Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0186 and Atu0187 are separated by 202 nucleotides Atu0187 and Atu0188 are separated by 141 nucleotides
Atu0186: Atu0186 - penicillin-insensitive murein endopeptidase, at 188,270 to 189,325
Atu0186
Atu0187: Atu0187 - ABC transporter, substrate binding protein (peptide), at 189,528 to 191,372
Atu0187
Atu0188: Atu0188 - ABC transporter, membrane spanning protein (peptide), at 191,514 to 192,608
Atu0188
Position (kb) 189
190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 188.530 kb on + strand, within Atu0186 at 188.575 kb on - strand, within Atu0186 at 188.575 kb on - strand, within Atu0186 at 188.705 kb on - strand, within Atu0186 at 188.745 kb on + strand, within Atu0186 at 188.786 kb on + strand, within Atu0186 at 188.872 kb on + strand, within Atu0186 at 188.872 kb on + strand, within Atu0186 at 188.872 kb on + strand, within Atu0186 at 188.872 kb on + strand, within Atu0186 at 188.892 kb on - strand, within Atu0186 at 188.892 kb on - strand, within Atu0186 at 188.892 kb on - strand, within Atu0186 at 188.925 kb on + strand, within Atu0186 at 189.097 kb on - strand, within Atu0186 at 189.132 kb on + strand, within Atu0186 at 189.177 kb on - strand, within Atu0186 at 189.235 kb on - strand at 189.235 kb on - strand at 189.235 kb on - strand at 189.257 kb on + strand at 189.304 kb on - strand at 189.411 kb on - strand at 189.482 kb on + strand at 189.550 kb on + strand at 189.584 kb on + strand at 189.611 kb on + strand at 189.750 kb on - strand, within Atu0187 at 189.818 kb on - strand, within Atu0187 at 189.853 kb on - strand, within Atu0187 at 189.874 kb on - strand, within Atu0187 at 189.963 kb on + strand, within Atu0187 at 190.010 kb on + strand, within Atu0187 at 190.030 kb on + strand, within Atu0187 at 190.030 kb on + strand, within Atu0187 at 190.038 kb on - strand, within Atu0187 at 190.052 kb on + strand, within Atu0187 at 190.123 kb on + strand, within Atu0187 at 190.123 kb on - strand, within Atu0187 at 190.205 kb on - strand, within Atu0187 at 190.212 kb on + strand, within Atu0187 at 190.212 kb on - strand, within Atu0187 at 190.252 kb on + strand, within Atu0187 at 190.252 kb on + strand, within Atu0187 at 190.260 kb on - strand, within Atu0187 at 190.260 kb on - strand, within Atu0187 at 190.401 kb on + strand, within Atu0187 at 190.510 kb on + strand, within Atu0187 at 190.571 kb on - strand, within Atu0187 at 190.600 kb on - strand, within Atu0187 at 190.743 kb on + strand, within Atu0187 at 190.743 kb on + strand, within Atu0187 at 190.878 kb on + strand, within Atu0187 at 190.918 kb on + strand, within Atu0187 at 190.977 kb on + strand, within Atu0187 at 190.992 kb on - strand, within Atu0187 at 191.001 kb on - strand, within Atu0187 at 191.044 kb on - strand, within Atu0187 at 191.237 kb on + strand at 191.237 kb on + strand at 191.245 kb on - strand at 191.245 kb on - strand at 191.301 kb on - strand at 191.361 kb on + strand at 191.505 kb on - strand at 191.544 kb on - strand at 191.669 kb on + strand, within Atu0188 at 191.760 kb on - strand, within Atu0188 at 191.937 kb on - strand, within Atu0188
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 188,530 + Atu0186 0.25 -0.3 188,575 - Atu0186 0.29 +0.1 188,575 - Atu0186 0.29 +0.3 188,705 - Atu0186 0.41 -0.1 188,745 + Atu0186 0.45 +0.3 188,786 + Atu0186 0.49 -0.2 188,872 + Atu0186 0.57 +0.7 188,872 + Atu0186 0.57 -0.2 188,872 + Atu0186 0.57 -0.4 188,872 + Atu0186 0.57 -0.0 188,892 - Atu0186 0.59 +0.2 188,892 - Atu0186 0.59 -0.1 188,892 - Atu0186 0.59 +0.1 188,925 + Atu0186 0.62 -0.4 189,097 - Atu0186 0.78 -0.2 189,132 + Atu0186 0.82 -0.1 189,177 - Atu0186 0.86 -0.8 189,235 - +0.2 189,235 - +0.1 189,235 - +0.3 189,257 + +0.5 189,304 - +0.2 189,411 - -0.0 189,482 + -0.5 189,550 + -0.7 189,584 + +0.1 189,611 + +0.5 189,750 - Atu0187 0.12 -0.7 189,818 - Atu0187 0.16 -0.7 189,853 - Atu0187 0.18 -0.7 189,874 - Atu0187 0.19 -1.1 189,963 + Atu0187 0.24 -2.7 190,010 + Atu0187 0.26 -1.5 190,030 + Atu0187 0.27 -1.3 190,030 + Atu0187 0.27 -1.0 190,038 - Atu0187 0.28 -1.5 190,052 + Atu0187 0.28 -0.3 190,123 + Atu0187 0.32 -0.9 190,123 - Atu0187 0.32 -1.9 190,205 - Atu0187 0.37 -0.1 190,212 + Atu0187 0.37 -0.9 190,212 - Atu0187 0.37 -0.8 190,252 + Atu0187 0.39 -1.4 190,252 + Atu0187 0.39 -0.4 190,260 - Atu0187 0.40 -0.9 190,260 - Atu0187 0.40 -1.4 190,401 + Atu0187 0.47 -1.2 190,510 + Atu0187 0.53 -0.7 190,571 - Atu0187 0.57 -1.1 190,600 - Atu0187 0.58 -1.1 190,743 + Atu0187 0.66 -1.3 190,743 + Atu0187 0.66 -0.4 190,878 + Atu0187 0.73 -1.4 190,918 + Atu0187 0.75 -0.7 190,977 + Atu0187 0.79 +0.3 190,992 - Atu0187 0.79 +1.5 191,001 - Atu0187 0.80 -0.7 191,044 - Atu0187 0.82 -1.0 191,237 + +0.1 191,237 + -1.2 191,245 - -0.8 191,245 - -2.8 191,301 - +0.1 191,361 + -0.6 191,505 - -2.3 191,544 - -2.2 191,669 + Atu0188 0.14 -2.3 191,760 - Atu0188 0.22 +0.5 191,937 - Atu0188 0.39 -2.0
Or see this region's nucleotide sequence