Experiment: Kang_ML4_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0415 and B158DRAFT_0416 are separated by 173 nucleotides B158DRAFT_0416 and B158DRAFT_0417 are separated by 75 nucleotides B158DRAFT_0417 and B158DRAFT_0418 are separated by 11 nucleotides B158DRAFT_0418 and B158DRAFT_0419 are separated by 20 nucleotides
B158DRAFT_0415: B158DRAFT_0415 - transcription termination factor Rho, at 418,269 to 419,525
_0415
B158DRAFT_0416: B158DRAFT_0416 - hypothetical protein, at 419,699 to 420,301
_0416
B158DRAFT_0417: B158DRAFT_0417 - UbiD family decarboxylases, at 420,377 to 421,864
_0417
B158DRAFT_0418: B158DRAFT_0418 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 421,876 to 422,625
_0418
B158DRAFT_0419: B158DRAFT_0419 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 422,646 to 423,863
_0419
Position (kb)
420
421
422 Strain fitness (log2 ratio)
-1
0
1
2
3 at 419.575 kb on + strand at 419.577 kb on + strand at 419.610 kb on + strand at 419.616 kb on + strand at 419.678 kb on + strand at 419.679 kb on + strand at 419.679 kb on + strand at 419.687 kb on - strand at 419.721 kb on + strand at 419.742 kb on - strand at 419.814 kb on + strand, within B158DRAFT_0416 at 419.888 kb on + strand, within B158DRAFT_0416 at 420.017 kb on - strand, within B158DRAFT_0416 at 420.031 kb on + strand, within B158DRAFT_0416 at 420.039 kb on - strand, within B158DRAFT_0416 at 420.073 kb on - strand, within B158DRAFT_0416 at 420.132 kb on - strand, within B158DRAFT_0416 at 420.153 kb on - strand, within B158DRAFT_0416 at 420.182 kb on + strand, within B158DRAFT_0416 at 420.190 kb on - strand, within B158DRAFT_0416 at 420.212 kb on - strand, within B158DRAFT_0416 at 420.212 kb on - strand, within B158DRAFT_0416 at 420.236 kb on + strand, within B158DRAFT_0416 at 420.237 kb on + strand, within B158DRAFT_0416 at 420.314 kb on + strand at 421.840 kb on - strand at 421.840 kb on - strand at 421.865 kb on - strand at 421.865 kb on - strand at 421.962 kb on - strand, within B158DRAFT_0418 at 421.966 kb on + strand, within B158DRAFT_0418 at 421.996 kb on + strand, within B158DRAFT_0418 at 422.008 kb on - strand, within B158DRAFT_0418 at 422.069 kb on + strand, within B158DRAFT_0418 at 422.074 kb on + strand, within B158DRAFT_0418 at 422.074 kb on + strand, within B158DRAFT_0418 at 422.090 kb on - strand, within B158DRAFT_0418 at 422.105 kb on - strand, within B158DRAFT_0418 at 422.116 kb on + strand, within B158DRAFT_0418 at 422.163 kb on - strand, within B158DRAFT_0418 at 422.164 kb on - strand, within B158DRAFT_0418 at 422.171 kb on - strand, within B158DRAFT_0418 at 422.174 kb on - strand, within B158DRAFT_0418 at 422.219 kb on - strand, within B158DRAFT_0418 at 422.254 kb on - strand, within B158DRAFT_0418 at 422.313 kb on + strand, within B158DRAFT_0418 at 422.313 kb on + strand, within B158DRAFT_0418 at 422.321 kb on - strand, within B158DRAFT_0418 at 422.321 kb on - strand, within B158DRAFT_0418 at 422.331 kb on - strand, within B158DRAFT_0418 at 422.348 kb on + strand, within B158DRAFT_0418 at 422.348 kb on + strand, within B158DRAFT_0418 at 422.356 kb on - strand, within B158DRAFT_0418 at 422.359 kb on + strand, within B158DRAFT_0418 at 422.359 kb on + strand, within B158DRAFT_0418 at 422.359 kb on + strand, within B158DRAFT_0418 at 422.367 kb on - strand, within B158DRAFT_0418 at 422.367 kb on - strand, within B158DRAFT_0418 at 422.367 kb on - strand, within B158DRAFT_0418 at 422.367 kb on - strand, within B158DRAFT_0418 at 422.367 kb on - strand, within B158DRAFT_0418 at 422.367 kb on - strand, within B158DRAFT_0418 at 422.369 kb on + strand, within B158DRAFT_0418 at 422.377 kb on - strand, within B158DRAFT_0418 at 422.435 kb on + strand, within B158DRAFT_0418 at 422.444 kb on - strand, within B158DRAFT_0418 at 422.444 kb on - strand, within B158DRAFT_0418 at 422.451 kb on - strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.510 kb on + strand, within B158DRAFT_0418 at 422.518 kb on - strand, within B158DRAFT_0418 at 422.518 kb on - strand, within B158DRAFT_0418 at 422.518 kb on - strand, within B158DRAFT_0418 at 422.518 kb on - strand, within B158DRAFT_0418 at 422.619 kb on + strand at 422.619 kb on + strand at 422.627 kb on - strand at 422.627 kb on - strand at 422.637 kb on - strand at 422.671 kb on + strand at 422.720 kb on + strand at 422.725 kb on + strand at 422.725 kb on + strand at 422.725 kb on + strand at 422.733 kb on - strand at 422.733 kb on - strand at 422.758 kb on + strand at 422.783 kb on + strand, within B158DRAFT_0419 at 422.783 kb on + strand, within B158DRAFT_0419 at 422.791 kb on - strand, within B158DRAFT_0419 at 422.791 kb on - strand, within B158DRAFT_0419 at 422.855 kb on + strand, within B158DRAFT_0419 at 422.855 kb on + strand, within B158DRAFT_0419 at 422.855 kb on + strand, within B158DRAFT_0419 at 422.855 kb on + strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419 at 422.863 kb on - strand, within B158DRAFT_0419
Per-strain Table
Position Strand Gene LocusTag Fraction Kang_ML4_MIT diversity barseq remove 419,575 + -0.2 419,577 + -0.0 419,610 + -0.6 419,616 + -0.2 419,678 + -1.0 419,679 + +0.4 419,679 + -0.4 419,687 - +0.7 419,721 + -0.8 419,742 - +0.6 419,814 + B158DRAFT_0416 0.19 +0.3 419,888 + B158DRAFT_0416 0.31 -0.1 420,017 - B158DRAFT_0416 0.53 -0.8 420,031 + B158DRAFT_0416 0.55 +0.8 420,039 - B158DRAFT_0416 0.56 -0.3 420,073 - B158DRAFT_0416 0.62 -0.2 420,132 - B158DRAFT_0416 0.72 -1.5 420,153 - B158DRAFT_0416 0.75 -0.3 420,182 + B158DRAFT_0416 0.80 +1.1 420,190 - B158DRAFT_0416 0.81 -0.1 420,212 - B158DRAFT_0416 0.85 +0.5 420,212 - B158DRAFT_0416 0.85 -0.0 420,236 + B158DRAFT_0416 0.89 -0.6 420,237 + B158DRAFT_0416 0.89 -0.2 420,314 + +0.1 421,840 - -0.0 421,840 - +0.7 421,865 - +1.0 421,865 - +1.0 421,962 - B158DRAFT_0418 0.11 +1.6 421,966 + B158DRAFT_0418 0.12 +1.5 421,996 + B158DRAFT_0418 0.16 +3.0 422,008 - B158DRAFT_0418 0.18 +3.0 422,069 + B158DRAFT_0418 0.26 +1.8 422,074 + B158DRAFT_0418 0.26 +2.3 422,074 + B158DRAFT_0418 0.26 +2.2 422,090 - B158DRAFT_0418 0.29 +1.2 422,105 - B158DRAFT_0418 0.31 -0.3 422,116 + B158DRAFT_0418 0.32 +1.2 422,163 - B158DRAFT_0418 0.38 +2.6 422,164 - B158DRAFT_0418 0.38 +2.2 422,171 - B158DRAFT_0418 0.39 -0.0 422,174 - B158DRAFT_0418 0.40 +0.4 422,219 - B158DRAFT_0418 0.46 +1.9 422,254 - B158DRAFT_0418 0.50 +3.0 422,313 + B158DRAFT_0418 0.58 +2.0 422,313 + B158DRAFT_0418 0.58 +1.4 422,321 - B158DRAFT_0418 0.59 -0.0 422,321 - B158DRAFT_0418 0.59 +1.0 422,331 - B158DRAFT_0418 0.61 +1.2 422,348 + B158DRAFT_0418 0.63 +2.9 422,348 + B158DRAFT_0418 0.63 +1.2 422,356 - B158DRAFT_0418 0.64 +2.0 422,359 + B158DRAFT_0418 0.64 +1.7 422,359 + B158DRAFT_0418 0.64 +2.7 422,359 + B158DRAFT_0418 0.64 +1.0 422,367 - B158DRAFT_0418 0.65 +1.8 422,367 - B158DRAFT_0418 0.65 +2.0 422,367 - B158DRAFT_0418 0.65 +1.6 422,367 - B158DRAFT_0418 0.65 +2.6 422,367 - B158DRAFT_0418 0.65 -0.6 422,367 - B158DRAFT_0418 0.65 +1.4 422,369 + B158DRAFT_0418 0.66 +0.8 422,377 - B158DRAFT_0418 0.67 +0.6 422,435 + B158DRAFT_0418 0.75 +2.0 422,444 - B158DRAFT_0418 0.76 +3.0 422,444 - B158DRAFT_0418 0.76 +0.6 422,451 - B158DRAFT_0418 0.77 +1.6 422,510 + B158DRAFT_0418 0.85 +3.3 422,510 + B158DRAFT_0418 0.85 +0.3 422,510 + B158DRAFT_0418 0.85 +1.6 422,510 + B158DRAFT_0418 0.85 +2.0 422,510 + B158DRAFT_0418 0.85 +1.0 422,510 + B158DRAFT_0418 0.85 +1.0 422,510 + B158DRAFT_0418 0.85 +2.0 422,510 + B158DRAFT_0418 0.85 +2.0 422,518 - B158DRAFT_0418 0.86 -1.0 422,518 - B158DRAFT_0418 0.86 +1.0 422,518 - B158DRAFT_0418 0.86 +1.3 422,518 - B158DRAFT_0418 0.86 +2.3 422,619 + +0.2 422,619 + -0.3 422,627 - +0.1 422,627 - +0.1 422,637 - -0.4 422,671 + -1.1 422,720 + +0.0 422,725 + +0.1 422,725 + -0.9 422,725 + -0.9 422,733 - -0.0 422,733 - -0.1 422,758 + -0.6 422,783 + B158DRAFT_0419 0.11 -0.1 422,783 + B158DRAFT_0419 0.11 +0.4 422,791 - B158DRAFT_0419 0.12 -0.2 422,791 - B158DRAFT_0419 0.12 -0.0 422,855 + B158DRAFT_0419 0.17 -0.6 422,855 + B158DRAFT_0419 0.17 -0.0 422,855 + B158DRAFT_0419 0.17 -1.0 422,855 + B158DRAFT_0419 0.17 +0.3 422,863 - B158DRAFT_0419 0.18 +0.3 422,863 - B158DRAFT_0419 0.18 +0.1 422,863 - B158DRAFT_0419 0.18 -0.7 422,863 - B158DRAFT_0419 0.18 -0.1 422,863 - B158DRAFT_0419 0.18 -0.1 422,863 - B158DRAFT_0419 0.18 +0.1 422,863 - B158DRAFT_0419 0.18 -0.2
Or see this region's nucleotide sequence