Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2770 and GFF2771 are separated by 37 nucleotides GFF2771 and GFF2772 are separated by 3 nucleotides GFF2772 and GFF2773 are separated by 8 nucleotides GFF2773 and GFF2774 overlap by 1 nucleotides
GFF2770 - 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55), at 295,676 to 296,530
GFF2770
GFF2771 - FIG002708: Protein SirB1, at 296,568 to 297,377
GFF2771
GFF2772 - FIG002082: Protein SirB2, at 297,381 to 297,758
GFF2772
GFF2773 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 297,767 to 298,600
GFF2773
GFF2774 - Peptide chain release factor 1, at 298,600 to 299,682
GFF2774
Position (kb) 297
298 Strain fitness (log2 ratio) -1
0
1
2
3 at 296.665 kb on - strand, within GFF2771 at 296.731 kb on + strand, within GFF2771 at 296.898 kb on - strand, within GFF2771 at 296.900 kb on + strand, within GFF2771 at 296.901 kb on - strand, within GFF2771 at 297.063 kb on + strand, within GFF2771 at 297.428 kb on - strand, within GFF2772 at 297.428 kb on - strand, within GFF2772 at 297.503 kb on + strand, within GFF2772 at 297.504 kb on - strand, within GFF2772 at 297.626 kb on + strand, within GFF2772 at 297.630 kb on + strand, within GFF2772 at 297.630 kb on + strand, within GFF2772 at 297.630 kb on + strand, within GFF2772 at 297.828 kb on - strand at 297.943 kb on + strand, within GFF2773 at 297.943 kb on + strand, within GFF2773 at 297.949 kb on + strand, within GFF2773 at 298.004 kb on + strand, within GFF2773 at 298.114 kb on + strand, within GFF2773 at 298.114 kb on + strand, within GFF2773 at 298.114 kb on + strand, within GFF2773 at 298.115 kb on - strand, within GFF2773 at 298.489 kb on - strand, within GFF2773 at 298.489 kb on - strand, within GFF2773 at 298.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 296,665 - GFF2771 0.12 -1.1 296,731 + GFF2771 0.20 +0.9 296,898 - GFF2771 0.41 -1.1 296,900 + GFF2771 0.41 -0.4 296,901 - GFF2771 0.41 +1.3 297,063 + GFF2771 0.61 +0.4 297,428 - GFF2772 0.12 +0.8 297,428 - GFF2772 0.12 +0.5 297,503 + GFF2772 0.32 +0.7 297,504 - GFF2772 0.33 -0.5 297,626 + GFF2772 0.65 +0.7 297,630 + GFF2772 0.66 +1.6 297,630 + GFF2772 0.66 +3.1 297,630 + GFF2772 0.66 -0.0 297,828 - +0.8 297,943 + GFF2773 0.21 +0.3 297,943 + GFF2773 0.21 +1.0 297,949 + GFF2773 0.22 +1.3 298,004 + GFF2773 0.28 +2.4 298,114 + GFF2773 0.42 -0.8 298,114 + GFF2773 0.42 +1.4 298,114 + GFF2773 0.42 -0.0 298,115 - GFF2773 0.42 +0.7 298,489 - GFF2773 0.87 +0.7 298,489 - GFF2773 0.87 -0.2 298,602 - -0.7
Or see this region's nucleotide sequence