Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2076 and GFF2077 overlap by 11 nucleotides GFF2077 and GFF2078 are separated by 10 nucleotides GFF2078 and GFF2079 are separated by 11 nucleotides GFF2079 and GFF2080 are separated by 100 nucleotides GFF2080 and GFF2081 are separated by 40 nucleotides
GFF2076 - Ethanolamine utilization protein EutG, at 39,389 to 40,576
GFF2076
GFF2077 - Ethanolamine utilization protein EutJ, at 40,566 to 41,405
GFF2077
GFF2078 - Acetaldehyde dehydrogenase, ethanolamine utilization cluster (EC 1.2.1.10), at 41,416 to 42,819
GFF2078
GFF2079 - Ethanolamine utilization polyhedral-body-like protein EutN, at 42,831 to 43,130
GFF2079
GFF2080 - Ethanolamine utilization polyhedral-body-like protein EutM, at 43,231 to 43,521
GFF2080
GFF2081 - Phosphate acetyltransferase (EC 2.3.1.8), ethanolamine utilization-specific, at 43,562 to 44,578
GFF2081
Position (kb) 41
42
43 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 40.431 kb on - strand, within GFF2076 at 40.562 kb on + strand at 40.562 kb on + strand at 40.563 kb on - strand at 40.628 kb on + strand at 40.889 kb on + strand, within GFF2077 at 40.890 kb on - strand, within GFF2077 at 40.921 kb on + strand, within GFF2077 at 40.922 kb on - strand, within GFF2077 at 40.922 kb on - strand, within GFF2077 at 40.922 kb on - strand, within GFF2077 at 40.922 kb on - strand, within GFF2077 at 41.034 kb on + strand, within GFF2077 at 41.508 kb on + strand at 41.508 kb on + strand at 42.139 kb on - strand, within GFF2078 at 42.139 kb on - strand, within GFF2078 at 42.833 kb on - strand at 42.833 kb on - strand at 42.837 kb on - strand at 43.086 kb on + strand, within GFF2079 at 43.086 kb on + strand, within GFF2079 at 43.398 kb on + strand, within GFF2080 at 43.398 kb on + strand, within GFF2080 at 43.454 kb on - strand, within GFF2080 at 43.580 kb on + strand at 43.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 40,431 - GFF2076 0.88 +0.4 40,562 + -0.9 40,562 + -0.6 40,563 - -0.3 40,628 + +1.3 40,889 + GFF2077 0.38 +0.5 40,890 - GFF2077 0.39 -1.0 40,921 + GFF2077 0.42 +0.0 40,922 - GFF2077 0.42 +0.5 40,922 - GFF2077 0.42 +0.4 40,922 - GFF2077 0.42 -0.1 40,922 - GFF2077 0.42 -1.0 41,034 + GFF2077 0.56 +1.7 41,508 + -0.4 41,508 + -1.3 42,139 - GFF2078 0.51 +3.9 42,139 - GFF2078 0.51 +0.6 42,833 - -0.3 42,833 - +0.6 42,837 - +0.4 43,086 + GFF2079 0.85 +0.5 43,086 + GFF2079 0.85 +6.9 43,398 + GFF2080 0.57 -0.5 43,398 + GFF2080 0.57 +0.7 43,454 - GFF2080 0.77 -0.7 43,580 + +0.5 43,643 - -0.2
Or see this region's nucleotide sequence