Experiment: Choline chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28115 and PS417_28120 are separated by 11 nucleotides PS417_28120 and PS417_28125 are separated by 333 nucleotides PS417_28125 and PS417_28130 are separated by 182 nucleotides
PS417_28115: PS417_28115 - phosphoribosylaminoimidazole carboxylase, at 6,066,820 to 6,067,905
_28115
PS417_28120: PS417_28120 - N5-carboxyaminoimidazole ribonucleotide mutase, at 6,067,917 to 6,068,408
_28120
PS417_28125: PS417_28125 - LysR family transcriptional regulator, at 6,068,742 to 6,069,650
_28125
PS417_28130: PS417_28130 - aspartate ammonia-lyase, at 6,069,833 to 6,071,257
_28130
Position (kb)
6068
6069
6070 Strain fitness (log2 ratio)
-1
0
1 at 6068.497 kb on - strand at 6068.530 kb on - strand at 6068.537 kb on - strand at 6068.539 kb on + strand at 6068.539 kb on + strand at 6068.539 kb on + strand at 6068.540 kb on - strand at 6068.548 kb on + strand at 6068.549 kb on - strand at 6068.599 kb on - strand at 6068.645 kb on - strand at 6068.693 kb on - strand at 6070.226 kb on + strand, within PS417_28130
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 300 mM remove 6,068,497 - +0.2 6,068,530 - +0.6 6,068,537 - -1.1 6,068,539 + +0.3 6,068,539 + +0.4 6,068,539 + -0.1 6,068,540 - -0.3 6,068,548 + +0.2 6,068,549 - +0.1 6,068,599 - +0.8 6,068,645 - +1.1 6,068,693 - +0.7 6,070,226 + PS417_28130 0.28 +0.1
Or see this region's nucleotide sequence