Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1289 and GFF1290 are separated by 50 nucleotides GFF1290 and GFF1291 are separated by 62 nucleotides GFF1291 and GFF1292 overlap by 29 nucleotides GFF1292 and GFF1293 are separated by 2 nucleotides GFF1293 and GFF1294 are separated by 9 nucleotides
GFF1289 - Phage tail assembly, at 13,437 to 14,180
GFF1289
GFF1290 - Phage minor tail protein, at 14,231 to 14,626
GFF1290
GFF1291 - Phage minor tail protein #Phage minor tail protein T, at 14,689 to 14,961
GFF1291
GFF1292 - Phage tail length tape-measure protein 1, at 14,933 to 18,028
GFF1292
GFF1293 - Phage minor tail protein, at 18,031 to 18,360
GFF1293
GFF1294 - Phage minor tail protein, at 18,370 to 19,068
GFF1294
Position (kb) 14
15
16
17
18
19 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9 at 14.254 kb on + strand at 14.255 kb on - strand at 14.324 kb on - strand, within GFF1290 at 14.413 kb on + strand, within GFF1290 at 14.414 kb on - strand, within GFF1290 at 14.416 kb on + strand, within GFF1290 at 14.417 kb on - strand, within GFF1290 at 14.480 kb on + strand, within GFF1290 at 14.508 kb on - strand, within GFF1290 at 14.554 kb on + strand, within GFF1290 at 14.554 kb on + strand, within GFF1290 at 14.913 kb on + strand, within GFF1291 at 14.964 kb on + strand at 14.968 kb on + strand at 15.144 kb on - strand at 15.178 kb on + strand at 15.325 kb on - strand, within GFF1292 at 15.325 kb on - strand, within GFF1292 at 15.502 kb on + strand, within GFF1292 at 15.601 kb on + strand, within GFF1292 at 15.605 kb on + strand, within GFF1292 at 15.606 kb on - strand, within GFF1292 at 15.608 kb on - strand, within GFF1292 at 15.643 kb on + strand, within GFF1292 at 15.854 kb on + strand, within GFF1292 at 15.858 kb on - strand, within GFF1292 at 15.914 kb on + strand, within GFF1292 at 15.914 kb on + strand, within GFF1292 at 16.299 kb on + strand, within GFF1292 at 16.300 kb on - strand, within GFF1292 at 16.733 kb on - strand, within GFF1292 at 17.199 kb on - strand, within GFF1292 at 17.327 kb on + strand, within GFF1292 at 17.642 kb on + strand, within GFF1292 at 17.643 kb on - strand, within GFF1292 at 17.714 kb on + strand, within GFF1292 at 17.732 kb on - strand at 17.799 kb on + strand at 17.800 kb on - strand at 17.803 kb on - strand at 17.827 kb on + strand at 17.883 kb on - strand at 17.947 kb on - strand at 17.949 kb on - strand at 17.950 kb on + strand at 17.950 kb on + strand at 17.951 kb on - strand at 18.160 kb on + strand, within GFF1293 at 18.161 kb on - strand, within GFF1293 at 18.183 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.391 kb on - strand at 18.536 kb on - strand, within GFF1294 at 18.689 kb on + strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.807 kb on - strand, within GFF1294 at 18.888 kb on + strand, within GFF1294 at 18.896 kb on + strand, within GFF1294 at 18.919 kb on + strand, within GFF1294 at 18.921 kb on + strand, within GFF1294 at 18.957 kb on + strand, within GFF1294
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 14,254 + +0.2 14,255 - +0.9 14,324 - GFF1290 0.23 +1.4 14,413 + GFF1290 0.46 -0.1 14,414 - GFF1290 0.46 -0.5 14,416 + GFF1290 0.47 -0.5 14,417 - GFF1290 0.47 +7.8 14,480 + GFF1290 0.63 -0.9 14,508 - GFF1290 0.70 +0.8 14,554 + GFF1290 0.82 +2.0 14,554 + GFF1290 0.82 -0.4 14,913 + GFF1291 0.82 -1.1 14,964 + -0.2 14,968 + +0.1 15,144 - -0.3 15,178 + +0.0 15,325 - GFF1292 0.13 +0.5 15,325 - GFF1292 0.13 +0.0 15,502 + GFF1292 0.18 +6.1 15,601 + GFF1292 0.22 -0.6 15,605 + GFF1292 0.22 +0.0 15,606 - GFF1292 0.22 -0.3 15,608 - GFF1292 0.22 -0.6 15,643 + GFF1292 0.23 -0.6 15,854 + GFF1292 0.30 -0.3 15,858 - GFF1292 0.30 +0.1 15,914 + GFF1292 0.32 +0.2 15,914 + GFF1292 0.32 -1.1 16,299 + GFF1292 0.44 +0.1 16,300 - GFF1292 0.44 +9.5 16,733 - GFF1292 0.58 -0.7 17,199 - GFF1292 0.73 +0.9 17,327 + GFF1292 0.77 -0.7 17,642 + GFF1292 0.88 -0.1 17,643 - GFF1292 0.88 -0.5 17,714 + GFF1292 0.90 -0.1 17,732 - -0.3 17,799 + +0.2 17,800 - +0.9 17,803 - -0.9 17,827 + -0.3 17,883 - -0.6 17,947 - +0.7 17,949 - -0.8 17,950 + -0.2 17,950 + -0.8 17,951 - +0.7 18,160 + GFF1293 0.39 +1.1 18,161 - GFF1293 0.39 -1.0 18,183 + GFF1293 0.46 -1.1 18,261 + GFF1293 0.70 +0.3 18,261 + GFF1293 0.70 -0.5 18,261 + GFF1293 0.70 -0.5 18,391 - +1.1 18,536 - GFF1294 0.24 -0.3 18,689 + GFF1294 0.46 -0.2 18,693 - GFF1294 0.46 -0.4 18,693 - GFF1294 0.46 -1.0 18,693 - GFF1294 0.46 +0.1 18,694 + GFF1294 0.46 +0.3 18,694 + GFF1294 0.46 +1.7 18,694 + GFF1294 0.46 +0.3 18,695 - GFF1294 0.46 +3.2 18,695 - GFF1294 0.46 +2.1 18,695 - GFF1294 0.46 +0.2 18,807 - GFF1294 0.63 +1.1 18,888 + GFF1294 0.74 -0.4 18,896 + GFF1294 0.75 +0.4 18,919 + GFF1294 0.79 +0.5 18,921 + GFF1294 0.79 +0.4 18,957 + GFF1294 0.84 +0.2
Or see this region's nucleotide sequence