Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1737 and GFF1738 are separated by 14 nucleotides GFF1738 and GFF1739 are separated by 94 nucleotides GFF1739 and GFF1740 are separated by 168 nucleotides
GFF1737 - Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1), at 38,290 to 39,099
GFF1737
GFF1738 - Phosphate transport system regulatory protein PhoU, at 39,114 to 39,839
GFF1738
GFF1739 - Putative phosphatase YieH, at 39,934 to 40,599
GFF1739
GFF1740 - Xanthine/uracil/thiamine/ascorbate permease family protein, at 40,768 to 42,105
GFF1740
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 38.997 kb on + strand, within GFF1737 at 39.001 kb on + strand, within GFF1737 at 39.806 kb on + strand at 39.880 kb on + strand at 39.886 kb on + strand at 39.935 kb on + strand at 39.942 kb on + strand at 39.993 kb on + strand at 40.029 kb on + strand, within GFF1739 at 40.239 kb on + strand, within GFF1739 at 40.300 kb on - strand, within GFF1739 at 40.300 kb on - strand, within GFF1739 at 40.300 kb on - strand, within GFF1739 at 40.454 kb on - strand, within GFF1739 at 40.454 kb on - strand, within GFF1739 at 40.474 kb on + strand, within GFF1739 at 40.474 kb on + strand, within GFF1739 at 40.475 kb on - strand, within GFF1739 at 40.494 kb on + strand, within GFF1739 at 40.495 kb on - strand, within GFF1739 at 40.515 kb on + strand, within GFF1739 at 40.515 kb on + strand, within GFF1739 at 40.630 kb on - strand at 40.725 kb on + strand at 40.807 kb on - strand at 40.824 kb on + strand at 40.898 kb on - strand at 40.932 kb on - strand, within GFF1740 at 41.151 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.439 kb on - strand, within GFF1740 at 41.443 kb on + strand, within GFF1740 at 41.443 kb on + strand, within GFF1740 at 41.444 kb on - strand, within GFF1740 at 41.452 kb on - strand, within GFF1740
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 38,997 + GFF1737 0.87 +2.9 39,001 + GFF1737 0.88 -0.1 39,806 + -0.1 39,880 + +0.9 39,886 + -0.7 39,935 + -0.7 39,942 + +0.1 39,993 + -0.4 40,029 + GFF1739 0.14 -0.2 40,239 + GFF1739 0.46 +7.8 40,300 - GFF1739 0.55 +0.9 40,300 - GFF1739 0.55 -0.4 40,300 - GFF1739 0.55 +1.3 40,454 - GFF1739 0.78 -1.4 40,454 - GFF1739 0.78 +0.4 40,474 + GFF1739 0.81 -0.4 40,474 + GFF1739 0.81 +1.6 40,475 - GFF1739 0.81 -0.5 40,494 + GFF1739 0.84 +6.9 40,495 - GFF1739 0.84 +1.0 40,515 + GFF1739 0.87 +0.7 40,515 + GFF1739 0.87 +1.0 40,630 - -2.1 40,725 + -0.5 40,807 - +8.4 40,824 + +0.0 40,898 - +0.1 40,932 - GFF1740 0.12 +3.8 41,151 + GFF1740 0.29 +0.9 41,438 + GFF1740 0.50 +0.2 41,438 + GFF1740 0.50 -1.4 41,438 + GFF1740 0.50 +0.4 41,439 - GFF1740 0.50 +0.9 41,443 + GFF1740 0.50 +0.6 41,443 + GFF1740 0.50 +0.3 41,444 - GFF1740 0.51 -0.7 41,452 - GFF1740 0.51 +0.0
Or see this region's nucleotide sequence