Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0293 and Atu0294 are separated by 159 nucleotides Atu0294 and Atu0295 are separated by 239 nucleotides Atu0295 and Atu0296 are separated by 88 nucleotides
Atu0293: Atu0293 - glutathione S-transferase, at 287,938 to 288,630
Atu0293
Atu0294: Atu0294 - undecaprenyl pyrophosphate phosphatase, possible bacitracin resistance protein, at 288,790 to 289,596
Atu0294
Atu0295: Atu0295 - hypothetical protein, at 289,836 to 290,066
Atu0295
Atu0296: Atu0296 - NADH-ubiquinone oxidoreductase, at 290,155 to 291,135
Atu0296
Position (kb) 288
289
290 Strain fitness (log2 ratio) -1
0
1 at 287.834 kb on + strand at 287.842 kb on - strand at 288.786 kb on + strand at 288.794 kb on - strand at 288.924 kb on - strand, within Atu0294 at 289.016 kb on + strand, within Atu0294 at 289.016 kb on + strand, within Atu0294 at 289.016 kb on + strand, within Atu0294 at 289.024 kb on - strand, within Atu0294 at 289.024 kb on - strand, within Atu0294 at 289.031 kb on + strand, within Atu0294 at 289.039 kb on - strand, within Atu0294 at 289.087 kb on + strand, within Atu0294 at 289.108 kb on + strand, within Atu0294 at 289.149 kb on - strand, within Atu0294 at 289.255 kb on - strand, within Atu0294 at 289.314 kb on + strand, within Atu0294 at 289.320 kb on - strand, within Atu0294 at 289.455 kb on - strand, within Atu0294 at 289.585 kb on - strand at 289.603 kb on + strand at 289.689 kb on + strand at 289.697 kb on - strand at 289.709 kb on + strand at 289.788 kb on + strand at 289.870 kb on + strand, within Atu0295 at 289.951 kb on - strand, within Atu0295 at 289.963 kb on + strand, within Atu0295 at 290.019 kb on - strand, within Atu0295 at 290.154 kb on - strand at 290.154 kb on - strand at 290.215 kb on - strand at 290.371 kb on - strand, within Atu0296 at 290.473 kb on - strand, within Atu0296 at 290.485 kb on - strand, within Atu0296
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 287,834 + +1.2 287,842 - +0.2 288,786 + +0.1 288,794 - -0.6 288,924 - Atu0294 0.17 -0.8 289,016 + Atu0294 0.28 -0.1 289,016 + Atu0294 0.28 +0.4 289,016 + Atu0294 0.28 -0.4 289,024 - Atu0294 0.29 +0.4 289,024 - Atu0294 0.29 +0.5 289,031 + Atu0294 0.30 +0.2 289,039 - Atu0294 0.31 -0.9 289,087 + Atu0294 0.37 +0.0 289,108 + Atu0294 0.39 +0.1 289,149 - Atu0294 0.44 -0.2 289,255 - Atu0294 0.58 -0.7 289,314 + Atu0294 0.65 +0.3 289,320 - Atu0294 0.66 +0.4 289,455 - Atu0294 0.82 +1.3 289,585 - -0.4 289,603 + +0.0 289,689 + -0.2 289,697 - -0.1 289,709 + +0.0 289,788 + +1.6 289,870 + Atu0295 0.15 -1.7 289,951 - Atu0295 0.50 -1.3 289,963 + Atu0295 0.55 -0.8 290,019 - Atu0295 0.79 +0.2 290,154 - +0.0 290,154 - +0.5 290,215 - +0.2 290,371 - Atu0296 0.22 +1.2 290,473 - Atu0296 0.32 +0.6 290,485 - Atu0296 0.34 +0.0
Or see this region's nucleotide sequence