Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2285 and GFF2286 are separated by 262 nucleotides GFF2286 and GFF2287 are separated by 10 nucleotides GFF2287 and GFF2288 are separated by 9 nucleotides
GFF2285 - CDP-diacylglycerol pyrophosphatase (EC 3.6.1.26), at 73,510 to 74,265
GFF2285
GFF2286 - Melibiose carrier protein, Na+/melibiose symporter, at 74,528 to 75,862
GFF2286
GFF2287 - Fructokinase (EC 2.7.1.4), at 75,873 to 76,832
GFF2287
GFF2288 - ADP-ribosylglycohydrolase (EC 3.2.-.-), at 76,842 to 77,882
GFF2288
Position (kb) 74
75
76 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 73.643 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.747 kb on + strand, within GFF2285 at 73.752 kb on + strand, within GFF2285 at 73.832 kb on - strand, within GFF2285 at 73.833 kb on + strand, within GFF2285 at 73.834 kb on - strand, within GFF2285 at 73.834 kb on - strand, within GFF2285 at 73.858 kb on - strand, within GFF2285 at 74.083 kb on - strand, within GFF2285 at 74.147 kb on + strand, within GFF2285 at 74.197 kb on - strand at 74.238 kb on + strand at 74.238 kb on + strand at 74.238 kb on + strand at 74.239 kb on - strand at 74.295 kb on - strand at 74.295 kb on - strand at 74.402 kb on + strand at 74.461 kb on + strand at 74.618 kb on + strand at 74.619 kb on - strand at 74.624 kb on + strand at 74.624 kb on + strand at 74.625 kb on - strand at 74.665 kb on - strand, within GFF2286 at 74.665 kb on - strand, within GFF2286 at 74.832 kb on - strand, within GFF2286 at 74.832 kb on - strand, within GFF2286 at 74.833 kb on + strand, within GFF2286 at 74.834 kb on - strand, within GFF2286 at 74.856 kb on - strand, within GFF2286 at 74.856 kb on - strand, within GFF2286 at 74.864 kb on + strand, within GFF2286 at 74.865 kb on - strand, within GFF2286 at 74.865 kb on - strand, within GFF2286 at 74.867 kb on - strand, within GFF2286 at 74.873 kb on - strand, within GFF2286 at 74.873 kb on - strand, within GFF2286 at 74.885 kb on + strand, within GFF2286 at 74.885 kb on + strand, within GFF2286 at 75.109 kb on + strand, within GFF2286 at 75.284 kb on - strand, within GFF2286 at 75.413 kb on + strand, within GFF2286 at 75.508 kb on + strand, within GFF2286 at 75.819 kb on - strand at 76.173 kb on + strand, within GFF2287 at 76.174 kb on - strand, within GFF2287 at 76.174 kb on - strand, within GFF2287 at 76.196 kb on + strand, within GFF2287 at 76.355 kb on - strand, within GFF2287 at 76.424 kb on + strand, within GFF2287 at 76.559 kb on + strand, within GFF2287 at 76.610 kb on + strand, within GFF2287 at 76.610 kb on + strand, within GFF2287 at 76.762 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 73,643 + GFF2285 0.18 -1.2 73,733 + GFF2285 0.29 +0.2 73,733 + GFF2285 0.29 +0.1 73,733 + GFF2285 0.29 -0.5 73,747 + GFF2285 0.31 +1.2 73,752 + GFF2285 0.32 +1.2 73,832 - GFF2285 0.43 -1.0 73,833 + GFF2285 0.43 -0.5 73,834 - GFF2285 0.43 +1.7 73,834 - GFF2285 0.43 +0.3 73,858 - GFF2285 0.46 +0.0 74,083 - GFF2285 0.76 -0.3 74,147 + GFF2285 0.84 +0.2 74,197 - -0.9 74,238 + +5.5 74,238 + +0.9 74,238 + -0.5 74,239 - +2.7 74,295 - -0.2 74,295 - -0.8 74,402 + +0.1 74,461 + -0.3 74,618 + -0.3 74,619 - -0.5 74,624 + +5.9 74,624 + -0.8 74,625 - +0.2 74,665 - GFF2286 0.10 +3.2 74,665 - GFF2286 0.10 +1.4 74,832 - GFF2286 0.23 -0.3 74,832 - GFF2286 0.23 +1.9 74,833 + GFF2286 0.23 -0.5 74,834 - GFF2286 0.23 -1.5 74,856 - GFF2286 0.25 +1.1 74,856 - GFF2286 0.25 -0.2 74,864 + GFF2286 0.25 -0.5 74,865 - GFF2286 0.25 +0.1 74,865 - GFF2286 0.25 +0.1 74,867 - GFF2286 0.25 +0.5 74,873 - GFF2286 0.26 +0.0 74,873 - GFF2286 0.26 -0.3 74,885 + GFF2286 0.27 -0.4 74,885 + GFF2286 0.27 +1.4 75,109 + GFF2286 0.44 -0.6 75,284 - GFF2286 0.57 -0.1 75,413 + GFF2286 0.66 +0.3 75,508 + GFF2286 0.73 +0.3 75,819 - -1.3 76,173 + GFF2287 0.31 -0.5 76,174 - GFF2287 0.31 -0.3 76,174 - GFF2287 0.31 +0.3 76,196 + GFF2287 0.34 +1.6 76,355 - GFF2287 0.50 -0.0 76,424 + GFF2287 0.57 +0.1 76,559 + GFF2287 0.71 -0.5 76,610 + GFF2287 0.77 +0.6 76,610 + GFF2287 0.77 -0.1 76,762 + -0.4
Or see this region's nucleotide sequence