Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1832 and B158DRAFT_1833 are separated by 107 nucleotides B158DRAFT_1833 and B158DRAFT_1834 are separated by 220 nucleotides B158DRAFT_1834 and B158DRAFT_1835 overlap by 1 nucleotides
B158DRAFT_1832: B158DRAFT_1832 - L-threonine aldolase (EC 4.1.2.5), at 1,930,395 to 1,931,402
_1832
B158DRAFT_1833: B158DRAFT_1833 - Uncharacterized flavoproteins, at 1,931,510 to 1,932,322
_1833
B158DRAFT_1834: B158DRAFT_1834 - SOS-response transcriptional repressors (RecA-mediated autopeptidases), at 1,932,543 to 1,932,965
_1834
B158DRAFT_1835: B158DRAFT_1835 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 1,932,965 to 1,934,233
_1835
Position (kb)
1931
1932
1933 Strain fitness (log2 ratio)
-2
-1
0
1 at 1930.529 kb on + strand, within B158DRAFT_1832 at 1930.537 kb on - strand, within B158DRAFT_1832 at 1930.562 kb on + strand, within B158DRAFT_1832 at 1930.674 kb on - strand, within B158DRAFT_1832 at 1930.686 kb on + strand, within B158DRAFT_1832 at 1930.729 kb on - strand, within B158DRAFT_1832 at 1930.782 kb on - strand, within B158DRAFT_1832 at 1930.782 kb on - strand, within B158DRAFT_1832 at 1930.784 kb on + strand, within B158DRAFT_1832 at 1930.784 kb on + strand, within B158DRAFT_1832 at 1930.784 kb on + strand, within B158DRAFT_1832 at 1930.791 kb on + strand, within B158DRAFT_1832 at 1930.791 kb on + strand, within B158DRAFT_1832 at 1930.792 kb on - strand, within B158DRAFT_1832 at 1930.792 kb on - strand, within B158DRAFT_1832 at 1930.792 kb on - strand, within B158DRAFT_1832 at 1930.799 kb on - strand, within B158DRAFT_1832 at 1930.811 kb on + strand, within B158DRAFT_1832 at 1930.832 kb on - strand, within B158DRAFT_1832 at 1930.854 kb on + strand, within B158DRAFT_1832 at 1930.869 kb on + strand, within B158DRAFT_1832 at 1930.870 kb on - strand, within B158DRAFT_1832 at 1930.870 kb on - strand, within B158DRAFT_1832 at 1930.870 kb on - strand, within B158DRAFT_1832 at 1930.870 kb on - strand, within B158DRAFT_1832 at 1930.885 kb on - strand, within B158DRAFT_1832 at 1930.886 kb on + strand, within B158DRAFT_1832 at 1930.913 kb on - strand, within B158DRAFT_1832 at 1930.961 kb on + strand, within B158DRAFT_1832 at 1930.969 kb on + strand, within B158DRAFT_1832 at 1930.976 kb on - strand, within B158DRAFT_1832 at 1931.058 kb on - strand, within B158DRAFT_1832 at 1931.067 kb on + strand, within B158DRAFT_1832 at 1931.067 kb on + strand, within B158DRAFT_1832 at 1931.074 kb on + strand, within B158DRAFT_1832 at 1931.075 kb on - strand, within B158DRAFT_1832 at 1931.075 kb on - strand, within B158DRAFT_1832 at 1931.075 kb on - strand, within B158DRAFT_1832 at 1931.075 kb on - strand, within B158DRAFT_1832 at 1931.075 kb on - strand, within B158DRAFT_1832 at 1931.092 kb on + strand, within B158DRAFT_1832 at 1931.105 kb on + strand, within B158DRAFT_1832 at 1931.105 kb on + strand, within B158DRAFT_1832 at 1931.113 kb on - strand, within B158DRAFT_1832 at 1931.115 kb on + strand, within B158DRAFT_1832 at 1931.115 kb on + strand, within B158DRAFT_1832 at 1931.115 kb on + strand, within B158DRAFT_1832 at 1931.115 kb on + strand, within B158DRAFT_1832 at 1931.115 kb on + strand, within B158DRAFT_1832 at 1931.121 kb on - strand, within B158DRAFT_1832 at 1931.123 kb on - strand, within B158DRAFT_1832 at 1931.123 kb on - strand, within B158DRAFT_1832 at 1931.123 kb on - strand, within B158DRAFT_1832 at 1931.123 kb on - strand, within B158DRAFT_1832 at 1931.127 kb on + strand, within B158DRAFT_1832 at 1931.145 kb on - strand, within B158DRAFT_1832 at 1931.170 kb on + strand, within B158DRAFT_1832 at 1931.185 kb on - strand, within B158DRAFT_1832 at 1931.188 kb on + strand, within B158DRAFT_1832 at 1931.287 kb on + strand, within B158DRAFT_1832 at 1931.328 kb on - strand at 1931.335 kb on + strand at 1931.335 kb on + strand at 1931.343 kb on - strand at 1931.355 kb on - strand at 1931.373 kb on - strand at 1931.378 kb on + strand at 1931.378 kb on + strand at 1931.424 kb on + strand at 1931.424 kb on + strand at 1931.424 kb on + strand at 1931.424 kb on + strand at 1931.424 kb on + strand at 1931.432 kb on - strand at 1931.432 kb on - strand at 1931.507 kb on + strand at 1931.507 kb on + strand at 1931.507 kb on + strand at 1931.508 kb on + strand at 1931.515 kb on - strand at 1931.515 kb on - strand at 1931.515 kb on - strand at 1931.515 kb on - strand at 1931.541 kb on + strand at 1931.541 kb on + strand at 1931.558 kb on + strand at 1931.564 kb on + strand at 1931.605 kb on + strand, within B158DRAFT_1833 at 1931.605 kb on + strand, within B158DRAFT_1833 at 1931.610 kb on + strand, within B158DRAFT_1833 at 1931.723 kb on - strand, within B158DRAFT_1833 at 1931.723 kb on - strand, within B158DRAFT_1833 at 1931.723 kb on - strand, within B158DRAFT_1833 at 1931.733 kb on - strand, within B158DRAFT_1833 at 1931.772 kb on + strand, within B158DRAFT_1833 at 1931.772 kb on + strand, within B158DRAFT_1833 at 1931.807 kb on + strand, within B158DRAFT_1833 at 1931.822 kb on - strand, within B158DRAFT_1833 at 1931.822 kb on - strand, within B158DRAFT_1833 at 1931.822 kb on - strand, within B158DRAFT_1833 at 1931.879 kb on + strand, within B158DRAFT_1833 at 1931.887 kb on - strand, within B158DRAFT_1833 at 1931.919 kb on - strand, within B158DRAFT_1833 at 1931.922 kb on - strand, within B158DRAFT_1833 at 1931.927 kb on - strand, within B158DRAFT_1833 at 1931.932 kb on - strand, within B158DRAFT_1833 at 1932.007 kb on - strand, within B158DRAFT_1833 at 1932.007 kb on - strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.022 kb on + strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.030 kb on - strand, within B158DRAFT_1833 at 1932.150 kb on + strand, within B158DRAFT_1833 at 1932.162 kb on + strand, within B158DRAFT_1833 at 1932.192 kb on - strand, within B158DRAFT_1833 at 1932.198 kb on + strand, within B158DRAFT_1833 at 1932.238 kb on - strand, within B158DRAFT_1833 at 1932.249 kb on + strand at 1932.281 kb on - strand at 1932.324 kb on + strand at 1932.367 kb on - strand at 1932.461 kb on + strand at 1932.591 kb on + strand, within B158DRAFT_1834 at 1932.591 kb on + strand, within B158DRAFT_1834 at 1932.591 kb on + strand, within B158DRAFT_1834 at 1932.591 kb on + strand, within B158DRAFT_1834 at 1932.599 kb on - strand, within B158DRAFT_1834 at 1932.599 kb on - strand, within B158DRAFT_1834 at 1932.599 kb on - strand, within B158DRAFT_1834 at 1932.639 kb on - strand, within B158DRAFT_1834 at 1932.651 kb on + strand, within B158DRAFT_1834 at 1932.669 kb on - strand, within B158DRAFT_1834 at 1932.890 kb on - strand, within B158DRAFT_1834 at 1932.893 kb on + strand, within B158DRAFT_1834 at 1932.904 kb on - strand, within B158DRAFT_1834 at 1932.928 kb on + strand at 1932.984 kb on + strand at 1932.984 kb on + strand at 1932.984 kb on + strand at 1932.984 kb on + strand at 1932.984 kb on + strand at 1932.992 kb on - strand at 1932.992 kb on - strand at 1932.992 kb on - strand at 1932.992 kb on - strand at 1932.992 kb on - strand at 1933.002 kb on - strand at 1933.016 kb on - strand at 1933.016 kb on - strand at 1933.092 kb on + strand, within B158DRAFT_1835 at 1933.127 kb on + strand, within B158DRAFT_1835 at 1933.182 kb on - strand, within B158DRAFT_1835 at 1933.200 kb on + strand, within B158DRAFT_1835 at 1933.208 kb on - strand, within B158DRAFT_1835 at 1933.268 kb on + strand, within B158DRAFT_1835 at 1933.320 kb on + strand, within B158DRAFT_1835 at 1933.320 kb on + strand, within B158DRAFT_1835 at 1933.320 kb on + strand, within B158DRAFT_1835
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 1,930,529 + B158DRAFT_1832 0.13 -0.2 1,930,537 - B158DRAFT_1832 0.14 -0.2 1,930,562 + B158DRAFT_1832 0.17 -0.5 1,930,674 - B158DRAFT_1832 0.28 -0.5 1,930,686 + B158DRAFT_1832 0.29 +0.3 1,930,729 - B158DRAFT_1832 0.33 -0.2 1,930,782 - B158DRAFT_1832 0.38 -0.3 1,930,782 - B158DRAFT_1832 0.38 -0.9 1,930,784 + B158DRAFT_1832 0.39 +0.4 1,930,784 + B158DRAFT_1832 0.39 +0.1 1,930,784 + B158DRAFT_1832 0.39 +1.0 1,930,791 + B158DRAFT_1832 0.39 -0.1 1,930,791 + B158DRAFT_1832 0.39 -0.5 1,930,792 - B158DRAFT_1832 0.39 -2.5 1,930,792 - B158DRAFT_1832 0.39 -0.5 1,930,792 - B158DRAFT_1832 0.39 -0.2 1,930,799 - B158DRAFT_1832 0.40 -0.3 1,930,811 + B158DRAFT_1832 0.41 +0.2 1,930,832 - B158DRAFT_1832 0.43 -0.1 1,930,854 + B158DRAFT_1832 0.46 -0.2 1,930,869 + B158DRAFT_1832 0.47 -0.9 1,930,870 - B158DRAFT_1832 0.47 -0.8 1,930,870 - B158DRAFT_1832 0.47 +0.1 1,930,870 - B158DRAFT_1832 0.47 +0.0 1,930,870 - B158DRAFT_1832 0.47 -0.0 1,930,885 - B158DRAFT_1832 0.49 +0.5 1,930,886 + B158DRAFT_1832 0.49 +0.4 1,930,913 - B158DRAFT_1832 0.51 -0.2 1,930,961 + B158DRAFT_1832 0.56 -0.6 1,930,969 + B158DRAFT_1832 0.57 -0.1 1,930,976 - B158DRAFT_1832 0.58 -0.3 1,931,058 - B158DRAFT_1832 0.66 -0.3 1,931,067 + B158DRAFT_1832 0.67 -0.0 1,931,067 + B158DRAFT_1832 0.67 -0.6 1,931,074 + B158DRAFT_1832 0.67 -0.8 1,931,075 - B158DRAFT_1832 0.67 -1.3 1,931,075 - B158DRAFT_1832 0.67 +0.1 1,931,075 - B158DRAFT_1832 0.67 -0.0 1,931,075 - B158DRAFT_1832 0.67 -0.5 1,931,075 - B158DRAFT_1832 0.67 -1.9 1,931,092 + B158DRAFT_1832 0.69 -0.6 1,931,105 + B158DRAFT_1832 0.70 -0.8 1,931,105 + B158DRAFT_1832 0.70 -0.3 1,931,113 - B158DRAFT_1832 0.71 -0.1 1,931,115 + B158DRAFT_1832 0.71 -0.2 1,931,115 + B158DRAFT_1832 0.71 -0.5 1,931,115 + B158DRAFT_1832 0.71 -0.5 1,931,115 + B158DRAFT_1832 0.71 -0.3 1,931,115 + B158DRAFT_1832 0.71 -0.9 1,931,121 - B158DRAFT_1832 0.72 -0.9 1,931,123 - B158DRAFT_1832 0.72 -0.1 1,931,123 - B158DRAFT_1832 0.72 -0.3 1,931,123 - B158DRAFT_1832 0.72 +0.2 1,931,123 - B158DRAFT_1832 0.72 -0.3 1,931,127 + B158DRAFT_1832 0.73 +0.2 1,931,145 - B158DRAFT_1832 0.74 -0.9 1,931,170 + B158DRAFT_1832 0.77 -0.0 1,931,185 - B158DRAFT_1832 0.78 -0.2 1,931,188 + B158DRAFT_1832 0.79 +0.2 1,931,287 + B158DRAFT_1832 0.88 -0.1 1,931,328 - -0.1 1,931,335 + -1.1 1,931,335 + -0.3 1,931,343 - -1.1 1,931,355 - +0.3 1,931,373 - -0.1 1,931,378 + +1.1 1,931,378 + +0.8 1,931,424 + -0.1 1,931,424 + -0.3 1,931,424 + -0.3 1,931,424 + -0.2 1,931,424 + -0.5 1,931,432 - -0.6 1,931,432 - -0.2 1,931,507 + +0.7 1,931,507 + -1.0 1,931,507 + -0.0 1,931,508 + -0.4 1,931,515 - +0.1 1,931,515 - -0.4 1,931,515 - -0.0 1,931,515 - -0.3 1,931,541 + -0.8 1,931,541 + -1.0 1,931,558 + -0.0 1,931,564 + -0.4 1,931,605 + B158DRAFT_1833 0.12 -0.1 1,931,605 + B158DRAFT_1833 0.12 -0.3 1,931,610 + B158DRAFT_1833 0.12 +0.7 1,931,723 - B158DRAFT_1833 0.26 -0.6 1,931,723 - B158DRAFT_1833 0.26 -0.3 1,931,723 - B158DRAFT_1833 0.26 -0.1 1,931,733 - B158DRAFT_1833 0.27 -0.0 1,931,772 + B158DRAFT_1833 0.32 -0.6 1,931,772 + B158DRAFT_1833 0.32 -0.1 1,931,807 + B158DRAFT_1833 0.37 -0.4 1,931,822 - B158DRAFT_1833 0.38 -0.1 1,931,822 - B158DRAFT_1833 0.38 -1.3 1,931,822 - B158DRAFT_1833 0.38 +0.7 1,931,879 + B158DRAFT_1833 0.45 -1.0 1,931,887 - B158DRAFT_1833 0.46 -1.0 1,931,919 - B158DRAFT_1833 0.50 +0.4 1,931,922 - B158DRAFT_1833 0.51 +0.1 1,931,927 - B158DRAFT_1833 0.51 -0.4 1,931,932 - B158DRAFT_1833 0.52 +0.7 1,932,007 - B158DRAFT_1833 0.61 -0.6 1,932,007 - B158DRAFT_1833 0.61 +0.6 1,932,022 + B158DRAFT_1833 0.63 -0.5 1,932,022 + B158DRAFT_1833 0.63 -1.3 1,932,022 + B158DRAFT_1833 0.63 -0.9 1,932,022 + B158DRAFT_1833 0.63 +0.5 1,932,022 + B158DRAFT_1833 0.63 -0.4 1,932,022 + B158DRAFT_1833 0.63 -0.0 1,932,022 + B158DRAFT_1833 0.63 -0.2 1,932,022 + B158DRAFT_1833 0.63 +0.1 1,932,022 + B158DRAFT_1833 0.63 -0.4 1,932,022 + B158DRAFT_1833 0.63 +0.1 1,932,022 + B158DRAFT_1833 0.63 -0.9 1,932,022 + B158DRAFT_1833 0.63 -0.4 1,932,022 + B158DRAFT_1833 0.63 -0.2 1,932,030 - B158DRAFT_1833 0.64 +0.9 1,932,030 - B158DRAFT_1833 0.64 -0.9 1,932,030 - B158DRAFT_1833 0.64 -0.0 1,932,030 - B158DRAFT_1833 0.64 -0.2 1,932,030 - B158DRAFT_1833 0.64 -2.5 1,932,030 - B158DRAFT_1833 0.64 -0.7 1,932,030 - B158DRAFT_1833 0.64 -0.0 1,932,030 - B158DRAFT_1833 0.64 +0.5 1,932,150 + B158DRAFT_1833 0.79 -0.3 1,932,162 + B158DRAFT_1833 0.80 -1.4 1,932,192 - B158DRAFT_1833 0.84 -0.3 1,932,198 + B158DRAFT_1833 0.85 -0.3 1,932,238 - B158DRAFT_1833 0.90 -0.3 1,932,249 + -1.4 1,932,281 - +0.3 1,932,324 + +0.9 1,932,367 - -1.6 1,932,461 + -0.6 1,932,591 + B158DRAFT_1834 0.11 -0.4 1,932,591 + B158DRAFT_1834 0.11 +0.2 1,932,591 + B158DRAFT_1834 0.11 -0.2 1,932,591 + B158DRAFT_1834 0.11 -0.6 1,932,599 - B158DRAFT_1834 0.13 -0.1 1,932,599 - B158DRAFT_1834 0.13 -0.5 1,932,599 - B158DRAFT_1834 0.13 -0.2 1,932,639 - B158DRAFT_1834 0.23 -0.8 1,932,651 + B158DRAFT_1834 0.26 -0.5 1,932,669 - B158DRAFT_1834 0.30 +0.1 1,932,890 - B158DRAFT_1834 0.82 +0.6 1,932,893 + B158DRAFT_1834 0.83 -0.8 1,932,904 - B158DRAFT_1834 0.85 -0.6 1,932,928 + +0.2 1,932,984 + -0.6 1,932,984 + -0.0 1,932,984 + +0.0 1,932,984 + -0.3 1,932,984 + -0.2 1,932,992 - -1.3 1,932,992 - -0.5 1,932,992 - -0.1 1,932,992 - -0.4 1,932,992 - +0.5 1,933,002 - +0.0 1,933,016 - -0.4 1,933,016 - +0.1 1,933,092 + B158DRAFT_1835 0.10 +0.2 1,933,127 + B158DRAFT_1835 0.13 +0.4 1,933,182 - B158DRAFT_1835 0.17 -0.2 1,933,200 + B158DRAFT_1835 0.19 -0.8 1,933,208 - B158DRAFT_1835 0.19 +0.1 1,933,268 + B158DRAFT_1835 0.24 -0.3 1,933,320 + B158DRAFT_1835 0.28 +0.2 1,933,320 + B158DRAFT_1835 0.28 -0.8 1,933,320 + B158DRAFT_1835 0.28 -0.3
Or see this region's nucleotide sequence