Experiment: R2A with Gentamicin 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0102 and Atu0103 are separated by 288 nucleotides Atu0103 and Atu0104 are separated by 116 nucleotides Atu0104 and Atu0105 are separated by 9 nucleotides
Atu0102: Atu0102 - exopolysaccharide production protein, at 105,470 to 106,195
Atu0102
Atu0103: Atu0103 - penicillin-binding protein, at 106,484 to 108,778
Atu0103
Atu0104: Atu0104 - hypothetical protein, at 108,895 to 109,353
Atu0104
Atu0105: Atu0105 - hypothetical protein, at 109,363 to 109,557
Atu0105
Position (kb)
106
107
108
109 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 105.526 kb on - strand at 105.552 kb on + strand, within Atu0102 at 105.552 kb on + strand, within Atu0102 at 105.723 kb on + strand, within Atu0102 at 105.729 kb on - strand, within Atu0102 at 105.866 kb on - strand, within Atu0102 at 105.866 kb on - strand, within Atu0102 at 105.947 kb on + strand, within Atu0102 at 105.948 kb on - strand, within Atu0102 at 106.003 kb on - strand, within Atu0102 at 106.003 kb on - strand, within Atu0102 at 106.003 kb on - strand, within Atu0102 at 106.034 kb on - strand, within Atu0102 at 106.041 kb on - strand, within Atu0102 at 106.043 kb on + strand, within Atu0102 at 106.043 kb on + strand, within Atu0102 at 106.179 kb on - strand at 106.192 kb on + strand at 106.333 kb on + strand at 106.444 kb on - strand at 106.730 kb on + strand, within Atu0103 at 106.785 kb on - strand, within Atu0103 at 106.808 kb on + strand, within Atu0103 at 106.973 kb on + strand, within Atu0103 at 107.003 kb on + strand, within Atu0103 at 107.033 kb on - strand, within Atu0103 at 107.044 kb on - strand, within Atu0103 at 107.050 kb on + strand, within Atu0103 at 107.236 kb on + strand, within Atu0103 at 107.236 kb on + strand, within Atu0103 at 107.236 kb on + strand, within Atu0103 at 107.244 kb on - strand, within Atu0103 at 107.316 kb on - strand, within Atu0103 at 107.425 kb on - strand, within Atu0103 at 107.483 kb on + strand, within Atu0103 at 107.706 kb on + strand, within Atu0103 at 107.706 kb on + strand, within Atu0103 at 107.811 kb on + strand, within Atu0103 at 107.954 kb on - strand, within Atu0103 at 108.009 kb on - strand, within Atu0103 at 108.101 kb on + strand, within Atu0103 at 108.101 kb on + strand, within Atu0103 at 108.101 kb on + strand, within Atu0103 at 108.227 kb on + strand, within Atu0103 at 108.227 kb on + strand, within Atu0103 at 108.227 kb on + strand, within Atu0103 at 108.235 kb on - strand, within Atu0103 at 108.235 kb on - strand, within Atu0103 at 108.235 kb on - strand, within Atu0103 at 108.235 kb on - strand, within Atu0103 at 108.240 kb on - strand, within Atu0103 at 108.357 kb on + strand, within Atu0103 at 108.369 kb on - strand, within Atu0103 at 108.421 kb on - strand, within Atu0103 at 108.489 kb on + strand, within Atu0103 at 108.522 kb on - strand, within Atu0103 at 108.551 kb on + strand at 108.551 kb on + strand at 108.559 kb on - strand at 108.640 kb on - strand at 108.664 kb on + strand at 108.675 kb on + strand at 108.699 kb on + strand at 108.805 kb on + strand at 108.805 kb on + strand at 108.805 kb on + strand at 108.813 kb on - strand at 108.830 kb on - strand at 108.867 kb on + strand at 108.906 kb on + strand at 108.914 kb on - strand at 108.914 kb on - strand at 108.925 kb on + strand at 109.037 kb on + strand, within Atu0104 at 109.045 kb on - strand, within Atu0104 at 109.055 kb on - strand, within Atu0104 at 109.161 kb on - strand, within Atu0104 at 109.169 kb on - strand, within Atu0104 at 109.203 kb on + strand, within Atu0104 at 109.238 kb on - strand, within Atu0104 at 109.249 kb on - strand, within Atu0104 at 109.253 kb on + strand, within Atu0104 at 109.263 kb on + strand, within Atu0104 at 109.263 kb on + strand, within Atu0104 at 109.271 kb on - strand, within Atu0104 at 109.290 kb on - strand, within Atu0104 at 109.299 kb on + strand, within Atu0104 at 109.305 kb on + strand, within Atu0104 at 109.307 kb on - strand, within Atu0104 at 109.309 kb on + strand at 109.464 kb on - strand, within Atu0105 at 109.499 kb on - strand, within Atu0105 at 109.501 kb on - strand, within Atu0105 at 109.637 kb on - strand at 109.644 kb on - strand at 109.646 kb on + strand at 109.646 kb on + strand at 109.646 kb on + strand at 109.672 kb on + strand at 109.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Gentamicin 0.00125 mM remove 105,526 - +0.1 105,552 + Atu0102 0.11 +0.7 105,552 + Atu0102 0.11 +1.1 105,723 + Atu0102 0.35 -1.1 105,729 - Atu0102 0.36 -1.5 105,866 - Atu0102 0.55 -0.1 105,866 - Atu0102 0.55 +1.5 105,947 + Atu0102 0.66 -0.7 105,948 - Atu0102 0.66 +1.1 106,003 - Atu0102 0.73 +0.3 106,003 - Atu0102 0.73 -1.4 106,003 - Atu0102 0.73 +1.2 106,034 - Atu0102 0.78 -0.2 106,041 - Atu0102 0.79 -0.1 106,043 + Atu0102 0.79 +0.7 106,043 + Atu0102 0.79 -0.3 106,179 - -0.7 106,192 + -0.3 106,333 + +0.7 106,444 - -0.2 106,730 + Atu0103 0.11 +0.2 106,785 - Atu0103 0.13 +0.2 106,808 + Atu0103 0.14 -0.0 106,973 + Atu0103 0.21 -0.9 107,003 + Atu0103 0.23 -2.8 107,033 - Atu0103 0.24 -4.0 107,044 - Atu0103 0.24 -3.5 107,050 + Atu0103 0.25 -2.8 107,236 + Atu0103 0.33 -2.2 107,236 + Atu0103 0.33 -2.9 107,236 + Atu0103 0.33 -3.5 107,244 - Atu0103 0.33 +0.1 107,316 - Atu0103 0.36 -2.6 107,425 - Atu0103 0.41 -3.8 107,483 + Atu0103 0.44 -4.2 107,706 + Atu0103 0.53 -4.7 107,706 + Atu0103 0.53 -2.8 107,811 + Atu0103 0.58 -4.3 107,954 - Atu0103 0.64 -4.0 108,009 - Atu0103 0.66 -3.6 108,101 + Atu0103 0.70 -3.1 108,101 + Atu0103 0.70 -2.6 108,101 + Atu0103 0.70 -2.6 108,227 + Atu0103 0.76 -0.7 108,227 + Atu0103 0.76 -3.8 108,227 + Atu0103 0.76 -4.0 108,235 - Atu0103 0.76 -2.5 108,235 - Atu0103 0.76 -1.9 108,235 - Atu0103 0.76 -4.0 108,235 - Atu0103 0.76 -3.6 108,240 - Atu0103 0.77 -4.3 108,357 + Atu0103 0.82 -5.8 108,369 - Atu0103 0.82 -1.9 108,421 - Atu0103 0.84 -4.2 108,489 + Atu0103 0.87 -0.3 108,522 - Atu0103 0.89 -2.4 108,551 + -2.7 108,551 + -1.0 108,559 - -2.6 108,640 - -1.5 108,664 + -2.5 108,675 + -1.7 108,699 + -1.1 108,805 + +0.2 108,805 + -0.3 108,805 + -0.5 108,813 - -0.3 108,830 - +0.3 108,867 + -0.1 108,906 + -0.3 108,914 - -0.0 108,914 - +0.0 108,925 + +0.1 109,037 + Atu0104 0.31 -1.5 109,045 - Atu0104 0.33 -1.3 109,055 - Atu0104 0.35 -2.0 109,161 - Atu0104 0.58 -0.2 109,169 - Atu0104 0.60 -0.2 109,203 + Atu0104 0.67 +0.0 109,238 - Atu0104 0.75 +0.2 109,249 - Atu0104 0.77 -0.6 109,253 + Atu0104 0.78 -1.0 109,263 + Atu0104 0.80 -0.1 109,263 + Atu0104 0.80 -0.9 109,271 - Atu0104 0.82 -0.1 109,290 - Atu0104 0.86 +0.1 109,299 + Atu0104 0.88 -0.4 109,305 + Atu0104 0.89 -0.7 109,307 - Atu0104 0.90 -0.1 109,309 + +0.3 109,464 - Atu0105 0.52 +0.1 109,499 - Atu0105 0.70 -0.3 109,501 - Atu0105 0.71 +0.1 109,637 - +0.0 109,644 - +0.7 109,646 + +0.2 109,646 + -0.2 109,646 + +0.3 109,672 + +0.6 109,680 - -0.2
Or see this region's nucleotide sequence