Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2046 and B158DRAFT_2047 are separated by 246 nucleotides B158DRAFT_2047 and B158DRAFT_2048 are separated by 205 nucleotides
B158DRAFT_2046: B158DRAFT_2046 - Zn-dependent alcohol dehydrogenases, at 179,159 to 180,205
_2046
B158DRAFT_2047: B158DRAFT_2047 - ATP-dependent RNA helicase DbpA (EC 5.99.1.-), at 180,452 to 181,834
_2047
B158DRAFT_2048: B158DRAFT_2048 - Uncharacterized conserved protein, at 182,040 to 182,711
_2048
Position (kb)
180
181
182 Strain fitness (log2 ratio)
-2
-1
0
1 at 179.462 kb on + strand, within B158DRAFT_2046 at 179.486 kb on + strand, within B158DRAFT_2046 at 179.494 kb on - strand, within B158DRAFT_2046 at 179.498 kb on + strand, within B158DRAFT_2046 at 179.498 kb on + strand, within B158DRAFT_2046 at 179.506 kb on - strand, within B158DRAFT_2046 at 179.506 kb on - strand, within B158DRAFT_2046 at 179.506 kb on - strand, within B158DRAFT_2046 at 179.533 kb on + strand, within B158DRAFT_2046 at 179.621 kb on + strand, within B158DRAFT_2046 at 179.629 kb on - strand, within B158DRAFT_2046 at 179.727 kb on + strand, within B158DRAFT_2046 at 179.735 kb on - strand, within B158DRAFT_2046 at 179.737 kb on + strand, within B158DRAFT_2046 at 179.737 kb on + strand, within B158DRAFT_2046 at 179.737 kb on + strand, within B158DRAFT_2046 at 179.740 kb on - strand, within B158DRAFT_2046 at 179.751 kb on + strand, within B158DRAFT_2046 at 179.788 kb on + strand, within B158DRAFT_2046 at 179.796 kb on - strand, within B158DRAFT_2046 at 179.796 kb on - strand, within B158DRAFT_2046 at 179.830 kb on - strand, within B158DRAFT_2046 at 179.876 kb on - strand, within B158DRAFT_2046 at 179.915 kb on - strand, within B158DRAFT_2046 at 179.938 kb on + strand, within B158DRAFT_2046 at 179.975 kb on + strand, within B158DRAFT_2046 at 180.004 kb on - strand, within B158DRAFT_2046 at 180.024 kb on - strand, within B158DRAFT_2046 at 180.124 kb on - strand at 180.212 kb on - strand at 180.227 kb on - strand at 180.227 kb on - strand at 180.233 kb on + strand at 180.233 kb on + strand at 180.241 kb on - strand at 180.243 kb on + strand at 180.246 kb on - strand at 180.246 kb on - strand at 180.246 kb on - strand at 180.329 kb on + strand at 180.329 kb on + strand at 180.329 kb on + strand at 180.329 kb on + strand at 180.329 kb on + strand at 180.337 kb on - strand at 180.337 kb on - strand at 180.337 kb on - strand at 180.337 kb on - strand at 180.337 kb on - strand at 180.337 kb on - strand at 180.345 kb on - strand at 180.348 kb on + strand at 180.356 kb on - strand at 180.358 kb on + strand at 180.383 kb on + strand at 180.391 kb on - strand at 180.391 kb on - strand at 180.391 kb on - strand at 180.391 kb on - strand at 180.391 kb on - strand at 180.391 kb on - strand at 180.393 kb on + strand at 180.393 kb on + strand at 180.393 kb on + strand at 180.393 kb on + strand at 180.393 kb on + strand at 180.401 kb on - strand at 180.401 kb on - strand at 180.401 kb on - strand at 180.401 kb on - strand at 180.401 kb on - strand at 180.421 kb on - strand at 180.487 kb on + strand at 180.512 kb on + strand at 180.512 kb on + strand at 180.572 kb on + strand at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.592 kb on + strand, within B158DRAFT_2047 at 180.600 kb on - strand, within B158DRAFT_2047 at 180.600 kb on - strand, within B158DRAFT_2047 at 180.600 kb on - strand, within B158DRAFT_2047 at 180.600 kb on - strand, within B158DRAFT_2047 at 180.600 kb on - strand, within B158DRAFT_2047 at 180.600 kb on - strand, within B158DRAFT_2047 at 180.682 kb on + strand, within B158DRAFT_2047 at 180.685 kb on - strand, within B158DRAFT_2047 at 180.727 kb on - strand, within B158DRAFT_2047 at 180.762 kb on - strand, within B158DRAFT_2047 at 180.777 kb on + strand, within B158DRAFT_2047 at 180.779 kb on + strand, within B158DRAFT_2047 at 180.779 kb on + strand, within B158DRAFT_2047 at 180.779 kb on + strand, within B158DRAFT_2047 at 180.785 kb on + strand, within B158DRAFT_2047 at 180.785 kb on + strand, within B158DRAFT_2047 at 180.793 kb on - strand, within B158DRAFT_2047 at 180.793 kb on - strand, within B158DRAFT_2047 at 180.793 kb on - strand, within B158DRAFT_2047 at 180.807 kb on + strand, within B158DRAFT_2047 at 180.849 kb on + strand, within B158DRAFT_2047 at 180.857 kb on - strand, within B158DRAFT_2047 at 180.908 kb on - strand, within B158DRAFT_2047 at 180.910 kb on + strand, within B158DRAFT_2047 at 180.920 kb on + strand, within B158DRAFT_2047 at 180.950 kb on + strand, within B158DRAFT_2047 at 180.995 kb on + strand, within B158DRAFT_2047 at 181.003 kb on - strand, within B158DRAFT_2047 at 181.003 kb on - strand, within B158DRAFT_2047 at 181.003 kb on - strand, within B158DRAFT_2047 at 181.003 kb on - strand, within B158DRAFT_2047 at 181.003 kb on - strand, within B158DRAFT_2047 at 181.014 kb on + strand, within B158DRAFT_2047 at 181.022 kb on - strand, within B158DRAFT_2047 at 181.022 kb on - strand, within B158DRAFT_2047 at 181.085 kb on + strand, within B158DRAFT_2047 at 181.093 kb on + strand, within B158DRAFT_2047 at 181.101 kb on - strand, within B158DRAFT_2047 at 181.103 kb on + strand, within B158DRAFT_2047 at 181.304 kb on + strand, within B158DRAFT_2047 at 181.347 kb on + strand, within B158DRAFT_2047 at 181.412 kb on - strand, within B158DRAFT_2047 at 181.419 kb on + strand, within B158DRAFT_2047 at 181.419 kb on + strand, within B158DRAFT_2047 at 181.419 kb on + strand, within B158DRAFT_2047 at 181.427 kb on + strand, within B158DRAFT_2047 at 181.427 kb on - strand, within B158DRAFT_2047 at 181.427 kb on - strand, within B158DRAFT_2047 at 181.444 kb on + strand, within B158DRAFT_2047 at 181.444 kb on + strand, within B158DRAFT_2047 at 181.472 kb on + strand, within B158DRAFT_2047 at 181.472 kb on + strand, within B158DRAFT_2047 at 181.490 kb on - strand, within B158DRAFT_2047 at 181.663 kb on - strand, within B158DRAFT_2047 at 181.729 kb on + strand at 181.929 kb on + strand at 181.930 kb on + strand at 181.938 kb on - strand at 181.969 kb on - strand at 182.106 kb on - strand at 182.110 kb on + strand, within B158DRAFT_2048 at 182.110 kb on + strand, within B158DRAFT_2048 at 182.115 kb on + strand, within B158DRAFT_2048 at 182.249 kb on - strand, within B158DRAFT_2048 at 182.273 kb on + strand, within B158DRAFT_2048 at 182.372 kb on - strand, within B158DRAFT_2048 at 182.373 kb on - strand, within B158DRAFT_2048 at 182.383 kb on - strand, within B158DRAFT_2048 at 182.438 kb on + strand, within B158DRAFT_2048 at 182.438 kb on + strand, within B158DRAFT_2048 at 182.438 kb on + strand, within B158DRAFT_2048 at 182.441 kb on - strand, within B158DRAFT_2048 at 182.446 kb on - strand, within B158DRAFT_2048 at 182.446 kb on - strand, within B158DRAFT_2048 at 182.446 kb on - strand, within B158DRAFT_2048 at 182.457 kb on + strand, within B158DRAFT_2048 at 182.483 kb on + strand, within B158DRAFT_2048 at 182.483 kb on + strand, within B158DRAFT_2048 at 182.504 kb on + strand, within B158DRAFT_2048 at 182.504 kb on + strand, within B158DRAFT_2048 at 182.512 kb on - strand, within B158DRAFT_2048 at 182.512 kb on - strand, within B158DRAFT_2048 at 182.512 kb on - strand, within B158DRAFT_2048 at 182.606 kb on + strand, within B158DRAFT_2048 at 182.606 kb on + strand, within B158DRAFT_2048 at 182.606 kb on + strand, within B158DRAFT_2048 at 182.606 kb on + strand, within B158DRAFT_2048 at 182.614 kb on - strand, within B158DRAFT_2048 at 182.614 kb on - strand, within B158DRAFT_2048 at 182.614 kb on - strand, within B158DRAFT_2048 at 182.614 kb on - strand, within B158DRAFT_2048 at 182.647 kb on + strand at 182.671 kb on + strand at 182.725 kb on - strand at 182.735 kb on - strand at 182.770 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 179,462 + B158DRAFT_2046 0.29 +0.9 179,486 + B158DRAFT_2046 0.31 -0.8 179,494 - B158DRAFT_2046 0.32 +0.3 179,498 + B158DRAFT_2046 0.32 +0.3 179,498 + B158DRAFT_2046 0.32 -0.2 179,506 - B158DRAFT_2046 0.33 +0.3 179,506 - B158DRAFT_2046 0.33 -1.0 179,506 - B158DRAFT_2046 0.33 +0.0 179,533 + B158DRAFT_2046 0.36 +0.5 179,621 + B158DRAFT_2046 0.44 -0.1 179,629 - B158DRAFT_2046 0.45 +0.2 179,727 + B158DRAFT_2046 0.54 -0.3 179,735 - B158DRAFT_2046 0.55 -0.3 179,737 + B158DRAFT_2046 0.55 -0.8 179,737 + B158DRAFT_2046 0.55 -0.1 179,737 + B158DRAFT_2046 0.55 +0.6 179,740 - B158DRAFT_2046 0.55 -1.1 179,751 + B158DRAFT_2046 0.57 -0.6 179,788 + B158DRAFT_2046 0.60 -0.9 179,796 - B158DRAFT_2046 0.61 -0.0 179,796 - B158DRAFT_2046 0.61 -0.7 179,830 - B158DRAFT_2046 0.64 -0.4 179,876 - B158DRAFT_2046 0.68 +0.2 179,915 - B158DRAFT_2046 0.72 -0.8 179,938 + B158DRAFT_2046 0.74 +0.0 179,975 + B158DRAFT_2046 0.78 +0.3 180,004 - B158DRAFT_2046 0.81 -0.1 180,024 - B158DRAFT_2046 0.83 -0.1 180,124 - +0.6 180,212 - +0.7 180,227 - -0.3 180,227 - -0.1 180,233 + -0.7 180,233 + -0.6 180,241 - -1.6 180,243 + -0.3 180,246 - +0.1 180,246 - +0.1 180,246 - +0.1 180,329 + -0.5 180,329 + -0.4 180,329 + -0.0 180,329 + -0.2 180,329 + -0.7 180,337 - +0.6 180,337 - +0.3 180,337 - -0.0 180,337 - -0.5 180,337 - -0.9 180,337 - +0.3 180,345 - -0.1 180,348 + -0.3 180,356 - +0.5 180,358 + +0.9 180,383 + +0.2 180,391 - -0.1 180,391 - +0.7 180,391 - -0.1 180,391 - +0.4 180,391 - -0.5 180,391 - -0.5 180,393 + +0.5 180,393 + -0.0 180,393 + +0.4 180,393 + +0.1 180,393 + +0.0 180,401 - +0.3 180,401 - -0.3 180,401 - -0.4 180,401 - +0.7 180,401 - +0.3 180,421 - -0.6 180,487 + +0.3 180,512 + -0.6 180,512 + -0.2 180,572 + -0.3 180,592 + B158DRAFT_2047 0.10 +0.3 180,592 + B158DRAFT_2047 0.10 -0.8 180,592 + B158DRAFT_2047 0.10 +0.1 180,592 + B158DRAFT_2047 0.10 -0.2 180,592 + B158DRAFT_2047 0.10 +0.1 180,592 + B158DRAFT_2047 0.10 +0.3 180,592 + B158DRAFT_2047 0.10 +0.1 180,592 + B158DRAFT_2047 0.10 +0.0 180,592 + B158DRAFT_2047 0.10 -0.9 180,592 + B158DRAFT_2047 0.10 -0.6 180,600 - B158DRAFT_2047 0.11 -1.3 180,600 - B158DRAFT_2047 0.11 -0.0 180,600 - B158DRAFT_2047 0.11 +0.1 180,600 - B158DRAFT_2047 0.11 +0.0 180,600 - B158DRAFT_2047 0.11 +0.2 180,600 - B158DRAFT_2047 0.11 -0.1 180,682 + B158DRAFT_2047 0.17 -0.6 180,685 - B158DRAFT_2047 0.17 -0.0 180,727 - B158DRAFT_2047 0.20 -1.2 180,762 - B158DRAFT_2047 0.22 +0.1 180,777 + B158DRAFT_2047 0.23 -0.2 180,779 + B158DRAFT_2047 0.24 -0.1 180,779 + B158DRAFT_2047 0.24 +0.3 180,779 + B158DRAFT_2047 0.24 +0.1 180,785 + B158DRAFT_2047 0.24 +0.2 180,785 + B158DRAFT_2047 0.24 +0.6 180,793 - B158DRAFT_2047 0.25 -0.1 180,793 - B158DRAFT_2047 0.25 +0.3 180,793 - B158DRAFT_2047 0.25 -0.1 180,807 + B158DRAFT_2047 0.26 +0.2 180,849 + B158DRAFT_2047 0.29 +1.6 180,857 - B158DRAFT_2047 0.29 +0.1 180,908 - B158DRAFT_2047 0.33 +0.2 180,910 + B158DRAFT_2047 0.33 -0.3 180,920 + B158DRAFT_2047 0.34 +0.4 180,950 + B158DRAFT_2047 0.36 -0.3 180,995 + B158DRAFT_2047 0.39 -0.0 181,003 - B158DRAFT_2047 0.40 -0.6 181,003 - B158DRAFT_2047 0.40 -0.2 181,003 - B158DRAFT_2047 0.40 -0.1 181,003 - B158DRAFT_2047 0.40 -0.4 181,003 - B158DRAFT_2047 0.40 -0.3 181,014 + B158DRAFT_2047 0.41 -0.6 181,022 - B158DRAFT_2047 0.41 +1.5 181,022 - B158DRAFT_2047 0.41 +0.3 181,085 + B158DRAFT_2047 0.46 -0.3 181,093 + B158DRAFT_2047 0.46 +0.1 181,101 - B158DRAFT_2047 0.47 -0.1 181,103 + B158DRAFT_2047 0.47 -0.1 181,304 + B158DRAFT_2047 0.62 -0.5 181,347 + B158DRAFT_2047 0.65 +0.5 181,412 - B158DRAFT_2047 0.69 -0.4 181,419 + B158DRAFT_2047 0.70 +0.1 181,419 + B158DRAFT_2047 0.70 -0.1 181,419 + B158DRAFT_2047 0.70 +0.2 181,427 + B158DRAFT_2047 0.70 +0.4 181,427 - B158DRAFT_2047 0.70 -0.5 181,427 - B158DRAFT_2047 0.70 +0.3 181,444 + B158DRAFT_2047 0.72 -0.0 181,444 + B158DRAFT_2047 0.72 -0.1 181,472 + B158DRAFT_2047 0.74 -0.5 181,472 + B158DRAFT_2047 0.74 -0.4 181,490 - B158DRAFT_2047 0.75 -0.9 181,663 - B158DRAFT_2047 0.88 +0.1 181,729 + -0.1 181,929 + +0.6 181,930 + -0.3 181,938 - -0.6 181,969 - -0.7 182,106 - +0.4 182,110 + B158DRAFT_2048 0.10 +0.5 182,110 + B158DRAFT_2048 0.10 +0.3 182,115 + B158DRAFT_2048 0.11 -0.5 182,249 - B158DRAFT_2048 0.31 -0.5 182,273 + B158DRAFT_2048 0.35 -0.0 182,372 - B158DRAFT_2048 0.49 +0.6 182,373 - B158DRAFT_2048 0.50 -0.5 182,383 - B158DRAFT_2048 0.51 -0.6 182,438 + B158DRAFT_2048 0.59 -0.4 182,438 + B158DRAFT_2048 0.59 +0.5 182,438 + B158DRAFT_2048 0.59 -0.7 182,441 - B158DRAFT_2048 0.60 -0.6 182,446 - B158DRAFT_2048 0.60 -0.5 182,446 - B158DRAFT_2048 0.60 -0.5 182,446 - B158DRAFT_2048 0.60 -0.4 182,457 + B158DRAFT_2048 0.62 +0.1 182,483 + B158DRAFT_2048 0.66 -0.3 182,483 + B158DRAFT_2048 0.66 -0.4 182,504 + B158DRAFT_2048 0.69 -0.0 182,504 + B158DRAFT_2048 0.69 -0.5 182,512 - B158DRAFT_2048 0.70 -0.3 182,512 - B158DRAFT_2048 0.70 +0.3 182,512 - B158DRAFT_2048 0.70 +0.7 182,606 + B158DRAFT_2048 0.84 -1.9 182,606 + B158DRAFT_2048 0.84 -0.4 182,606 + B158DRAFT_2048 0.84 +0.7 182,606 + B158DRAFT_2048 0.84 +0.5 182,614 - B158DRAFT_2048 0.85 +0.1 182,614 - B158DRAFT_2048 0.85 +0.0 182,614 - B158DRAFT_2048 0.85 -0.5 182,614 - B158DRAFT_2048 0.85 -1.4 182,647 + -0.8 182,671 + +0.0 182,725 - -0.7 182,735 - +0.1 182,770 - -0.7
Or see this region's nucleotide sequence