Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1732

Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1731 and GFF1732 are separated by 61 nucleotidesGFF1732 and GFF1733 are separated by 48 nucleotides GFF1731 - SgrR, sugar-phosphate stress, transcriptional activator of SgrS small RNA, at 30,955 to 32,745 GFF1731 GFF1732 - Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25), at 32,807 to 33,625 GFF1732 GFF1733 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 33,674 to 35,047 GFF1733 Position (kb) 32 33 34Strain fitness (log2 ratio) -1 0 1at 31.975 kb on - strand, within GFF1731at 32.239 kb on + strand, within GFF1731at 32.239 kb on + strand, within GFF1731at 32.300 kb on - strand, within GFF1731at 32.372 kb on + strand, within GFF1731at 32.372 kb on + strand, within GFF1731at 32.413 kb on - strand, within GFF1731at 32.413 kb on - strand, within GFF1731at 32.482 kb on + strand, within GFF1731at 32.483 kb on - strand, within GFF1731at 32.499 kb on + strand, within GFF1731at 32.595 kb on + strandat 32.635 kb on + strandat 32.635 kb on + strandat 32.635 kb on + strandat 32.635 kb on + strandat 32.674 kb on + strandat 32.680 kb on + strandat 32.680 kb on + strandat 32.681 kb on - strandat 32.891 kb on + strand, within GFF1732at 32.891 kb on + strand, within GFF1732at 32.919 kb on - strand, within GFF1732at 32.919 kb on - strand, within GFF1732at 32.925 kb on - strand, within GFF1732at 33.077 kb on - strand, within GFF1732at 33.077 kb on - strand, within GFF1732at 33.077 kb on - strand, within GFF1732at 33.091 kb on + strand, within GFF1732at 33.125 kb on + strand, within GFF1732at 33.156 kb on - strand, within GFF1732at 33.203 kb on - strand, within GFF1732at 33.257 kb on - strand, within GFF1732at 33.408 kb on - strand, within GFF1732at 33.439 kb on + strand, within GFF1732at 33.536 kb on - strand, within GFF1732at 33.536 kb on - strand, within GFF1732at 33.563 kb on + strandat 33.673 kb on + strandat 33.731 kb on - strandat 33.912 kb on - strand, within GFF1733at 33.987 kb on - strand, within GFF1733at 34.045 kb on + strand, within GFF1733at 34.046 kb on - strand, within GFF1733at 34.054 kb on - strand, within GFF1733at 34.072 kb on + strand, within GFF1733at 34.171 kb on - strand, within GFF1733at 34.186 kb on + strand, within GFF1733at 34.187 kb on - strand, within GFF1733at 34.187 kb on - strand, within GFF1733at 34.229 kb on - strand, within GFF1733at 34.402 kb on + strand, within GFF1733at 34.449 kb on + strand, within GFF1733at 34.449 kb on + strand, within GFF1733at 34.537 kb on + strand, within GFF1733at 34.537 kb on + strand, within GFF1733at 34.598 kb on - strand, within GFF1733at 34.610 kb on - strand, within GFF1733

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI
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31,975 - GFF1731 0.57 -1.7
32,239 + GFF1731 0.72 -0.0
32,239 + GFF1731 0.72 +0.6
32,300 - GFF1731 0.75 -0.5
32,372 + GFF1731 0.79 -0.4
32,372 + GFF1731 0.79 +0.0
32,413 - GFF1731 0.81 +0.6
32,413 - GFF1731 0.81 -0.2
32,482 + GFF1731 0.85 +0.0
32,483 - GFF1731 0.85 -1.1
32,499 + GFF1731 0.86 -0.2
32,595 + +1.0
32,635 + +0.2
32,635 + -0.1
32,635 + +0.3
32,635 + -0.1
32,674 + +0.6
32,680 + +1.3
32,680 + -0.2
32,681 - -0.0
32,891 + GFF1732 0.10 -1.2
32,891 + GFF1732 0.10 -0.3
32,919 - GFF1732 0.14 +1.4
32,919 - GFF1732 0.14 +0.0
32,925 - GFF1732 0.14 +0.5
33,077 - GFF1732 0.33 +0.4
33,077 - GFF1732 0.33 +0.2
33,077 - GFF1732 0.33 -0.3
33,091 + GFF1732 0.35 -1.0
33,125 + GFF1732 0.39 -1.4
33,156 - GFF1732 0.43 -0.7
33,203 - GFF1732 0.48 +1.5
33,257 - GFF1732 0.55 -1.0
33,408 - GFF1732 0.73 -0.0
33,439 + GFF1732 0.77 +1.7
33,536 - GFF1732 0.89 +0.8
33,536 - GFF1732 0.89 -1.1
33,563 + -0.9
33,673 + -0.4
33,731 - -0.1
33,912 - GFF1733 0.17 +0.5
33,987 - GFF1733 0.23 +0.2
34,045 + GFF1733 0.27 +0.3
34,046 - GFF1733 0.27 +0.0
34,054 - GFF1733 0.28 +1.0
34,072 + GFF1733 0.29 -0.7
34,171 - GFF1733 0.36 +0.7
34,186 + GFF1733 0.37 -0.5
34,187 - GFF1733 0.37 -0.3
34,187 - GFF1733 0.37 +0.8
34,229 - GFF1733 0.40 +0.2
34,402 + GFF1733 0.53 +1.3
34,449 + GFF1733 0.56 +1.2
34,449 + GFF1733 0.56 +0.3
34,537 + GFF1733 0.63 -0.7
34,537 + GFF1733 0.63 -1.5
34,598 - GFF1733 0.67 -1.5
34,610 - GFF1733 0.68 +1.1

Or see this region's nucleotide sequence