Experiment: R2A with Sisomicin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5178 and Atu5179 are separated by 297 nucleotides Atu5179 and Atu5181 are separated by 637 nucleotides
Atu5178: Atu5178 - ABC transporter nucleotide binding/ATPase, at 175,028 to 176,974
Atu5178
Atu5179: Atu5179 - hypothetical protein, at 177,272 to 177,562
Atu5179
Atu5181: Atu5181 - short chain dehydrogenase, at 178,200 to 178,937
Atu5181
Position (kb) 177
178 Strain fitness (log2 ratio) -1
0
1
2 at 176.274 kb on + strand, within Atu5178 at 176.275 kb on + strand, within Atu5178 at 176.282 kb on - strand, within Atu5178 at 176.350 kb on - strand, within Atu5178 at 176.422 kb on - strand, within Atu5178 at 176.452 kb on + strand, within Atu5178 at 176.555 kb on + strand, within Atu5178 at 176.562 kb on + strand, within Atu5178 at 176.567 kb on + strand, within Atu5178 at 176.575 kb on - strand, within Atu5178 at 176.594 kb on - strand, within Atu5178 at 176.655 kb on - strand, within Atu5178 at 176.732 kb on - strand, within Atu5178 at 176.740 kb on + strand, within Atu5178 at 176.754 kb on + strand, within Atu5178 at 176.756 kb on + strand, within Atu5178 at 176.761 kb on + strand, within Atu5178 at 176.883 kb on - strand at 176.886 kb on + strand at 176.894 kb on - strand at 176.947 kb on + strand at 176.957 kb on + strand at 177.011 kb on + strand at 177.072 kb on - strand at 177.125 kb on - strand at 177.181 kb on + strand at 177.245 kb on - strand at 177.249 kb on + strand at 177.274 kb on + strand at 177.314 kb on + strand, within Atu5179 at 177.317 kb on - strand, within Atu5179 at 177.332 kb on - strand, within Atu5179 at 177.383 kb on + strand, within Atu5179 at 177.391 kb on - strand, within Atu5179 at 177.402 kb on - strand, within Atu5179 at 177.445 kb on + strand, within Atu5179 at 177.445 kb on + strand, within Atu5179 at 177.453 kb on - strand, within Atu5179 at 177.549 kb on - strand at 177.584 kb on - strand at 177.670 kb on + strand at 177.673 kb on - strand at 177.793 kb on + strand at 177.798 kb on + strand at 177.806 kb on - strand at 177.857 kb on - strand at 177.904 kb on - strand at 177.932 kb on + strand at 177.948 kb on + strand at 177.956 kb on - strand at 177.956 kb on - strand at 178.060 kb on + strand at 178.075 kb on + strand at 178.196 kb on + strand at 178.198 kb on + strand at 178.204 kb on - strand at 178.204 kb on - strand at 178.204 kb on - strand at 178.204 kb on - strand at 178.204 kb on - strand at 178.206 kb on - strand at 178.229 kb on + strand at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.319 kb on + strand, within Atu5181 at 178.327 kb on - strand, within Atu5181 at 178.327 kb on - strand, within Atu5181 at 178.327 kb on - strand, within Atu5181 at 178.327 kb on - strand, within Atu5181 at 178.327 kb on - strand, within Atu5181 at 178.327 kb on - strand, within Atu5181 at 178.405 kb on + strand, within Atu5181 at 178.418 kb on + strand, within Atu5181 at 178.550 kb on + strand, within Atu5181
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.001 mM remove 176,274 + Atu5178 0.64 -0.3 176,275 + Atu5178 0.64 +0.7 176,282 - Atu5178 0.64 +2.0 176,350 - Atu5178 0.68 +0.2 176,422 - Atu5178 0.72 +0.0 176,452 + Atu5178 0.73 +0.7 176,555 + Atu5178 0.78 +1.1 176,562 + Atu5178 0.79 +0.6 176,567 + Atu5178 0.79 +0.7 176,575 - Atu5178 0.79 +0.0 176,594 - Atu5178 0.80 +1.7 176,655 - Atu5178 0.84 +0.6 176,732 - Atu5178 0.88 +0.1 176,740 + Atu5178 0.88 +0.4 176,754 + Atu5178 0.89 -1.2 176,756 + Atu5178 0.89 +0.0 176,761 + Atu5178 0.89 +0.1 176,883 - +0.1 176,886 + -0.1 176,894 - +0.0 176,947 + -0.3 176,957 + +0.0 177,011 + +0.6 177,072 - -0.2 177,125 - +1.6 177,181 + -0.6 177,245 - -0.4 177,249 + -0.6 177,274 + -0.3 177,314 + Atu5179 0.14 -0.2 177,317 - Atu5179 0.15 -0.1 177,332 - Atu5179 0.21 -0.1 177,383 + Atu5179 0.38 -0.8 177,391 - Atu5179 0.41 +0.8 177,402 - Atu5179 0.45 -0.7 177,445 + Atu5179 0.59 +0.3 177,445 + Atu5179 0.59 +0.2 177,453 - Atu5179 0.62 +0.7 177,549 - +0.4 177,584 - +1.0 177,670 + +1.3 177,673 - -0.5 177,793 + +0.3 177,798 + -0.1 177,806 - +0.6 177,857 - -0.6 177,904 - +2.2 177,932 + -0.1 177,948 + +0.0 177,956 - -0.0 177,956 - +0.5 178,060 + +0.2 178,075 + +1.1 178,196 + -0.3 178,198 + -0.5 178,204 - +0.1 178,204 - +0.6 178,204 - +0.3 178,204 - +0.2 178,204 - +0.7 178,206 - -0.6 178,229 + -1.4 178,319 + Atu5181 0.16 -0.4 178,319 + Atu5181 0.16 -0.9 178,319 + Atu5181 0.16 +0.6 178,319 + Atu5181 0.16 +0.4 178,319 + Atu5181 0.16 -0.6 178,319 + Atu5181 0.16 -1.6 178,319 + Atu5181 0.16 -0.5 178,319 + Atu5181 0.16 -0.8 178,327 - Atu5181 0.17 +0.4 178,327 - Atu5181 0.17 +0.6 178,327 - Atu5181 0.17 +1.0 178,327 - Atu5181 0.17 +0.0 178,327 - Atu5181 0.17 -0.4 178,327 - Atu5181 0.17 -0.1 178,405 + Atu5181 0.28 +0.6 178,418 + Atu5181 0.30 -0.1 178,550 + Atu5181 0.47 -0.2
Or see this region's nucleotide sequence