Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2125 and GFF2126 are separated by 26 nucleotides GFF2126 and GFF2127 overlap by 11 nucleotides GFF2127 and GFF2128 are separated by 108 nucleotides
GFF2125 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 12,309 to 13,175
GFF2125
GFF2126 - Pantothenate:Na+ symporter (TC 2.A.21.1.1), at 13,202 to 14,653
GFF2126
GFF2127 - FIG003021: Membrane protein, at 14,643 to 14,885
GFF2127
GFF2128 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 14,994 to 16,343
GFF2128
Position (kb) 13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 12.310 kb on + strand at 12.419 kb on + strand, within GFF2125 at 12.420 kb on - strand, within GFF2125 at 12.504 kb on - strand, within GFF2125 at 12.553 kb on + strand, within GFF2125 at 12.612 kb on - strand, within GFF2125 at 12.751 kb on - strand, within GFF2125 at 12.887 kb on + strand, within GFF2125 at 13.317 kb on + strand at 13.328 kb on - strand at 13.328 kb on - strand at 13.348 kb on - strand, within GFF2126 at 13.349 kb on + strand, within GFF2126 at 13.349 kb on + strand, within GFF2126 at 13.529 kb on + strand, within GFF2126 at 13.534 kb on + strand, within GFF2126 at 13.535 kb on - strand, within GFF2126 at 13.549 kb on + strand, within GFF2126 at 13.550 kb on - strand, within GFF2126 at 13.550 kb on - strand, within GFF2126 at 13.550 kb on - strand, within GFF2126 at 13.826 kb on - strand, within GFF2126 at 13.832 kb on - strand, within GFF2126 at 13.834 kb on + strand, within GFF2126 at 13.835 kb on - strand, within GFF2126 at 13.855 kb on + strand, within GFF2126 at 13.865 kb on - strand, within GFF2126 at 13.939 kb on + strand, within GFF2126 at 13.955 kb on + strand, within GFF2126 at 13.956 kb on - strand, within GFF2126 at 13.956 kb on - strand, within GFF2126 at 14.138 kb on - strand, within GFF2126 at 14.139 kb on + strand, within GFF2126 at 14.439 kb on + strand, within GFF2126 at 14.439 kb on + strand, within GFF2126 at 14.443 kb on + strand, within GFF2126 at 14.444 kb on - strand, within GFF2126 at 14.445 kb on + strand, within GFF2126 at 14.486 kb on - strand, within GFF2126 at 14.487 kb on + strand, within GFF2126 at 14.487 kb on + strand, within GFF2126 at 14.487 kb on + strand, within GFF2126 at 14.487 kb on + strand, within GFF2126 at 14.487 kb on + strand, within GFF2126 at 14.488 kb on - strand, within GFF2126 at 14.547 kb on + strand at 14.573 kb on - strand at 14.620 kb on - strand at 14.624 kb on + strand at 14.794 kb on + strand, within GFF2127 at 14.822 kb on - strand, within GFF2127 at 14.822 kb on - strand, within GFF2127 at 14.921 kb on + strand at 14.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 12,310 + -0.8 12,419 + GFF2125 0.13 +0.8 12,420 - GFF2125 0.13 -0.8 12,504 - GFF2125 0.22 +0.6 12,553 + GFF2125 0.28 +0.5 12,612 - GFF2125 0.35 +1.8 12,751 - GFF2125 0.51 -1.0 12,887 + GFF2125 0.67 +0.3 13,317 + -0.5 13,328 - -0.3 13,328 - -0.4 13,348 - GFF2126 0.10 +0.5 13,349 + GFF2126 0.10 +0.3 13,349 + GFF2126 0.10 +0.2 13,529 + GFF2126 0.23 +0.4 13,534 + GFF2126 0.23 -0.6 13,535 - GFF2126 0.23 -1.2 13,549 + GFF2126 0.24 -0.3 13,550 - GFF2126 0.24 +0.5 13,550 - GFF2126 0.24 -0.3 13,550 - GFF2126 0.24 +0.6 13,826 - GFF2126 0.43 -0.2 13,832 - GFF2126 0.43 +0.2 13,834 + GFF2126 0.44 -0.6 13,835 - GFF2126 0.44 +1.3 13,855 + GFF2126 0.45 +0.4 13,865 - GFF2126 0.46 -2.8 13,939 + GFF2126 0.51 +0.1 13,955 + GFF2126 0.52 +0.4 13,956 - GFF2126 0.52 -0.5 13,956 - GFF2126 0.52 +1.2 14,138 - GFF2126 0.64 +0.3 14,139 + GFF2126 0.65 -0.0 14,439 + GFF2126 0.85 -0.5 14,439 + GFF2126 0.85 +0.8 14,443 + GFF2126 0.85 +0.3 14,444 - GFF2126 0.86 -0.7 14,445 + GFF2126 0.86 -0.4 14,486 - GFF2126 0.88 -0.0 14,487 + GFF2126 0.88 +0.8 14,487 + GFF2126 0.88 +0.5 14,487 + GFF2126 0.88 +0.2 14,487 + GFF2126 0.88 +0.3 14,487 + GFF2126 0.88 +5.8 14,488 - GFF2126 0.89 +0.3 14,547 + +0.7 14,573 - -2.1 14,620 - -1.2 14,624 + +0.8 14,794 + GFF2127 0.62 -1.5 14,822 - GFF2127 0.74 -0.0 14,822 - GFF2127 0.74 -0.3 14,921 + -0.2 14,922 - -0.7
Or see this region's nucleotide sequence