Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1470 and GFF1471 are separated by 133 nucleotides GFF1471 and GFF1472 are separated by 208 nucleotides
GFF1470 - Phosphoethanolamine transferase EptA specific for the 1 phosphate group of core-lipid A, at 134,515 to 136,158
GFF1470
GFF1471 - Arginine/agmatine antiporter, at 136,292 to 137,560
GFF1471
GFF1472 - Transcriptional regulator of catabolic arginine decarboxylase (adiA), at 137,769 to 138,500
GFF1472
Position (kb) 136
137
138 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 135.348 kb on - strand, within GFF1470 at 135.509 kb on - strand, within GFF1470 at 135.542 kb on + strand, within GFF1470 at 135.542 kb on + strand, within GFF1470 at 135.564 kb on + strand, within GFF1470 at 135.565 kb on - strand, within GFF1470 at 135.624 kb on - strand, within GFF1470 at 135.702 kb on + strand, within GFF1470 at 135.706 kb on + strand, within GFF1470 at 135.707 kb on - strand, within GFF1470 at 135.970 kb on - strand, within GFF1470 at 135.982 kb on - strand, within GFF1470 at 136.033 kb on + strand at 136.040 kb on - strand at 136.040 kb on - strand at 136.049 kb on - strand at 136.052 kb on - strand at 136.052 kb on - strand at 136.061 kb on + strand at 136.061 kb on + strand at 136.089 kb on + strand at 136.090 kb on - strand at 136.091 kb on + strand at 136.092 kb on - strand at 136.153 kb on + strand at 136.154 kb on - strand at 136.219 kb on - strand at 136.227 kb on - strand at 136.279 kb on + strand at 136.279 kb on + strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.280 kb on - strand at 136.284 kb on - strand at 136.324 kb on - strand at 136.347 kb on + strand at 136.348 kb on - strand at 136.359 kb on + strand at 136.360 kb on - strand at 136.441 kb on - strand, within GFF1471 at 136.459 kb on + strand, within GFF1471 at 136.511 kb on + strand, within GFF1471 at 136.828 kb on + strand, within GFF1471 at 136.915 kb on - strand, within GFF1471 at 137.096 kb on - strand, within GFF1471 at 137.249 kb on - strand, within GFF1471 at 137.258 kb on + strand, within GFF1471 at 137.258 kb on + strand, within GFF1471 at 137.299 kb on - strand, within GFF1471 at 137.409 kb on + strand, within GFF1471 at 137.409 kb on + strand, within GFF1471 at 137.410 kb on - strand, within GFF1471 at 137.410 kb on - strand, within GFF1471 at 137.410 kb on - strand, within GFF1471 at 137.432 kb on + strand, within GFF1471 at 137.669 kb on + strand at 137.676 kb on + strand at 137.676 kb on + strand at 137.706 kb on - strand at 137.736 kb on + strand at 137.736 kb on + strand at 137.804 kb on - strand at 137.822 kb on - strand at 137.866 kb on + strand, within GFF1472 at 138.342 kb on - strand, within GFF1472 at 138.428 kb on + strand at 138.459 kb on - strand at 138.465 kb on + strand at 138.501 kb on + strand at 138.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 135,348 - GFF1470 0.51 -0.3 135,509 - GFF1470 0.60 +0.2 135,542 + GFF1470 0.62 +1.0 135,542 + GFF1470 0.62 +0.8 135,564 + GFF1470 0.64 -1.2 135,565 - GFF1470 0.64 +0.2 135,624 - GFF1470 0.67 +1.0 135,702 + GFF1470 0.72 +0.2 135,706 + GFF1470 0.72 +1.4 135,707 - GFF1470 0.73 -1.5 135,970 - GFF1470 0.89 +8.0 135,982 - GFF1470 0.89 +0.4 136,033 + +0.3 136,040 - +0.4 136,040 - -0.2 136,049 - +2.1 136,052 - +1.0 136,052 - -0.6 136,061 + +0.3 136,061 + -0.5 136,089 + +0.0 136,090 - +0.8 136,091 + +0.1 136,092 - -0.4 136,153 + +0.1 136,154 - +0.5 136,219 - +0.5 136,227 - +0.0 136,279 + -0.2 136,279 + -0.5 136,280 - +0.4 136,280 - -1.6 136,280 - +0.5 136,280 - -0.5 136,280 - +1.1 136,280 - -0.1 136,284 - +0.2 136,324 - +0.0 136,347 + -0.6 136,348 - +8.7 136,359 + +0.5 136,360 - -0.1 136,441 - GFF1471 0.12 -0.5 136,459 + GFF1471 0.13 +0.4 136,511 + GFF1471 0.17 -0.2 136,828 + GFF1471 0.42 +0.4 136,915 - GFF1471 0.49 +0.3 137,096 - GFF1471 0.63 -0.6 137,249 - GFF1471 0.75 -0.3 137,258 + GFF1471 0.76 -0.1 137,258 + GFF1471 0.76 +0.4 137,299 - GFF1471 0.79 +0.4 137,409 + GFF1471 0.88 +0.6 137,409 + GFF1471 0.88 +0.6 137,410 - GFF1471 0.88 +0.3 137,410 - GFF1471 0.88 -0.2 137,410 - GFF1471 0.88 -0.8 137,432 + GFF1471 0.90 +0.6 137,669 + -1.9 137,676 + +0.2 137,676 + +0.6 137,706 - -1.9 137,736 + -0.2 137,736 + +0.6 137,804 - -0.2 137,822 - +1.3 137,866 + GFF1472 0.13 +0.1 138,342 - GFF1472 0.78 +0.3 138,428 + +0.1 138,459 - +0.4 138,465 + +0.2 138,501 + +0.1 138,555 + -0.9
Or see this region's nucleotide sequence