2 nucleotides
GFF2897 - HTH-type transcriptional regulator IlvY, at 23,977 to 24,864
GFF2897
GFF2898 - FIG074102: hypothetical protein, at 24,867 to 25,208
GFF2898
GFF2899 - Phage-related protein, at 25,205 to 25,522
GFF2899
GFF2900 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 25,806 to 27,350
GFF2900
GFF2901 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 27,353 to 29,203
GFF2901
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9 at 24.876 kb on + strand at 24.915 kb on - strand, within GFF2898 at 24.987 kb on - strand, within GFF2898 at 24.987 kb on - strand, within GFF2898 at 24.999 kb on - strand, within GFF2898 at 25.056 kb on + strand, within GFF2898 at 25.057 kb on - strand, within GFF2898 at 25.057 kb on - strand, within GFF2898 at 25.130 kb on + strand, within GFF2898 at 25.240 kb on + strand, within GFF2899 at 25.304 kb on - strand, within GFF2899 at 25.341 kb on + strand, within GFF2899 at 25.341 kb on + strand, within GFF2899 at 25.342 kb on - strand, within GFF2899 at 25.342 kb on - strand, within GFF2899 at 25.346 kb on - strand, within GFF2899 at 25.358 kb on - strand, within GFF2899 at 25.484 kb on + strand, within GFF2899 at 25.568 kb on - strand at 25.635 kb on + strand at 25.636 kb on - strand at 25.749 kb on + strand at 25.995 kb on - strand, within GFF2900 at 25.995 kb on - strand, within GFF2900 at 26.042 kb on + strand, within GFF2900 at 26.044 kb on + strand, within GFF2900 at 26.044 kb on + strand, within GFF2900 at 26.045 kb on - strand, within GFF2900 at 26.059 kb on - strand, within GFF2900 at 26.089 kb on + strand, within GFF2900 at 26.090 kb on - strand, within GFF2900 at 26.138 kb on + strand, within GFF2900 at 26.139 kb on - strand, within GFF2900 at 26.375 kb on - strand, within GFF2900 at 26.456 kb on - strand, within GFF2900 at 26.543 kb on + strand, within GFF2900 at 26.562 kb on - strand, within GFF2900 at 26.562 kb on - strand, within GFF2900 at 26.562 kb on - strand, within GFF2900 at 26.562 kb on - strand, within GFF2900 at 26.562 kb on - strand, within GFF2900 at 26.564 kb on + strand, within GFF2900 at 26.626 kb on - strand, within GFF2900 at 27.167 kb on + strand, within GFF2900 at 27.168 kb on - strand, within GFF2900 at 27.173 kb on + strand, within GFF2900 at 27.174 kb on - strand, within GFF2900 at 27.174 kb on - strand, within GFF2900 at 27.174 kb on - strand, within GFF2900 at 27.174 kb on - strand, within GFF2900 at 27.178 kb on - strand, within GFF2900 at 27.275 kb on - strand at 27.275 kb on - strand at 27.289 kb on - strand at 27.592 kb on + strand, within GFF2901 at 27.723 kb on + strand, within GFF2901 at 27.841 kb on - strand, within GFF2901 at 27.957 kb on + strand, within GFF2901 at 27.958 kb on - strand, within GFF2901 at 27.958 kb on - strand, within GFF2901 at 27.964 kb on - strand, within GFF2901 at 27.964 kb on - strand, within GFF2901 at 28.043 kb on - strand, within GFF2901 at 28.114 kb on - strand, within GFF2901 at 28.114 kb on - strand, within GFF2901 at 28.238 kb on - strand, within GFF2901 at 28.242 kb on + strand, within GFF2901 at 28.243 kb on - strand, within GFF2901 at 28.268 kb on - strand, within GFF2901
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 24,876 + -0.5 24,915 - GFF2898 0.14 -1.2 24,987 - GFF2898 0.35 +0.6 24,987 - GFF2898 0.35 +0.8 24,999 - GFF2898 0.39 -0.3 25,056 + GFF2898 0.55 -0.3 25,057 - GFF2898 0.56 -0.1 25,057 - GFF2898 0.56 +0.3 25,130 + GFF2898 0.77 +0.8 25,240 + GFF2899 0.11 -1.7 25,304 - GFF2899 0.31 -0.7 25,341 + GFF2899 0.43 +1.0 25,341 + GFF2899 0.43 -0.0 25,342 - GFF2899 0.43 +0.7 25,342 - GFF2899 0.43 +0.6 25,346 - GFF2899 0.44 +0.3 25,358 - GFF2899 0.48 +0.2 25,484 + GFF2899 0.88 -2.3 25,568 - +0.6 25,635 + +0.1 25,636 - +0.5 25,749 + -0.2 25,995 - GFF2900 0.12 +0.2 25,995 - GFF2900 0.12 -0.1 26,042 + GFF2900 0.15 -0.2 26,044 + GFF2900 0.15 +0.2 26,044 + GFF2900 0.15 -0.5 26,045 - GFF2900 0.15 +0.8 26,059 - GFF2900 0.16 +1.6 26,089 + GFF2900 0.18 +0.4 26,090 - GFF2900 0.18 +1.5 26,138 + GFF2900 0.21 -1.0 26,139 - GFF2900 0.22 +0.3 26,375 - GFF2900 0.37 +0.5 26,456 - GFF2900 0.42 -0.4 26,543 + GFF2900 0.48 +1.1 26,562 - GFF2900 0.49 +1.5 26,562 - GFF2900 0.49 +2.0 26,562 - GFF2900 0.49 -0.4 26,562 - GFF2900 0.49 -0.3 26,562 - GFF2900 0.49 -0.0 26,564 + GFF2900 0.49 -0.5 26,626 - GFF2900 0.53 -0.6 27,167 + GFF2900 0.88 -1.8 27,168 - GFF2900 0.88 -1.6 27,173 + GFF2900 0.88 +0.6 27,174 - GFF2900 0.89 +0.1 27,174 - GFF2900 0.89 +0.8 27,174 - GFF2900 0.89 -1.7 27,174 - GFF2900 0.89 +0.6 27,178 - GFF2900 0.89 +9.4 27,275 - +0.7 27,275 - -0.7 27,289 - -0.8 27,592 + GFF2901 0.13 +0.3 27,723 + GFF2901 0.20 +0.6 27,841 - GFF2901 0.26 -0.5 27,957 + GFF2901 0.33 -0.9 27,958 - GFF2901 0.33 -0.0 27,958 - GFF2901 0.33 -0.0 27,964 - GFF2901 0.33 +2.1 27,964 - GFF2901 0.33 -1.1 28,043 - GFF2901 0.37 +0.3 28,114 - GFF2901 0.41 -0.0 28,114 - GFF2901 0.41 -0.2 28,238 - GFF2901 0.48 +1.0 28,242 + GFF2901 0.48 +1.0 28,243 - GFF2901 0.48 -0.1 28,268 - GFF2901 0.49 +1.0
Or see this region's nucleotide sequence