Experiment: LB_plus_SM_buffer with Br60_phage 0.0005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1028 and GFF1029 are separated by 120 nucleotides GFF1029 and GFF1030 are separated by 388 nucleotides GFF1030 and GFF1138 are separated by 31 nucleotides GFF1138 and GFF1139 are separated by 31 nucleotides GFF1139 and GFF1140 are separated by 27 nucleotides
GFF1028 - Ferric reductase (1.6.99.14), at 211,489 to 212,277
GFF1028
GFF1029 - Putative membrane protein, at 212,398 to 213,324
GFF1029
GFF1030 - FIG00638078: hypothetical protein, at 213,713 to 213,949
GFF1030
GFF1138 - tRNA-Leu-CAG, at 213,981 to 214,067
GFF1138
GFF1139 - tRNA-Leu-CAG, at 214,099 to 214,185
GFF1139
GFF1140 - tRNA-Leu-CAG, at 214,213 to 214,299
GFF1140
Position (kb) 212
213
214 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 211.425 kb on + strand at 211.426 kb on - strand at 211.428 kb on - strand at 211.431 kb on - strand at 211.496 kb on - strand at 211.520 kb on - strand at 211.580 kb on - strand, within GFF1028 at 211.709 kb on - strand, within GFF1028 at 211.712 kb on + strand, within GFF1028 at 211.905 kb on + strand, within GFF1028 at 211.905 kb on + strand, within GFF1028 at 211.945 kb on + strand, within GFF1028 at 211.946 kb on - strand, within GFF1028 at 211.983 kb on - strand, within GFF1028 at 211.983 kb on - strand, within GFF1028 at 211.983 kb on - strand, within GFF1028 at 212.045 kb on + strand, within GFF1028 at 212.046 kb on - strand, within GFF1028 at 212.245 kb on - strand at 212.296 kb on + strand at 212.356 kb on - strand at 212.566 kb on - strand, within GFF1029 at 212.586 kb on - strand, within GFF1029 at 212.586 kb on - strand, within GFF1029 at 212.588 kb on - strand, within GFF1029 at 212.751 kb on + strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.771 kb on + strand, within GFF1029 at 212.807 kb on + strand, within GFF1029 at 212.807 kb on + strand, within GFF1029 at 212.808 kb on - strand, within GFF1029 at 212.834 kb on - strand, within GFF1029 at 212.838 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.871 kb on - strand, within GFF1029 at 212.871 kb on - strand, within GFF1029 at 212.896 kb on - strand, within GFF1029 at 212.963 kb on + strand, within GFF1029 at 213.086 kb on + strand, within GFF1029 at 213.145 kb on - strand, within GFF1029 at 213.255 kb on + strand at 213.287 kb on - strand at 213.432 kb on + strand at 213.600 kb on - strand at 213.748 kb on - strand, within GFF1030 at 213.750 kb on + strand, within GFF1030 at 213.896 kb on - strand, within GFF1030 at 213.973 kb on - strand at 213.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.0005 MOI remove 211,425 + -0.9 211,426 - -1.4 211,428 - +1.6 211,431 - -1.1 211,496 - +0.1 211,520 - +0.4 211,580 - GFF1028 0.12 +0.6 211,709 - GFF1028 0.28 -0.0 211,712 + GFF1028 0.28 -0.6 211,905 + GFF1028 0.53 +2.1 211,905 + GFF1028 0.53 +0.3 211,945 + GFF1028 0.58 -0.5 211,946 - GFF1028 0.58 -0.0 211,983 - GFF1028 0.63 -0.6 211,983 - GFF1028 0.63 +1.3 211,983 - GFF1028 0.63 +1.6 212,045 + GFF1028 0.70 +0.3 212,046 - GFF1028 0.71 -0.6 212,245 - +0.0 212,296 + -0.1 212,356 - +0.3 212,566 - GFF1029 0.18 +3.6 212,586 - GFF1029 0.20 +0.2 212,586 - GFF1029 0.20 +1.1 212,588 - GFF1029 0.20 -2.3 212,751 + GFF1029 0.38 +0.4 212,752 - GFF1029 0.38 -1.9 212,752 - GFF1029 0.38 +1.8 212,752 - GFF1029 0.38 +0.6 212,752 - GFF1029 0.38 -0.2 212,752 - GFF1029 0.38 -0.3 212,771 + GFF1029 0.40 +1.8 212,807 + GFF1029 0.44 +0.1 212,807 + GFF1029 0.44 +0.4 212,808 - GFF1029 0.44 -0.3 212,834 - GFF1029 0.47 -0.2 212,838 + GFF1029 0.47 -1.2 212,870 + GFF1029 0.51 -0.1 212,870 + GFF1029 0.51 +0.9 212,870 + GFF1029 0.51 +0.3 212,870 + GFF1029 0.51 +0.2 212,871 - GFF1029 0.51 +0.5 212,871 - GFF1029 0.51 +0.1 212,896 - GFF1029 0.54 -1.0 212,963 + GFF1029 0.61 +0.5 213,086 + GFF1029 0.74 -0.5 213,145 - GFF1029 0.81 +0.2 213,255 + -0.4 213,287 - -0.3 213,432 + -0.7 213,600 - +4.9 213,748 - GFF1030 0.15 -0.4 213,750 + GFF1030 0.16 -0.0 213,896 - GFF1030 0.77 +0.2 213,973 - +0.3 213,978 - +0.4
Or see this region's nucleotide sequence