Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1198 and GFF1199 are separated by 93 nucleotides GFF1199 and GFF1200 are separated by 53 nucleotides GFF1200 and GFF1201 are separated by 89 nucleotides GFF1201 and GFF1202 are separated by 50 nucleotides GFF1202 and GFF1203 overlap by 11 nucleotides GFF1203 and GFF1204 are separated by 43 nucleotides GFF1204 and GFF1205 overlap by 35 nucleotides
GFF1198 - Porin thermoregulatory protein envY, at 44,190 to 44,948
GFF1198
GFF1199 - hypothetical protein, at 45,042 to 45,161
GFF1199
GFF1200 - Attachment invasion locus protein precursor, at 45,215 to 45,772
GFF1200
GFF1201 - Outer membrane protein assembly factor YaeT precursor, at 45,862 to 46,707
GFF1201
GFF1202 - hypothetical protein, at 46,758 to 46,904
GFF1202
GFF1203 - Thiol:disulfide interchange protein dsbA precursor, at 46,894 to 47,547
GFF1203
GFF1204 - hypothetical protein, at 47,591 to 47,704
GFF1204
GFF1205 - hypothetical protein, at 47,670 to 47,813
GFF1205
Position (kb)
45
46
47 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 44.932 kb on + strand at 44.950 kb on - strand at 44.952 kb on + strand at 44.989 kb on + strand at 44.989 kb on + strand at 44.990 kb on - strand at 45.049 kb on + strand at 45.049 kb on + strand at 45.050 kb on - strand at 45.073 kb on + strand, within GFF1199 at 45.074 kb on - strand, within GFF1199 at 45.074 kb on - strand, within GFF1199 at 45.148 kb on - strand, within GFF1199 at 45.148 kb on - strand, within GFF1199 at 45.161 kb on + strand at 45.271 kb on + strand, within GFF1200 at 45.272 kb on - strand, within GFF1200 at 45.282 kb on + strand, within GFF1200 at 45.316 kb on + strand, within GFF1200 at 45.323 kb on + strand, within GFF1200 at 45.382 kb on - strand, within GFF1200 at 45.444 kb on + strand, within GFF1200 at 45.445 kb on - strand, within GFF1200 at 45.452 kb on + strand, within GFF1200 at 45.453 kb on - strand, within GFF1200 at 45.621 kb on + strand, within GFF1200 at 45.622 kb on - strand, within GFF1200 at 45.649 kb on + strand, within GFF1200 at 45.650 kb on - strand, within GFF1200 at 45.795 kb on + strand at 45.795 kb on + strand at 45.795 kb on + strand at 46.060 kb on + strand, within GFF1201 at 46.060 kb on + strand, within GFF1201 at 46.060 kb on + strand, within GFF1201 at 46.061 kb on - strand, within GFF1201 at 46.064 kb on + strand, within GFF1201 at 46.064 kb on + strand, within GFF1201 at 46.064 kb on + strand, within GFF1201 at 46.064 kb on + strand, within GFF1201 at 46.065 kb on - strand, within GFF1201 at 46.065 kb on - strand, within GFF1201 at 46.065 kb on - strand, within GFF1201 at 46.065 kb on - strand, within GFF1201 at 46.104 kb on + strand, within GFF1201 at 46.305 kb on - strand, within GFF1201 at 46.305 kb on - strand, within GFF1201 at 46.305 kb on - strand, within GFF1201 at 46.442 kb on - strand, within GFF1201 at 46.442 kb on - strand, within GFF1201 at 46.689 kb on + strand at 46.693 kb on + strand at 46.693 kb on + strand at 46.694 kb on - strand at 46.694 kb on - strand at 46.694 kb on - strand at 46.775 kb on + strand, within GFF1202 at 46.878 kb on + strand, within GFF1202 at 47.001 kb on - strand, within GFF1203 at 47.015 kb on + strand, within GFF1203 at 47.016 kb on - strand, within GFF1203 at 47.016 kb on - strand, within GFF1203 at 47.043 kb on - strand, within GFF1203 at 47.043 kb on - strand, within GFF1203 at 47.065 kb on + strand, within GFF1203 at 47.065 kb on + strand, within GFF1203 at 47.099 kb on - strand, within GFF1203 at 47.099 kb on - strand, within GFF1203 at 47.099 kb on - strand, within GFF1203 at 47.100 kb on + strand, within GFF1203 at 47.101 kb on - strand, within GFF1203 at 47.142 kb on + strand, within GFF1203 at 47.300 kb on + strand, within GFF1203 at 47.301 kb on - strand, within GFF1203 at 47.301 kb on - strand, within GFF1203 at 47.330 kb on - strand, within GFF1203 at 47.574 kb on - strand at 47.574 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 44,932 + +0.2 44,950 - +0.3 44,952 + +0.6 44,989 + -0.6 44,989 + -0.3 44,990 - -0.2 45,049 + +1.8 45,049 + +1.2 45,050 - +1.3 45,073 + GFF1199 0.26 -0.8 45,074 - GFF1199 0.27 -0.3 45,074 - GFF1199 0.27 -0.9 45,148 - GFF1199 0.88 +0.4 45,148 - GFF1199 0.88 -0.0 45,161 + +0.8 45,271 + GFF1200 0.10 +1.6 45,272 - GFF1200 0.10 +0.4 45,282 + GFF1200 0.12 -0.2 45,316 + GFF1200 0.18 -0.5 45,323 + GFF1200 0.19 -1.4 45,382 - GFF1200 0.30 +0.1 45,444 + GFF1200 0.41 +0.1 45,445 - GFF1200 0.41 -0.4 45,452 + GFF1200 0.42 +0.3 45,453 - GFF1200 0.43 +0.3 45,621 + GFF1200 0.73 +0.5 45,622 - GFF1200 0.73 +0.2 45,649 + GFF1200 0.78 -0.1 45,650 - GFF1200 0.78 -0.6 45,795 + -0.0 45,795 + -0.1 45,795 + -0.6 46,060 + GFF1201 0.23 +1.3 46,060 + GFF1201 0.23 -0.5 46,060 + GFF1201 0.23 +0.7 46,061 - GFF1201 0.24 -0.3 46,064 + GFF1201 0.24 +0.1 46,064 + GFF1201 0.24 +2.2 46,064 + GFF1201 0.24 +1.5 46,064 + GFF1201 0.24 +0.8 46,065 - GFF1201 0.24 -0.4 46,065 - GFF1201 0.24 +1.8 46,065 - GFF1201 0.24 +2.8 46,065 - GFF1201 0.24 +0.6 46,104 + GFF1201 0.29 -1.2 46,305 - GFF1201 0.52 +0.4 46,305 - GFF1201 0.52 -0.0 46,305 - GFF1201 0.52 -0.5 46,442 - GFF1201 0.69 -0.1 46,442 - GFF1201 0.69 +0.4 46,689 + -0.6 46,693 + -0.7 46,693 + +0.2 46,694 - -0.1 46,694 - +0.6 46,694 - +1.8 46,775 + GFF1202 0.12 -0.9 46,878 + GFF1202 0.82 -0.2 47,001 - GFF1203 0.16 -1.8 47,015 + GFF1203 0.19 -0.0 47,016 - GFF1203 0.19 +2.2 47,016 - GFF1203 0.19 -0.2 47,043 - GFF1203 0.23 -0.1 47,043 - GFF1203 0.23 +1.5 47,065 + GFF1203 0.26 -0.0 47,065 + GFF1203 0.26 +1.0 47,099 - GFF1203 0.31 +1.8 47,099 - GFF1203 0.31 -0.5 47,099 - GFF1203 0.31 -0.1 47,100 + GFF1203 0.31 -0.4 47,101 - GFF1203 0.32 -0.0 47,142 + GFF1203 0.38 -1.0 47,300 + GFF1203 0.62 -0.9 47,301 - GFF1203 0.62 +0.1 47,301 - GFF1203 0.62 +0.2 47,330 - GFF1203 0.67 +1.3 47,574 - +0.6 47,574 - +0.1
Or see this region's nucleotide sequence