Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2149 and B158DRAFT_2150 are separated by 148 nucleotides B158DRAFT_2150 and B158DRAFT_2152 are separated by 416 nucleotides
B158DRAFT_2149: B158DRAFT_2149 - Dipeptidyl aminopeptidases/acylaminoacyl-peptidases, at 286,179 to 288,437
_2149
B158DRAFT_2150: B158DRAFT_2150 - Glucose/sorbosone dehydrogenases, at 288,586 to 289,671
_2150
B158DRAFT_2152: B158DRAFT_2152 - Predicted metalloendopeptidase, at 290,088 to 292,181
_2152
Position (kb) 288
289
290 Strain fitness (log2 ratio) -1
0
1 at 287.589 kb on + strand, within B158DRAFT_2149 at 287.597 kb on - strand, within B158DRAFT_2149 at 287.630 kb on + strand, within B158DRAFT_2149 at 287.690 kb on + strand, within B158DRAFT_2149 at 287.690 kb on + strand, within B158DRAFT_2149 at 287.698 kb on - strand, within B158DRAFT_2149 at 287.698 kb on - strand, within B158DRAFT_2149 at 287.706 kb on + strand, within B158DRAFT_2149 at 287.742 kb on - strand, within B158DRAFT_2149 at 287.742 kb on - strand, within B158DRAFT_2149 at 287.781 kb on + strand, within B158DRAFT_2149 at 287.800 kb on + strand, within B158DRAFT_2149 at 287.984 kb on - strand, within B158DRAFT_2149 at 287.999 kb on + strand, within B158DRAFT_2149 at 288.112 kb on - strand, within B158DRAFT_2149 at 288.142 kb on - strand, within B158DRAFT_2149 at 288.163 kb on - strand, within B158DRAFT_2149 at 288.168 kb on - strand, within B158DRAFT_2149 at 288.198 kb on - strand, within B158DRAFT_2149 at 288.297 kb on - strand at 288.299 kb on + strand at 288.299 kb on + strand at 288.299 kb on + strand at 288.299 kb on + strand at 288.299 kb on + strand at 288.299 kb on + strand at 288.307 kb on - strand at 288.307 kb on - strand at 288.307 kb on - strand at 288.307 kb on - strand at 288.307 kb on - strand at 288.309 kb on + strand at 288.395 kb on + strand at 288.395 kb on + strand at 288.398 kb on - strand at 288.403 kb on - strand at 288.513 kb on + strand at 288.547 kb on + strand at 288.619 kb on + strand at 288.671 kb on - strand at 288.799 kb on - strand, within B158DRAFT_2150 at 289.142 kb on - strand, within B158DRAFT_2150 at 289.142 kb on - strand, within B158DRAFT_2150 at 289.156 kb on - strand, within B158DRAFT_2150 at 289.218 kb on + strand, within B158DRAFT_2150 at 289.218 kb on + strand, within B158DRAFT_2150 at 289.286 kb on + strand, within B158DRAFT_2150 at 289.292 kb on - strand, within B158DRAFT_2150 at 289.336 kb on - strand, within B158DRAFT_2150 at 289.336 kb on - strand, within B158DRAFT_2150 at 289.338 kb on + strand, within B158DRAFT_2150 at 289.399 kb on + strand, within B158DRAFT_2150 at 289.442 kb on + strand, within B158DRAFT_2150 at 289.515 kb on + strand, within B158DRAFT_2150 at 289.525 kb on + strand, within B158DRAFT_2150 at 289.533 kb on - strand, within B158DRAFT_2150 at 289.564 kb on + strand at 289.564 kb on + strand at 289.572 kb on - strand at 289.572 kb on - strand at 289.572 kb on - strand at 289.572 kb on - strand at 289.638 kb on + strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.646 kb on - strand at 289.651 kb on - strand at 290.196 kb on + strand at 290.209 kb on - strand at 290.305 kb on + strand, within B158DRAFT_2152 at 290.377 kb on - strand, within B158DRAFT_2152 at 290.416 kb on + strand, within B158DRAFT_2152 at 290.431 kb on + strand, within B158DRAFT_2152 at 290.441 kb on - strand, within B158DRAFT_2152 at 290.605 kb on + strand, within B158DRAFT_2152
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 287,589 + B158DRAFT_2149 0.62 +0.3 287,597 - B158DRAFT_2149 0.63 +0.4 287,630 + B158DRAFT_2149 0.64 +0.8 287,690 + B158DRAFT_2149 0.67 -0.6 287,690 + B158DRAFT_2149 0.67 +0.1 287,698 - B158DRAFT_2149 0.67 -1.7 287,698 - B158DRAFT_2149 0.67 +0.5 287,706 + B158DRAFT_2149 0.68 -0.4 287,742 - B158DRAFT_2149 0.69 +0.1 287,742 - B158DRAFT_2149 0.69 +0.6 287,781 + B158DRAFT_2149 0.71 +0.4 287,800 + B158DRAFT_2149 0.72 -0.0 287,984 - B158DRAFT_2149 0.80 -0.3 287,999 + B158DRAFT_2149 0.81 +0.1 288,112 - B158DRAFT_2149 0.86 -0.0 288,142 - B158DRAFT_2149 0.87 -0.1 288,163 - B158DRAFT_2149 0.88 +1.3 288,168 - B158DRAFT_2149 0.88 -0.2 288,198 - B158DRAFT_2149 0.89 -0.5 288,297 - +0.1 288,299 + -1.1 288,299 + -0.3 288,299 + -0.4 288,299 + -0.3 288,299 + -0.3 288,299 + +0.0 288,307 - -0.4 288,307 - -0.5 288,307 - -0.7 288,307 - +0.1 288,307 - -0.9 288,309 + -0.0 288,395 + +0.3 288,395 + -0.4 288,398 - +0.1 288,403 - -0.2 288,513 + -0.3 288,547 + -0.9 288,619 + +1.1 288,671 - +0.2 288,799 - B158DRAFT_2150 0.20 +0.3 289,142 - B158DRAFT_2150 0.51 -0.5 289,142 - B158DRAFT_2150 0.51 -0.6 289,156 - B158DRAFT_2150 0.52 +0.3 289,218 + B158DRAFT_2150 0.58 +0.0 289,218 + B158DRAFT_2150 0.58 +0.3 289,286 + B158DRAFT_2150 0.64 +0.2 289,292 - B158DRAFT_2150 0.65 -1.0 289,336 - B158DRAFT_2150 0.69 -0.2 289,336 - B158DRAFT_2150 0.69 +0.5 289,338 + B158DRAFT_2150 0.69 -0.7 289,399 + B158DRAFT_2150 0.75 -0.0 289,442 + B158DRAFT_2150 0.79 -0.2 289,515 + B158DRAFT_2150 0.86 +0.4 289,525 + B158DRAFT_2150 0.86 -0.1 289,533 - B158DRAFT_2150 0.87 -0.2 289,564 + -0.7 289,564 + +0.2 289,572 - -0.1 289,572 - -0.5 289,572 - +0.7 289,572 - -0.3 289,638 + -0.1 289,646 - +0.7 289,646 - +0.1 289,646 - +0.9 289,646 - +1.1 289,646 - +0.6 289,651 - +1.1 290,196 + +0.1 290,209 - -0.7 290,305 + B158DRAFT_2152 0.10 -0.5 290,377 - B158DRAFT_2152 0.14 -0.3 290,416 + B158DRAFT_2152 0.16 +1.7 290,431 + B158DRAFT_2152 0.16 -0.3 290,441 - B158DRAFT_2152 0.17 +0.1 290,605 + B158DRAFT_2152 0.25 +0.3
Or see this region's nucleotide sequence