Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1988

Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1988 and GFF1989 are separated by 61 nucleotidesGFF1989 and GFF1990 are separated by 106 nucleotides GFF1988 - Arginine ABC transporter, periplasmic arginine-binding protein ArtI, at 330 to 1,070 GFF1988 GFF1989 - '3'-to-5' oligoribonuclease (orn)' transl_table=11, at 1,132 to 1,677 GFF1989 GFF1990 - Ribosome small subunit-stimulated GTPase EngC, at 1,784 to 2,836 GFF1990 Position (kb) 0 1 2Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 0.264 kb on + strandat 0.264 kb on + strandat 0.426 kb on + strand, within GFF1988at 0.427 kb on - strand, within GFF1988at 0.545 kb on + strand, within GFF1988at 0.546 kb on - strand, within GFF1988at 0.562 kb on + strand, within GFF1988at 0.562 kb on + strand, within GFF1988at 0.602 kb on + strand, within GFF1988at 0.603 kb on - strand, within GFF1988at 0.646 kb on - strand, within GFF1988at 0.648 kb on + strand, within GFF1988at 0.681 kb on - strand, within GFF1988at 0.681 kb on - strand, within GFF1988at 0.690 kb on + strand, within GFF1988at 0.691 kb on - strand, within GFF1988at 0.691 kb on - strand, within GFF1988at 0.952 kb on + strand, within GFF1988at 0.998 kb on + strandat 1.061 kb on + strandat 1.126 kb on + strandat 1.718 kb on + strandat 1.719 kb on - strandat 1.727 kb on - strandat 1.727 kb on - strandat 1.738 kb on + strandat 1.739 kb on - strandat 1.739 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI
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264 + +0.4
264 + -0.7
426 + GFF1988 0.13 -1.3
427 - GFF1988 0.13 -1.2
545 + GFF1988 0.29 +1.7
546 - GFF1988 0.29 -0.8
562 + GFF1988 0.31 +5.8
562 + GFF1988 0.31 +0.4
602 + GFF1988 0.37 -0.4
603 - GFF1988 0.37 +1.6
646 - GFF1988 0.43 -0.9
648 + GFF1988 0.43 +0.4
681 - GFF1988 0.47 -0.2
681 - GFF1988 0.47 +5.4
690 + GFF1988 0.49 -0.7
691 - GFF1988 0.49 +0.3
691 - GFF1988 0.49 -0.0
952 + GFF1988 0.84 +0.3
998 + +1.4
1,061 + +0.0
1,126 + +0.2
1,718 + +3.1
1,719 - +1.0
1,727 - -0.8
1,727 - -0.6
1,738 + +1.0
1,739 - +0.6
1,739 - +0.7

Or see this region's nucleotide sequence