Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4822 and Atu4823 overlap by 4 nucleotides Atu4823 and Atu4824 overlap by 4 nucleotides Atu4824 and Atu4825 are separated by 1 nucleotides
Atu4822: Atu4822 - acyl-CoA synthetase, at 2,001,585 to 2,003,225
Atu4822
Atu4823: Atu4823 - acetyl-CoA acetyltransferase, at 2,003,222 to 2,004,391
Atu4823
Atu4824: Atu4824 - IclR family transcriptional regulator, at 2,004,388 to 2,005,254
Atu4824
Atu4825: Atu4825 - dehydrogenase, at 2,005,256 to 2,006,071
Atu4825
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-2
-1
0
1 at 2002.224 kb on - strand, within Atu4822 at 2002.297 kb on + strand, within Atu4822 at 2002.318 kb on + strand, within Atu4822 at 2002.367 kb on + strand, within Atu4822 at 2002.400 kb on - strand, within Atu4822 at 2002.402 kb on + strand, within Atu4822 at 2002.509 kb on - strand, within Atu4822 at 2002.520 kb on + strand, within Atu4822 at 2002.637 kb on - strand, within Atu4822 at 2002.661 kb on + strand, within Atu4822 at 2002.701 kb on - strand, within Atu4822 at 2002.750 kb on + strand, within Atu4822 at 2002.764 kb on + strand, within Atu4822 at 2002.772 kb on - strand, within Atu4822 at 2002.846 kb on + strand, within Atu4822 at 2002.851 kb on + strand, within Atu4822 at 2002.912 kb on - strand, within Atu4822 at 2002.929 kb on + strand, within Atu4822 at 2003.087 kb on - strand at 2003.114 kb on + strand at 2003.176 kb on - strand at 2003.242 kb on - strand at 2003.445 kb on + strand, within Atu4823 at 2003.450 kb on + strand, within Atu4823 at 2003.458 kb on - strand, within Atu4823 at 2003.636 kb on + strand, within Atu4823 at 2003.636 kb on + strand, within Atu4823 at 2003.644 kb on - strand, within Atu4823 at 2003.644 kb on - strand, within Atu4823 at 2003.768 kb on + strand, within Atu4823 at 2003.771 kb on - strand, within Atu4823 at 2004.008 kb on + strand, within Atu4823 at 2004.013 kb on + strand, within Atu4823 at 2004.033 kb on - strand, within Atu4823 at 2004.108 kb on + strand, within Atu4823 at 2004.109 kb on - strand, within Atu4823 at 2004.116 kb on - strand, within Atu4823 at 2004.116 kb on - strand, within Atu4823 at 2004.121 kb on - strand, within Atu4823 at 2004.544 kb on + strand, within Atu4824 at 2004.579 kb on - strand, within Atu4824 at 2004.628 kb on + strand, within Atu4824 at 2004.677 kb on + strand, within Atu4824 at 2004.749 kb on - strand, within Atu4824 at 2004.829 kb on - strand, within Atu4824 at 2004.849 kb on - strand, within Atu4824 at 2004.898 kb on - strand, within Atu4824 at 2004.987 kb on + strand, within Atu4824 at 2005.040 kb on + strand, within Atu4824 at 2005.048 kb on - strand, within Atu4824 at 2005.048 kb on - strand, within Atu4824 at 2005.064 kb on + strand, within Atu4824 at 2005.069 kb on + strand, within Atu4824 at 2005.072 kb on - strand, within Atu4824 at 2005.281 kb on - strand at 2005.288 kb on - strand at 2005.330 kb on + strand at 2005.378 kb on - strand, within Atu4825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 2,002,224 - Atu4822 0.39 +0.2 2,002,297 + Atu4822 0.43 +0.0 2,002,318 + Atu4822 0.45 +0.0 2,002,367 + Atu4822 0.48 +0.0 2,002,400 - Atu4822 0.50 -0.9 2,002,402 + Atu4822 0.50 -0.9 2,002,509 - Atu4822 0.56 -0.5 2,002,520 + Atu4822 0.57 -0.5 2,002,637 - Atu4822 0.64 -1.3 2,002,661 + Atu4822 0.66 +0.2 2,002,701 - Atu4822 0.68 -0.8 2,002,750 + Atu4822 0.71 +0.0 2,002,764 + Atu4822 0.72 -0.0 2,002,772 - Atu4822 0.72 -0.8 2,002,846 + Atu4822 0.77 -0.3 2,002,851 + Atu4822 0.77 -0.3 2,002,912 - Atu4822 0.81 -0.2 2,002,929 + Atu4822 0.82 -0.1 2,003,087 - +0.2 2,003,114 + -1.0 2,003,176 - -1.2 2,003,242 - -0.4 2,003,445 + Atu4823 0.19 -0.9 2,003,450 + Atu4823 0.19 -2.1 2,003,458 - Atu4823 0.20 -0.1 2,003,636 + Atu4823 0.35 -1.3 2,003,636 + Atu4823 0.35 +0.3 2,003,644 - Atu4823 0.36 -0.1 2,003,644 - Atu4823 0.36 -0.0 2,003,768 + Atu4823 0.47 -0.4 2,003,771 - Atu4823 0.47 +0.1 2,004,008 + Atu4823 0.67 -0.1 2,004,013 + Atu4823 0.68 -0.4 2,004,033 - Atu4823 0.69 -0.4 2,004,108 + Atu4823 0.76 +0.0 2,004,109 - Atu4823 0.76 -0.0 2,004,116 - Atu4823 0.76 -0.7 2,004,116 - Atu4823 0.76 -0.2 2,004,121 - Atu4823 0.77 -0.4 2,004,544 + Atu4824 0.18 +0.7 2,004,579 - Atu4824 0.22 -0.6 2,004,628 + Atu4824 0.28 -0.5 2,004,677 + Atu4824 0.33 -0.2 2,004,749 - Atu4824 0.42 +0.7 2,004,829 - Atu4824 0.51 +0.2 2,004,849 - Atu4824 0.53 -0.6 2,004,898 - Atu4824 0.59 -0.2 2,004,987 + Atu4824 0.69 +0.6 2,005,040 + Atu4824 0.75 -0.2 2,005,048 - Atu4824 0.76 +0.1 2,005,048 - Atu4824 0.76 -0.4 2,005,064 + Atu4824 0.78 -0.2 2,005,069 + Atu4824 0.79 +0.2 2,005,072 - Atu4824 0.79 -0.4 2,005,281 - +0.5 2,005,288 - +0.3 2,005,330 + +1.0 2,005,378 - Atu4825 0.15 +0.7
Or see this region's nucleotide sequence