Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2226

Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2224 and GFF2225 overlap by 16 nucleotidesGFF2225 and GFF2226 overlap by 4 nucleotidesGFF2226 and GFF2227 are separated by 1 nucleotidesGFF2227 and GFF2228 overlap by 8 nucleotides GFF2224 - Putative hydrolase, at 18,186 to 18,920 GFF2224 GFF2225 - FIG01046022: hypothetical protein, at 18,905 to 19,783 GFF2225 GFF2226 - Hypothetical radical SAM family enzyme in interesting gene cluster, at 19,780 to 21,021 GFF2226 GFF2227 - FIG01124638: hypothetical protein, at 21,023 to 21,898 GFF2227 GFF2228 - FIG00638667: hypothetical protein, at 21,891 to 22,877 GFF2228 Position (kb) 19 20 21 22Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7at 18.906 kb on + strandat 18.991 kb on - strandat 19.500 kb on - strand, within GFF2225at 19.560 kb on + strand, within GFF2225at 19.561 kb on - strand, within GFF2225at 19.563 kb on + strand, within GFF2225at 19.594 kb on + strand, within GFF2225at 19.595 kb on - strand, within GFF2225at 19.705 kb on - strandat 19.716 kb on + strandat 19.716 kb on + strandat 19.717 kb on - strandat 19.729 kb on + strandat 19.729 kb on + strandat 19.730 kb on - strandat 19.898 kb on + strandat 19.899 kb on - strandat 20.141 kb on + strand, within GFF2226at 20.304 kb on - strand, within GFF2226at 20.307 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.338 kb on - strand, within GFF2226at 20.526 kb on + strand, within GFF2226at 20.527 kb on - strand, within GFF2226at 20.527 kb on - strand, within GFF2226at 20.531 kb on - strand, within GFF2226at 20.562 kb on + strand, within GFF2226at 20.716 kb on + strand, within GFF2226at 20.807 kb on + strand, within GFF2226at 20.886 kb on + strand, within GFF2226at 20.895 kb on - strand, within GFF2226at 20.895 kb on - strand, within GFF2226at 20.973 kb on + strandat 20.992 kb on - strandat 20.994 kb on - strandat 21.015 kb on + strandat 21.022 kb on - strandat 21.054 kb on + strandat 21.096 kb on + strandat 21.096 kb on + strandat 21.207 kb on + strand, within GFF2227at 21.207 kb on + strand, within GFF2227at 21.208 kb on - strand, within GFF2227at 21.303 kb on + strand, within GFF2227at 21.304 kb on - strand, within GFF2227at 21.388 kb on + strand, within GFF2227at 21.485 kb on - strand, within GFF2227at 21.625 kb on + strand, within GFF2227at 21.712 kb on - strand, within GFF2227at 21.787 kb on + strand, within GFF2227at 21.812 kb on - strandat 22.016 kb on + strand, within GFF2228

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI
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18,906 + +0.5
18,991 - -0.0
19,500 - GFF2225 0.68 -0.4
19,560 + GFF2225 0.75 +1.0
19,561 - GFF2225 0.75 +0.1
19,563 + GFF2225 0.75 -1.5
19,594 + GFF2225 0.78 +0.7
19,595 - GFF2225 0.78 +1.1
19,705 - +1.2
19,716 + -0.4
19,716 + -1.4
19,717 - +0.4
19,729 + +1.6
19,729 + -1.1
19,730 - +0.9
19,898 + +0.7
19,899 - +0.2
20,141 + GFF2226 0.29 -1.1
20,304 - GFF2226 0.42 +0.1
20,307 - GFF2226 0.42 +1.5
20,311 - GFF2226 0.43 +0.2
20,311 - GFF2226 0.43 +0.6
20,311 - GFF2226 0.43 +0.4
20,338 - GFF2226 0.45 +0.4
20,526 + GFF2226 0.60 -1.7
20,527 - GFF2226 0.60 +0.2
20,527 - GFF2226 0.60 +1.8
20,531 - GFF2226 0.60 +1.3
20,562 + GFF2226 0.63 +0.5
20,716 + GFF2226 0.75 +0.5
20,807 + GFF2226 0.83 +0.5
20,886 + GFF2226 0.89 +0.5
20,895 - GFF2226 0.90 +0.6
20,895 - GFF2226 0.90 -0.3
20,973 + +0.7
20,992 - +0.5
20,994 - +0.7
21,015 + -0.3
21,022 - +0.0
21,054 + -0.2
21,096 + -1.0
21,096 + +0.1
21,207 + GFF2227 0.21 -0.0
21,207 + GFF2227 0.21 -0.2
21,208 - GFF2227 0.21 -1.5
21,303 + GFF2227 0.32 +0.1
21,304 - GFF2227 0.32 +4.2
21,388 + GFF2227 0.42 -1.7
21,485 - GFF2227 0.53 +0.1
21,625 + GFF2227 0.69 -0.2
21,712 - GFF2227 0.79 -0.1
21,787 + GFF2227 0.87 -0.4
21,812 - +7.2
22,016 + GFF2228 0.13 -0.0

Or see this region's nucleotide sequence