Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0597 and Atu0598 are separated by 218 nucleotides Atu0598 and Atu0599 are separated by 124 nucleotides Atu0599 and Atu0600 are separated by 10 nucleotides
Atu0597: Atu0597 - hypothetical protein, at 581,615 to 582,976
Atu0597
Atu0598: Atu0598 - phosphogluconate dehydratase, at 583,195 to 585,015
Atu0598
Atu0599: Atu0599 - 6-phosphogluconolactonase, at 585,140 to 585,838
Atu0599
Atu0600: Atu0600 - glucose-6-phosphate 1-dehydrogenase, at 585,849 to 587,324
Atu0600
Position (kb) 583
584
585
586 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 582.202 kb on + strand, within Atu0597 at 582.239 kb on - strand, within Atu0597 at 582.261 kb on + strand, within Atu0597 at 582.297 kb on + strand, within Atu0597 at 582.352 kb on + strand, within Atu0597 at 582.355 kb on - strand, within Atu0597 at 582.396 kb on + strand, within Atu0597 at 582.526 kb on - strand, within Atu0597 at 582.669 kb on + strand, within Atu0597 at 582.828 kb on - strand, within Atu0597 at 582.860 kb on - strand at 582.889 kb on + strand at 582.949 kb on - strand at 582.991 kb on + strand at 582.991 kb on + strand at 582.999 kb on - strand at 583.045 kb on + strand at 583.160 kb on + strand at 583.551 kb on - strand, within Atu0598 at 583.620 kb on + strand, within Atu0598 at 583.809 kb on + strand, within Atu0598 at 583.817 kb on - strand, within Atu0598 at 583.817 kb on - strand, within Atu0598 at 584.104 kb on + strand, within Atu0598 at 584.305 kb on - strand, within Atu0598 at 584.477 kb on + strand, within Atu0598 at 584.561 kb on - strand, within Atu0598 at 584.592 kb on + strand, within Atu0598 at 584.655 kb on + strand, within Atu0598 at 584.661 kb on + strand, within Atu0598 at 584.887 kb on + strand at 584.895 kb on - strand at 584.957 kb on + strand at 584.992 kb on - strand at 585.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 582,202 + Atu0597 0.43 +0.3 582,239 - Atu0597 0.46 -0.4 582,261 + Atu0597 0.47 -1.0 582,297 + Atu0597 0.50 -0.1 582,352 + Atu0597 0.54 -0.5 582,355 - Atu0597 0.54 +0.3 582,396 + Atu0597 0.57 +0.0 582,526 - Atu0597 0.67 +0.5 582,669 + Atu0597 0.77 -0.2 582,828 - Atu0597 0.89 -0.1 582,860 - -0.1 582,889 + +0.1 582,949 - -0.8 582,991 + -0.3 582,991 + +1.3 582,999 - +0.2 583,045 + -0.3 583,160 + -0.2 583,551 - Atu0598 0.20 -0.6 583,620 + Atu0598 0.23 -2.3 583,809 + Atu0598 0.34 -2.7 583,817 - Atu0598 0.34 -3.7 583,817 - Atu0598 0.34 -3.2 584,104 + Atu0598 0.50 -3.3 584,305 - Atu0598 0.61 -2.3 584,477 + Atu0598 0.70 -2.9 584,561 - Atu0598 0.75 -3.8 584,592 + Atu0598 0.77 -3.7 584,655 + Atu0598 0.80 -3.5 584,661 + Atu0598 0.81 -4.2 584,887 + -1.2 584,895 - -3.1 584,957 + -3.4 584,992 - -1.5 585,123 - -0.1
Or see this region's nucleotide sequence