Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1143 and B158DRAFT_1144 are separated by 104 nucleotides B158DRAFT_1144 and B158DRAFT_1145 are separated by 56 nucleotides B158DRAFT_1145 and B158DRAFT_1146 are separated by 93 nucleotides B158DRAFT_1146 and B158DRAFT_1147 are separated by 73 nucleotides
B158DRAFT_1143: B158DRAFT_1143 - tol-pal system protein YbgF, at 1,180,866 to 1,181,600
_1143
B158DRAFT_1144: B158DRAFT_1144 - Organic radical activating enzymes, at 1,181,705 to 1,182,346
_1144
B158DRAFT_1145: B158DRAFT_1145 - hypothetical protein, at 1,182,403 to 1,182,510
_1145
B158DRAFT_1146: B158DRAFT_1146 - preQ(0) biosynthesis protein QueC, at 1,182,604 to 1,183,278
_1146
B158DRAFT_1147: B158DRAFT_1147 - hypothetical protein, at 1,183,352 to 1,183,792
_1147
Position (kb)
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1181.490 kb on - strand, within B158DRAFT_1143 at 1181.623 kb on - strand at 1181.697 kb on - strand at 1181.697 kb on - strand at 1181.697 kb on - strand at 1181.709 kb on - strand at 1181.711 kb on - strand at 1181.746 kb on + strand at 1181.755 kb on - strand at 1181.756 kb on - strand at 1181.756 kb on - strand at 1181.777 kb on + strand, within B158DRAFT_1144 at 1181.777 kb on + strand, within B158DRAFT_1144 at 1181.785 kb on - strand, within B158DRAFT_1144 at 1181.802 kb on - strand, within B158DRAFT_1144 at 1181.809 kb on - strand, within B158DRAFT_1144 at 1181.895 kb on + strand, within B158DRAFT_1144 at 1181.970 kb on + strand, within B158DRAFT_1144 at 1181.972 kb on + strand, within B158DRAFT_1144 at 1181.978 kb on - strand, within B158DRAFT_1144 at 1182.030 kb on + strand, within B158DRAFT_1144 at 1182.102 kb on + strand, within B158DRAFT_1144 at 1182.112 kb on + strand, within B158DRAFT_1144 at 1182.231 kb on + strand, within B158DRAFT_1144 at 1182.231 kb on + strand, within B158DRAFT_1144 at 1182.231 kb on + strand, within B158DRAFT_1144 at 1182.231 kb on + strand, within B158DRAFT_1144 at 1182.231 kb on + strand, within B158DRAFT_1144 at 1182.239 kb on - strand, within B158DRAFT_1144 at 1182.239 kb on - strand, within B158DRAFT_1144 at 1182.239 kb on - strand, within B158DRAFT_1144 at 1182.239 kb on - strand, within B158DRAFT_1144 at 1182.239 kb on - strand, within B158DRAFT_1144 at 1182.239 kb on - strand, within B158DRAFT_1144 at 1182.241 kb on + strand, within B158DRAFT_1144 at 1182.249 kb on + strand, within B158DRAFT_1144 at 1182.345 kb on + strand at 1182.372 kb on - strand at 1182.467 kb on + strand, within B158DRAFT_1145 at 1182.469 kb on + strand, within B158DRAFT_1145 at 1182.475 kb on - strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on + strand, within B158DRAFT_1145 at 1182.477 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.485 kb on - strand, within B158DRAFT_1145 at 1182.530 kb on + strand at 1182.530 kb on + strand at 1182.530 kb on + strand at 1182.530 kb on + strand at 1182.556 kb on + strand at 1182.556 kb on + strand at 1182.563 kb on + strand at 1182.564 kb on - strand at 1182.571 kb on - strand at 1182.622 kb on + strand at 1182.637 kb on + strand at 1182.647 kb on + strand at 1182.687 kb on - strand, within B158DRAFT_1146 at 1182.695 kb on - strand, within B158DRAFT_1146 at 1182.695 kb on - strand, within B158DRAFT_1146 at 1182.722 kb on + strand, within B158DRAFT_1146 at 1182.732 kb on + strand, within B158DRAFT_1146 at 1182.732 kb on + strand, within B158DRAFT_1146 at 1182.740 kb on - strand, within B158DRAFT_1146 at 1182.815 kb on - strand, within B158DRAFT_1146 at 1182.836 kb on + strand, within B158DRAFT_1146 at 1182.844 kb on - strand, within B158DRAFT_1146 at 1182.919 kb on + strand, within B158DRAFT_1146 at 1182.924 kb on + strand, within B158DRAFT_1146 at 1182.924 kb on + strand, within B158DRAFT_1146 at 1182.924 kb on + strand, within B158DRAFT_1146 at 1182.924 kb on + strand, within B158DRAFT_1146 at 1182.924 kb on + strand, within B158DRAFT_1146 at 1182.924 kb on + strand, within B158DRAFT_1146 at 1182.932 kb on - strand, within B158DRAFT_1146 at 1182.937 kb on + strand, within B158DRAFT_1146 at 1182.939 kb on + strand, within B158DRAFT_1146 at 1182.947 kb on - strand, within B158DRAFT_1146 at 1182.995 kb on - strand, within B158DRAFT_1146 at 1183.000 kb on - strand, within B158DRAFT_1146 at 1183.130 kb on + strand, within B158DRAFT_1146 at 1183.130 kb on + strand, within B158DRAFT_1146 at 1183.130 kb on + strand, within B158DRAFT_1146 at 1183.130 kb on + strand, within B158DRAFT_1146 at 1183.130 kb on + strand, within B158DRAFT_1146 at 1183.130 kb on + strand, within B158DRAFT_1146 at 1183.138 kb on - strand, within B158DRAFT_1146 at 1183.138 kb on - strand, within B158DRAFT_1146 at 1183.138 kb on - strand, within B158DRAFT_1146 at 1183.138 kb on - strand, within B158DRAFT_1146 at 1183.150 kb on - strand, within B158DRAFT_1146 at 1183.176 kb on + strand, within B158DRAFT_1146 at 1183.184 kb on - strand, within B158DRAFT_1146 at 1183.194 kb on + strand, within B158DRAFT_1146 at 1183.202 kb on - strand, within B158DRAFT_1146 at 1183.202 kb on - strand, within B158DRAFT_1146 at 1183.202 kb on - strand, within B158DRAFT_1146 at 1183.204 kb on - strand, within B158DRAFT_1146 at 1183.235 kb on + strand at 1183.235 kb on + strand at 1183.248 kb on + strand at 1183.256 kb on - strand at 1183.302 kb on + strand at 1183.331 kb on - strand at 1183.333 kb on - strand at 1183.335 kb on + strand at 1183.335 kb on + strand at 1183.335 kb on + strand at 1183.335 kb on + strand at 1183.335 kb on + strand at 1183.335 kb on + strand at 1183.335 kb on + strand at 1183.342 kb on + strand at 1183.343 kb on - strand at 1183.343 kb on - strand at 1183.350 kb on - strand at 1183.431 kb on + strand, within B158DRAFT_1147 at 1183.431 kb on + strand, within B158DRAFT_1147 at 1183.431 kb on + strand, within B158DRAFT_1147 at 1183.431 kb on + strand, within B158DRAFT_1147 at 1183.431 kb on - strand, within B158DRAFT_1147 at 1183.433 kb on + strand, within B158DRAFT_1147 at 1183.433 kb on + strand, within B158DRAFT_1147 at 1183.439 kb on - strand, within B158DRAFT_1147 at 1183.439 kb on - strand, within B158DRAFT_1147 at 1183.439 kb on - strand, within B158DRAFT_1147 at 1183.441 kb on - strand, within B158DRAFT_1147 at 1183.441 kb on - strand, within B158DRAFT_1147 at 1183.441 kb on - strand, within B158DRAFT_1147 at 1183.442 kb on + strand, within B158DRAFT_1147 at 1183.442 kb on + strand, within B158DRAFT_1147 at 1183.442 kb on + strand, within B158DRAFT_1147 at 1183.464 kb on + strand, within B158DRAFT_1147 at 1183.464 kb on + strand, within B158DRAFT_1147 at 1183.464 kb on + strand, within B158DRAFT_1147 at 1183.472 kb on - strand, within B158DRAFT_1147 at 1183.472 kb on - strand, within B158DRAFT_1147 at 1183.472 kb on - strand, within B158DRAFT_1147 at 1183.472 kb on - strand, within B158DRAFT_1147 at 1183.472 kb on - strand, within B158DRAFT_1147 at 1183.472 kb on - strand, within B158DRAFT_1147 at 1183.485 kb on + strand, within B158DRAFT_1147
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 1,181,490 - B158DRAFT_1143 0.85 +1.4 1,181,623 - +0.2 1,181,697 - -0.5 1,181,697 - -0.2 1,181,697 - -0.3 1,181,709 - -0.1 1,181,711 - +0.3 1,181,746 + -1.0 1,181,755 - -0.1 1,181,756 - +2.5 1,181,756 - -0.1 1,181,777 + B158DRAFT_1144 0.11 -0.6 1,181,777 + B158DRAFT_1144 0.11 -0.2 1,181,785 - B158DRAFT_1144 0.12 +1.2 1,181,802 - B158DRAFT_1144 0.15 +0.7 1,181,809 - B158DRAFT_1144 0.16 +0.4 1,181,895 + B158DRAFT_1144 0.30 +0.0 1,181,970 + B158DRAFT_1144 0.41 +0.0 1,181,972 + B158DRAFT_1144 0.42 -0.2 1,181,978 - B158DRAFT_1144 0.43 +0.6 1,182,030 + B158DRAFT_1144 0.51 +0.3 1,182,102 + B158DRAFT_1144 0.62 +0.4 1,182,112 + B158DRAFT_1144 0.63 -0.0 1,182,231 + B158DRAFT_1144 0.82 -0.3 1,182,231 + B158DRAFT_1144 0.82 +0.1 1,182,231 + B158DRAFT_1144 0.82 +0.2 1,182,231 + B158DRAFT_1144 0.82 +0.1 1,182,231 + B158DRAFT_1144 0.82 +0.1 1,182,239 - B158DRAFT_1144 0.83 -0.5 1,182,239 - B158DRAFT_1144 0.83 +0.2 1,182,239 - B158DRAFT_1144 0.83 -0.2 1,182,239 - B158DRAFT_1144 0.83 -0.2 1,182,239 - B158DRAFT_1144 0.83 -0.3 1,182,239 - B158DRAFT_1144 0.83 +0.1 1,182,241 + B158DRAFT_1144 0.83 +0.7 1,182,249 + B158DRAFT_1144 0.85 -0.0 1,182,345 + -0.2 1,182,372 - -0.3 1,182,467 + B158DRAFT_1145 0.59 +0.2 1,182,469 + B158DRAFT_1145 0.61 -0.3 1,182,475 - B158DRAFT_1145 0.67 +0.3 1,182,477 + B158DRAFT_1145 0.69 +0.3 1,182,477 + B158DRAFT_1145 0.69 +0.6 1,182,477 + B158DRAFT_1145 0.69 -0.2 1,182,477 + B158DRAFT_1145 0.69 -0.3 1,182,477 + B158DRAFT_1145 0.69 -0.1 1,182,477 + B158DRAFT_1145 0.69 -0.6 1,182,477 + B158DRAFT_1145 0.69 -0.1 1,182,477 + B158DRAFT_1145 0.69 -1.5 1,182,477 + B158DRAFT_1145 0.69 -0.4 1,182,477 + B158DRAFT_1145 0.69 +0.1 1,182,477 + B158DRAFT_1145 0.69 -0.2 1,182,477 + B158DRAFT_1145 0.69 -0.1 1,182,477 - B158DRAFT_1145 0.69 -0.0 1,182,485 - B158DRAFT_1145 0.76 -0.9 1,182,485 - B158DRAFT_1145 0.76 -0.4 1,182,485 - B158DRAFT_1145 0.76 +0.8 1,182,485 - B158DRAFT_1145 0.76 +0.2 1,182,485 - B158DRAFT_1145 0.76 -0.0 1,182,485 - B158DRAFT_1145 0.76 -0.4 1,182,485 - B158DRAFT_1145 0.76 -0.1 1,182,485 - B158DRAFT_1145 0.76 -0.2 1,182,485 - B158DRAFT_1145 0.76 -0.1 1,182,530 + +0.3 1,182,530 + -0.2 1,182,530 + -0.6 1,182,530 + -0.9 1,182,556 + +1.1 1,182,556 + -0.6 1,182,563 + -0.3 1,182,564 - -2.3 1,182,571 - +0.2 1,182,622 + -0.5 1,182,637 + +0.5 1,182,647 + -0.1 1,182,687 - B158DRAFT_1146 0.12 +0.5 1,182,695 - B158DRAFT_1146 0.13 -0.3 1,182,695 - B158DRAFT_1146 0.13 -0.3 1,182,722 + B158DRAFT_1146 0.17 +0.1 1,182,732 + B158DRAFT_1146 0.19 +0.1 1,182,732 + B158DRAFT_1146 0.19 +0.4 1,182,740 - B158DRAFT_1146 0.20 +0.1 1,182,815 - B158DRAFT_1146 0.31 -0.4 1,182,836 + B158DRAFT_1146 0.34 +0.2 1,182,844 - B158DRAFT_1146 0.36 -0.1 1,182,919 + B158DRAFT_1146 0.47 -0.1 1,182,924 + B158DRAFT_1146 0.47 +0.3 1,182,924 + B158DRAFT_1146 0.47 -0.0 1,182,924 + B158DRAFT_1146 0.47 +0.1 1,182,924 + B158DRAFT_1146 0.47 +0.6 1,182,924 + B158DRAFT_1146 0.47 +0.0 1,182,924 + B158DRAFT_1146 0.47 -0.1 1,182,932 - B158DRAFT_1146 0.49 -0.3 1,182,937 + B158DRAFT_1146 0.49 -0.2 1,182,939 + B158DRAFT_1146 0.50 -0.3 1,182,947 - B158DRAFT_1146 0.51 -0.0 1,182,995 - B158DRAFT_1146 0.58 +0.1 1,183,000 - B158DRAFT_1146 0.59 -0.1 1,183,130 + B158DRAFT_1146 0.78 -0.1 1,183,130 + B158DRAFT_1146 0.78 -0.0 1,183,130 + B158DRAFT_1146 0.78 +0.3 1,183,130 + B158DRAFT_1146 0.78 +0.7 1,183,130 + B158DRAFT_1146 0.78 -0.0 1,183,130 + B158DRAFT_1146 0.78 +0.3 1,183,138 - B158DRAFT_1146 0.79 -0.0 1,183,138 - B158DRAFT_1146 0.79 +0.4 1,183,138 - B158DRAFT_1146 0.79 +0.1 1,183,138 - B158DRAFT_1146 0.79 +0.3 1,183,150 - B158DRAFT_1146 0.81 +0.2 1,183,176 + B158DRAFT_1146 0.85 -0.5 1,183,184 - B158DRAFT_1146 0.86 -0.9 1,183,194 + B158DRAFT_1146 0.87 -0.0 1,183,202 - B158DRAFT_1146 0.89 -0.5 1,183,202 - B158DRAFT_1146 0.89 -0.5 1,183,202 - B158DRAFT_1146 0.89 -0.9 1,183,204 - B158DRAFT_1146 0.89 +1.0 1,183,235 + +0.5 1,183,235 + -0.1 1,183,248 + +0.1 1,183,256 - +1.3 1,183,302 + -0.4 1,183,331 - -0.4 1,183,333 - -0.3 1,183,335 + -0.3 1,183,335 + -0.1 1,183,335 + -0.7 1,183,335 + -0.2 1,183,335 + +0.4 1,183,335 + -0.1 1,183,335 + +0.5 1,183,342 + -2.2 1,183,343 - -0.4 1,183,343 - +0.0 1,183,350 - -0.0 1,183,431 + B158DRAFT_1147 0.18 -0.2 1,183,431 + B158DRAFT_1147 0.18 -0.9 1,183,431 + B158DRAFT_1147 0.18 -1.5 1,183,431 + B158DRAFT_1147 0.18 +0.1 1,183,431 - B158DRAFT_1147 0.18 -0.2 1,183,433 + B158DRAFT_1147 0.18 +0.2 1,183,433 + B158DRAFT_1147 0.18 -0.3 1,183,439 - B158DRAFT_1147 0.20 -0.2 1,183,439 - B158DRAFT_1147 0.20 -0.2 1,183,439 - B158DRAFT_1147 0.20 +0.1 1,183,441 - B158DRAFT_1147 0.20 -0.1 1,183,441 - B158DRAFT_1147 0.20 +0.5 1,183,441 - B158DRAFT_1147 0.20 -0.2 1,183,442 + B158DRAFT_1147 0.20 +0.7 1,183,442 + B158DRAFT_1147 0.20 -0.3 1,183,442 + B158DRAFT_1147 0.20 -0.2 1,183,464 + B158DRAFT_1147 0.25 -0.5 1,183,464 + B158DRAFT_1147 0.25 -1.9 1,183,464 + B158DRAFT_1147 0.25 -0.9 1,183,472 - B158DRAFT_1147 0.27 -0.0 1,183,472 - B158DRAFT_1147 0.27 -0.1 1,183,472 - B158DRAFT_1147 0.27 +0.4 1,183,472 - B158DRAFT_1147 0.27 +0.4 1,183,472 - B158DRAFT_1147 0.27 -0.6 1,183,472 - B158DRAFT_1147 0.27 -0.1 1,183,485 + B158DRAFT_1147 0.30 +0.0
Or see this region's nucleotide sequence