Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2285 and B158DRAFT_2287 are separated by 832 nucleotides B158DRAFT_2287 and B158DRAFT_2288 are separated by 23 nucleotides
B158DRAFT_2285: B158DRAFT_2285 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 450,437 to 451,138
_2285
B158DRAFT_2287: B158DRAFT_2287 - ATP phosphoribosyltransferase, at 451,971 to 452,819
_2287
B158DRAFT_2288: B158DRAFT_2288 - haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E, at 452,843 to 454,540
_2288
Position (kb)
451
452
453 Strain fitness (log2 ratio)
-1
0
1
2 at 451.156 kb on - strand at 451.158 kb on + strand at 451.166 kb on - strand at 451.166 kb on - strand at 451.166 kb on - strand at 451.178 kb on - strand at 451.318 kb on - strand at 451.318 kb on - strand at 451.346 kb on - strand at 451.409 kb on + strand at 451.431 kb on + strand at 451.470 kb on + strand at 451.475 kb on + strand at 451.478 kb on - strand at 451.478 kb on - strand at 451.490 kb on + strand at 451.496 kb on + strand at 451.498 kb on - strand at 451.576 kb on + strand at 451.591 kb on + strand at 451.599 kb on - strand at 451.599 kb on - strand at 451.806 kb on + strand at 451.806 kb on + strand at 451.806 kb on + strand at 451.842 kb on - strand at 451.842 kb on - strand at 451.888 kb on + strand at 451.912 kb on - strand at 452.082 kb on + strand, within B158DRAFT_2287 at 452.118 kb on + strand, within B158DRAFT_2287 at 452.118 kb on + strand, within B158DRAFT_2287 at 452.166 kb on - strand, within B158DRAFT_2287 at 452.203 kb on + strand, within B158DRAFT_2287 at 452.211 kb on - strand, within B158DRAFT_2287 at 452.228 kb on + strand, within B158DRAFT_2287 at 452.259 kb on + strand, within B158DRAFT_2287 at 452.259 kb on + strand, within B158DRAFT_2287 at 452.259 kb on + strand, within B158DRAFT_2287 at 452.289 kb on - strand, within B158DRAFT_2287 at 452.369 kb on + strand, within B158DRAFT_2287 at 452.536 kb on - strand, within B158DRAFT_2287 at 452.583 kb on + strand, within B158DRAFT_2287 at 452.583 kb on + strand, within B158DRAFT_2287 at 452.583 kb on + strand, within B158DRAFT_2287 at 452.585 kb on + strand, within B158DRAFT_2287 at 452.591 kb on - strand, within B158DRAFT_2287 at 452.592 kb on - strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.631 kb on - strand, within B158DRAFT_2287 at 452.631 kb on - strand, within B158DRAFT_2287 at 452.646 kb on + strand, within B158DRAFT_2287 at 452.700 kb on + strand, within B158DRAFT_2287 at 452.832 kb on + strand at 452.832 kb on + strand at 452.832 kb on + strand at 452.832 kb on + strand at 452.883 kb on + strand at 452.916 kb on + strand at 452.916 kb on + strand at 452.947 kb on - strand at 452.953 kb on + strand at 452.953 kb on + strand at 452.958 kb on + strand at 452.958 kb on + strand at 452.958 kb on + strand at 452.962 kb on + strand at 452.966 kb on - strand at 453.018 kb on + strand, within B158DRAFT_2288 at 453.075 kb on + strand, within B158DRAFT_2288 at 453.075 kb on + strand, within B158DRAFT_2288 at 453.083 kb on - strand, within B158DRAFT_2288 at 453.083 kb on - strand, within B158DRAFT_2288 at 453.132 kb on + strand, within B158DRAFT_2288 at 453.140 kb on - strand, within B158DRAFT_2288 at 453.168 kb on - strand, within B158DRAFT_2288 at 453.205 kb on + strand, within B158DRAFT_2288 at 453.250 kb on + strand, within B158DRAFT_2288 at 453.262 kb on - strand, within B158DRAFT_2288 at 453.276 kb on + strand, within B158DRAFT_2288 at 453.286 kb on + strand, within B158DRAFT_2288 at 453.294 kb on - strand, within B158DRAFT_2288 at 453.294 kb on - strand, within B158DRAFT_2288 at 453.374 kb on - strand, within B158DRAFT_2288 at 453.406 kb on + strand, within B158DRAFT_2288 at 453.406 kb on + strand, within B158DRAFT_2288 at 453.415 kb on + strand, within B158DRAFT_2288 at 453.415 kb on + strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.435 kb on - strand, within B158DRAFT_2288 at 453.467 kb on - strand, within B158DRAFT_2288 at 453.487 kb on + strand, within B158DRAFT_2288 at 453.572 kb on - strand, within B158DRAFT_2288 at 453.779 kb on - strand, within B158DRAFT_2288 at 453.790 kb on + strand, within B158DRAFT_2288 at 453.790 kb on + strand, within B158DRAFT_2288 at 453.798 kb on - strand, within B158DRAFT_2288 at 453.803 kb on - strand, within B158DRAFT_2288
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 451,156 - +0.3 451,158 + +0.3 451,166 - -0.2 451,166 - +0.4 451,166 - -0.3 451,178 - -0.5 451,318 - -0.7 451,318 - -0.1 451,346 - -0.4 451,409 + -0.2 451,431 + +0.1 451,470 + +0.3 451,475 + +0.2 451,478 - -0.0 451,478 - -0.4 451,490 + +0.0 451,496 + +0.9 451,498 - -0.4 451,576 + -0.5 451,591 + +0.0 451,599 - -0.5 451,599 - -0.7 451,806 + -0.0 451,806 + +0.2 451,806 + -0.5 451,842 - +1.3 451,842 - -0.0 451,888 + -0.5 451,912 - +0.5 452,082 + B158DRAFT_2287 0.13 -1.1 452,118 + B158DRAFT_2287 0.17 +0.5 452,118 + B158DRAFT_2287 0.17 -0.4 452,166 - B158DRAFT_2287 0.23 +0.2 452,203 + B158DRAFT_2287 0.27 +0.4 452,211 - B158DRAFT_2287 0.28 -1.1 452,228 + B158DRAFT_2287 0.30 +0.0 452,259 + B158DRAFT_2287 0.34 -0.2 452,259 + B158DRAFT_2287 0.34 -1.1 452,259 + B158DRAFT_2287 0.34 -0.5 452,289 - B158DRAFT_2287 0.37 -0.1 452,369 + B158DRAFT_2287 0.47 -0.3 452,536 - B158DRAFT_2287 0.67 +0.2 452,583 + B158DRAFT_2287 0.72 -0.8 452,583 + B158DRAFT_2287 0.72 -0.1 452,583 + B158DRAFT_2287 0.72 +0.6 452,585 + B158DRAFT_2287 0.72 -1.0 452,591 - B158DRAFT_2287 0.73 +1.1 452,592 - B158DRAFT_2287 0.73 -0.2 452,623 + B158DRAFT_2287 0.77 +0.1 452,623 + B158DRAFT_2287 0.77 -0.0 452,623 + B158DRAFT_2287 0.77 -0.5 452,623 + B158DRAFT_2287 0.77 -0.6 452,623 + B158DRAFT_2287 0.77 -0.2 452,623 + B158DRAFT_2287 0.77 -0.2 452,631 - B158DRAFT_2287 0.78 +0.5 452,631 - B158DRAFT_2287 0.78 -0.1 452,646 + B158DRAFT_2287 0.80 -0.2 452,700 + B158DRAFT_2287 0.86 -0.8 452,832 + -0.2 452,832 + -0.1 452,832 + +0.1 452,832 + -1.0 452,883 + +0.4 452,916 + -0.1 452,916 + -0.3 452,947 - +0.1 452,953 + -0.1 452,953 + +0.0 452,958 + +0.2 452,958 + +0.2 452,958 + +0.2 452,962 + +0.6 452,966 - +0.2 453,018 + B158DRAFT_2288 0.10 +0.0 453,075 + B158DRAFT_2288 0.14 -0.3 453,075 + B158DRAFT_2288 0.14 -0.2 453,083 - B158DRAFT_2288 0.14 +0.1 453,083 - B158DRAFT_2288 0.14 +0.0 453,132 + B158DRAFT_2288 0.17 +0.1 453,140 - B158DRAFT_2288 0.17 +0.5 453,168 - B158DRAFT_2288 0.19 -0.1 453,205 + B158DRAFT_2288 0.21 +0.1 453,250 + B158DRAFT_2288 0.24 -0.0 453,262 - B158DRAFT_2288 0.25 +0.2 453,276 + B158DRAFT_2288 0.26 -0.1 453,286 + B158DRAFT_2288 0.26 +0.3 453,294 - B158DRAFT_2288 0.27 -0.5 453,294 - B158DRAFT_2288 0.27 +0.1 453,374 - B158DRAFT_2288 0.31 +0.1 453,406 + B158DRAFT_2288 0.33 -0.4 453,406 + B158DRAFT_2288 0.33 +0.2 453,415 + B158DRAFT_2288 0.34 +0.1 453,415 + B158DRAFT_2288 0.34 -0.1 453,423 - B158DRAFT_2288 0.34 +0.3 453,423 - B158DRAFT_2288 0.34 +2.4 453,423 - B158DRAFT_2288 0.34 +0.1 453,423 - B158DRAFT_2288 0.34 -0.2 453,423 - B158DRAFT_2288 0.34 +1.5 453,435 - B158DRAFT_2288 0.35 -0.2 453,467 - B158DRAFT_2288 0.37 +0.1 453,487 + B158DRAFT_2288 0.38 +0.6 453,572 - B158DRAFT_2288 0.43 -0.0 453,779 - B158DRAFT_2288 0.55 +0.0 453,790 + B158DRAFT_2288 0.56 +0.6 453,790 + B158DRAFT_2288 0.56 -1.5 453,798 - B158DRAFT_2288 0.56 +0.5 453,803 - B158DRAFT_2288 0.57 +0.0
Or see this region's nucleotide sequence