Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF280 and GFF281 are separated by 121 nucleotides GFF281 and GFF282 are separated by 1 nucleotides GFF282 and GFF283 overlap by 2 nucleotides GFF283 and GFF284 are separated by 257 nucleotides
GFF280 - Cytochrome c heme lyase subunit CcmL / Cytochrome c heme lyase subunit CcmH, at 29 to 757
GFF280
GFF281 - Uncharacterized protein YidR, at 879 to 2,105
GFF281
GFF282 - Outer membrane lipoprotein YidQ, at 2,107 to 2,367
GFF282
GFF283 - hypothetical protein, at 2,366 to 2,485
GFF283
GFF284 - 16 kDa heat shock protein A, at 2,743 to 3,162
GFF284
Position (kb) 0
1
2
3 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 0.218 kb on + strand, within GFF280 at 0.219 kb on - strand, within GFF280 at 0.426 kb on + strand, within GFF280 at 0.459 kb on - strand, within GFF280 at 0.613 kb on - strand, within GFF280 at 0.620 kb on + strand, within GFF280 at 0.621 kb on - strand, within GFF280 at 0.621 kb on - strand, within GFF280 at 0.815 kb on - strand at 0.815 kb on - strand at 0.843 kb on + strand at 0.843 kb on + strand at 0.844 kb on - strand at 0.917 kb on + strand at 0.917 kb on + strand at 0.918 kb on - strand at 0.918 kb on - strand at 0.929 kb on + strand at 0.968 kb on - strand at 0.968 kb on - strand at 1.104 kb on + strand, within GFF281 at 1.104 kb on + strand, within GFF281 at 1.104 kb on + strand, within GFF281 at 1.112 kb on + strand, within GFF281 at 1.200 kb on - strand, within GFF281 at 1.220 kb on + strand, within GFF281 at 1.221 kb on - strand, within GFF281 at 1.301 kb on + strand, within GFF281 at 1.301 kb on + strand, within GFF281 at 1.305 kb on + strand, within GFF281 at 1.307 kb on + strand, within GFF281 at 1.421 kb on + strand, within GFF281 at 1.422 kb on - strand, within GFF281 at 1.485 kb on - strand, within GFF281 at 1.591 kb on + strand, within GFF281 at 1.694 kb on + strand, within GFF281 at 1.700 kb on + strand, within GFF281 at 1.758 kb on - strand, within GFF281 at 1.856 kb on - strand, within GFF281 at 1.876 kb on + strand, within GFF281 at 1.890 kb on - strand, within GFF281 at 2.080 kb on + strand at 2.081 kb on - strand at 2.104 kb on - strand at 2.232 kb on + strand, within GFF282 at 2.232 kb on + strand, within GFF282 at 2.336 kb on + strand, within GFF282 at 2.336 kb on + strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.390 kb on - strand, within GFF283 at 2.390 kb on - strand, within GFF283 at 2.410 kb on + strand, within GFF283 at 2.481 kb on + strand at 2.484 kb on - strand at 2.613 kb on + strand at 2.624 kb on + strand at 2.628 kb on + strand at 2.642 kb on + strand at 2.644 kb on + strand at 2.651 kb on + strand at 2.652 kb on - strand at 2.742 kb on + strand at 2.823 kb on + strand, within GFF284 at 2.887 kb on + strand, within GFF284 at 2.997 kb on + strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.002 kb on - strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.101 kb on - strand, within GFF284 at 3.102 kb on + strand, within GFF284
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 218 + GFF280 0.26 -0.8 219 - GFF280 0.26 +1.1 426 + GFF280 0.54 +0.3 459 - GFF280 0.59 +0.1 613 - GFF280 0.80 +0.2 620 + GFF280 0.81 +0.8 621 - GFF280 0.81 +0.3 621 - GFF280 0.81 +0.7 815 - -0.2 815 - -0.5 843 + -1.0 843 + +0.4 844 - +0.3 917 + -1.1 917 + -0.2 918 - -0.1 918 - +0.1 929 + +0.0 968 - +2.6 968 - +0.1 1,104 + GFF281 0.18 +6.9 1,104 + GFF281 0.18 +3.0 1,104 + GFF281 0.18 -0.2 1,112 + GFF281 0.19 -1.1 1,200 - GFF281 0.26 +0.5 1,220 + GFF281 0.28 -0.2 1,221 - GFF281 0.28 +0.6 1,301 + GFF281 0.34 -0.8 1,301 + GFF281 0.34 +0.2 1,305 + GFF281 0.35 +1.6 1,307 + GFF281 0.35 +6.8 1,421 + GFF281 0.44 +0.1 1,422 - GFF281 0.44 -1.0 1,485 - GFF281 0.49 +0.3 1,591 + GFF281 0.58 +0.7 1,694 + GFF281 0.66 -1.6 1,700 + GFF281 0.67 +0.5 1,758 - GFF281 0.72 -1.0 1,856 - GFF281 0.80 -0.4 1,876 + GFF281 0.81 -1.4 1,890 - GFF281 0.82 +3.9 2,080 + -0.2 2,081 - +0.6 2,104 - +2.0 2,232 + GFF282 0.48 +0.2 2,232 + GFF282 0.48 -0.0 2,336 + GFF282 0.88 +4.3 2,336 + GFF282 0.88 -1.8 2,341 - GFF282 0.90 +0.6 2,341 - GFF282 0.90 -0.3 2,341 - GFF282 0.90 +0.2 2,390 - GFF283 0.20 -1.3 2,390 - GFF283 0.20 -0.5 2,410 + GFF283 0.37 +1.1 2,481 + -0.4 2,484 - +0.4 2,613 + -0.3 2,624 + +1.7 2,628 + -0.7 2,642 + +0.6 2,644 + -0.9 2,651 + +1.4 2,652 - +1.5 2,742 + +0.5 2,823 + GFF284 0.19 +0.9 2,887 + GFF284 0.34 -0.3 2,997 + GFF284 0.60 +1.1 2,998 - GFF284 0.61 -0.3 2,998 - GFF284 0.61 +6.5 3,001 + GFF284 0.61 -1.4 3,001 + GFF284 0.61 +0.5 3,002 - GFF284 0.62 -0.2 3,039 + GFF284 0.70 -0.5 3,039 + GFF284 0.70 +1.0 3,100 + GFF284 0.85 +1.9 3,100 + GFF284 0.85 -0.4 3,101 - GFF284 0.85 +2.2 3,102 + GFF284 0.85 +0.1
Or see this region's nucleotide sequence