Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1354 and GFF1355 overlap by 37 nucleotides GFF1355 and GFF1356 overlap by 17 nucleotides GFF1356 and GFF1357 are separated by 123 nucleotides GFF1357 and GFF1358 are separated by 48 nucleotides
GFF1354 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 5,206 to 6,957
GFF1354
GFF1355 - hypothetical protein, at 6,921 to 7,355
GFF1355
GFF1356 - Acetate operon repressor, at 7,339 to 8,163
GFF1356
GFF1357 - hypothetical protein, at 8,287 to 8,418
GFF1357
GFF1358 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), at 8,467 to 12,150
GFF1358
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-1
0
1
2 at 6.426 kb on + strand, within GFF1354 at 6.427 kb on - strand, within GFF1354 at 6.447 kb on + strand, within GFF1354 at 6.448 kb on - strand, within GFF1354 at 6.526 kb on + strand, within GFF1354 at 6.576 kb on + strand, within GFF1354 at 6.625 kb on + strand, within GFF1354 at 6.632 kb on - strand, within GFF1354 at 6.647 kb on - strand, within GFF1354 at 6.723 kb on + strand, within GFF1354 at 6.724 kb on - strand, within GFF1354 at 6.875 kb on + strand at 6.876 kb on - strand at 6.876 kb on - strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.886 kb on + strand at 6.887 kb on - strand at 6.887 kb on - strand at 6.887 kb on - strand at 6.913 kb on - strand at 6.939 kb on + strand at 6.940 kb on - strand at 7.082 kb on + strand, within GFF1355 at 7.156 kb on + strand, within GFF1355 at 7.196 kb on + strand, within GFF1355 at 7.208 kb on + strand, within GFF1355 at 7.355 kb on + strand at 7.355 kb on + strand at 7.485 kb on + strand, within GFF1356 at 7.605 kb on + strand, within GFF1356 at 8.214 kb on - strand at 8.310 kb on + strand, within GFF1357 at 8.541 kb on + strand at 8.541 kb on + strand at 8.554 kb on + strand at 8.561 kb on + strand at 8.677 kb on + strand at 8.719 kb on - strand at 8.780 kb on - strand at 8.922 kb on + strand, within GFF1358 at 8.926 kb on - strand, within GFF1358 at 9.160 kb on + strand, within GFF1358 at 9.161 kb on - strand, within GFF1358 at 9.163 kb on - strand, within GFF1358
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 6,426 + GFF1354 0.70 -0.1 6,427 - GFF1354 0.70 -0.3 6,447 + GFF1354 0.71 -1.0 6,448 - GFF1354 0.71 +0.8 6,526 + GFF1354 0.75 +0.2 6,576 + GFF1354 0.78 -0.2 6,625 + GFF1354 0.81 +1.0 6,632 - GFF1354 0.81 -0.2 6,647 - GFF1354 0.82 -0.4 6,723 + GFF1354 0.87 -1.2 6,724 - GFF1354 0.87 +0.8 6,875 + -0.2 6,876 - +1.7 6,876 - -1.2 6,886 + -0.1 6,886 + +1.0 6,886 + -1.0 6,886 + -0.3 6,887 - -1.4 6,887 - -0.9 6,887 - -0.3 6,913 - +0.2 6,939 + +0.3 6,940 - +2.3 7,082 + GFF1355 0.37 +1.1 7,156 + GFF1355 0.54 +1.6 7,196 + GFF1355 0.63 +0.2 7,208 + GFF1355 0.66 -0.5 7,355 + -0.5 7,355 + -0.9 7,485 + GFF1356 0.18 +0.4 7,605 + GFF1356 0.32 +0.8 8,214 - +0.3 8,310 + GFF1357 0.17 -1.2 8,541 + +0.0 8,541 + -0.7 8,554 + +0.1 8,561 + +0.1 8,677 + -0.2 8,719 - -0.4 8,780 - -1.4 8,922 + GFF1358 0.12 -0.9 8,926 - GFF1358 0.12 +0.1 9,160 + GFF1358 0.19 -0.4 9,161 - GFF1358 0.19 -1.0 9,163 - GFF1358 0.19 +0.1
Or see this region's nucleotide sequence