Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2462 and GFF2463 are separated by 90 nucleotides GFF2463 and GFF2464 are separated by 3 nucleotides GFF2464 and GFF2465 are separated by 10 nucleotides
GFF2462 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.-), at 82,477 to 82,962
GFF2462
GFF2463 - Hypothetical protein, similar to phosphoserine phosphatase, at 83,053 to 83,688
GFF2463
GFF2464 - PTS system, IIB component (EC 2.7.1.69) / PTS system, IIC component (EC 2.7.1.69), at 83,692 to 85,053
GFF2464
GFF2465 - N-acetylmuramic acid 6-phosphate etherase, at 85,064 to 85,957
GFF2465
Position (kb) 83
84
85
86 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9 at 83.144 kb on + strand, within GFF2463 at 83.293 kb on - strand, within GFF2463 at 83.365 kb on + strand, within GFF2463 at 83.389 kb on + strand, within GFF2463 at 83.567 kb on - strand, within GFF2463 at 83.625 kb on + strand at 83.625 kb on + strand at 83.639 kb on - strand at 83.765 kb on - strand at 83.983 kb on - strand, within GFF2464 at 83.983 kb on - strand, within GFF2464 at 83.983 kb on - strand, within GFF2464 at 84.087 kb on - strand, within GFF2464 at 84.118 kb on - strand, within GFF2464 at 84.571 kb on + strand, within GFF2464 at 84.694 kb on + strand, within GFF2464 at 84.694 kb on + strand, within GFF2464 at 84.694 kb on + strand, within GFF2464 at 84.742 kb on - strand, within GFF2464 at 84.743 kb on + strand, within GFF2464 at 84.793 kb on + strand, within GFF2464 at 84.886 kb on + strand, within GFF2464 at 84.887 kb on - strand, within GFF2464 at 84.890 kb on + strand, within GFF2464 at 84.891 kb on - strand, within GFF2464 at 84.916 kb on + strand, within GFF2464 at 84.950 kb on - strand at 85.019 kb on - strand at 85.048 kb on - strand at 85.053 kb on + strand at 85.053 kb on + strand at 85.054 kb on - strand at 85.054 kb on - strand at 85.299 kb on + strand, within GFF2465 at 85.339 kb on + strand, within GFF2465 at 85.427 kb on - strand, within GFF2465 at 85.429 kb on + strand, within GFF2465 at 85.521 kb on + strand, within GFF2465 at 85.757 kb on - strand, within GFF2465 at 85.766 kb on - strand, within GFF2465 at 85.855 kb on + strand, within GFF2465 at 85.868 kb on - strand at 85.876 kb on + strand at 85.891 kb on + strand at 85.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 83,144 + GFF2463 0.14 +1.7 83,293 - GFF2463 0.38 -0.4 83,365 + GFF2463 0.49 +1.0 83,389 + GFF2463 0.53 -0.4 83,567 - GFF2463 0.81 -0.4 83,625 + +0.4 83,625 + +1.7 83,639 - +0.4 83,765 - +1.0 83,983 - GFF2464 0.21 +1.2 83,983 - GFF2464 0.21 -0.1 83,983 - GFF2464 0.21 +0.2 84,087 - GFF2464 0.29 +0.1 84,118 - GFF2464 0.31 -0.5 84,571 + GFF2464 0.65 -0.5 84,694 + GFF2464 0.74 -0.9 84,694 + GFF2464 0.74 -1.0 84,694 + GFF2464 0.74 +0.3 84,742 - GFF2464 0.77 -0.2 84,743 + GFF2464 0.77 +0.1 84,793 + GFF2464 0.81 -0.0 84,886 + GFF2464 0.88 +0.4 84,887 - GFF2464 0.88 -0.7 84,890 + GFF2464 0.88 +1.0 84,891 - GFF2464 0.88 -0.4 84,916 + GFF2464 0.90 -0.3 84,950 - -0.6 85,019 - -0.5 85,048 - +1.7 85,053 + -0.4 85,053 + -0.4 85,054 - +1.0 85,054 - -1.2 85,299 + GFF2465 0.26 +0.5 85,339 + GFF2465 0.31 -0.4 85,427 - GFF2465 0.41 -1.2 85,429 + GFF2465 0.41 +0.7 85,521 + GFF2465 0.51 +0.8 85,757 - GFF2465 0.78 -0.0 85,766 - GFF2465 0.79 +0.8 85,855 + GFF2465 0.88 -0.7 85,868 - +9.0 85,876 + -0.0 85,891 + +0.3 85,941 - +0.5
Or see this region's nucleotide sequence