Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0971 and Atu0972 are separated by 207 nucleotides Atu0972 and Atu0973 are separated by 150 nucleotides
Atu0971: Atu0971 - two component sensor kinase, at 959,513 to 960,913
Atu0971
Atu0972: Atu0972 - lipA protein, at 961,121 to 961,549
Atu0972
Atu0973: Atu0973 - cycH protein, at 961,700 to 962,872
Atu0973
Position (kb) 961
962 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 960.144 kb on - strand, within Atu0971 at 960.242 kb on - strand, within Atu0971 at 960.380 kb on + strand, within Atu0971 at 960.551 kb on - strand, within Atu0971 at 960.581 kb on + strand, within Atu0971 at 960.618 kb on - strand, within Atu0971 at 960.654 kb on - strand, within Atu0971 at 960.738 kb on - strand, within Atu0971 at 960.764 kb on + strand, within Atu0971 at 961.056 kb on + strand at 961.089 kb on - strand at 961.248 kb on - strand, within Atu0972 at 961.326 kb on - strand, within Atu0972 at 961.358 kb on + strand, within Atu0972 at 961.373 kb on + strand, within Atu0972 at 961.396 kb on + strand, within Atu0972 at 961.410 kb on - strand, within Atu0972 at 961.488 kb on + strand, within Atu0972 at 961.538 kb on + strand at 961.546 kb on - strand at 961.589 kb on + strand at 961.603 kb on + strand at 961.611 kb on - strand at 961.611 kb on - strand at 961.642 kb on - strand at 961.684 kb on - strand at 961.712 kb on - strand at 962.211 kb on + strand, within Atu0973 at 962.213 kb on - strand, within Atu0973 at 962.222 kb on - strand, within Atu0973 at 962.438 kb on + strand, within Atu0973
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 960,144 - Atu0971 0.45 -4.2 960,242 - Atu0971 0.52 -0.8 960,380 + Atu0971 0.62 -2.5 960,551 - Atu0971 0.74 -2.6 960,581 + Atu0971 0.76 -3.4 960,618 - Atu0971 0.79 -0.1 960,654 - Atu0971 0.81 -1.4 960,738 - Atu0971 0.87 -1.6 960,764 + Atu0971 0.89 -1.3 961,056 + -0.3 961,089 - -0.8 961,248 - Atu0972 0.30 -1.2 961,326 - Atu0972 0.48 -2.2 961,358 + Atu0972 0.55 -1.3 961,373 + Atu0972 0.59 -0.1 961,396 + Atu0972 0.64 -1.2 961,410 - Atu0972 0.67 -0.9 961,488 + Atu0972 0.86 -1.3 961,538 + -2.0 961,546 - -0.0 961,589 + -0.1 961,603 + -0.3 961,611 - +0.8 961,611 - -0.8 961,642 - +0.1 961,684 - -1.3 961,712 - +0.4 962,211 + Atu0973 0.44 +0.3 962,213 - Atu0973 0.44 -1.6 962,222 - Atu0973 0.45 -0.7 962,438 + Atu0973 0.63 -0.1
Or see this region's nucleotide sequence