Experiment: R2A with G418 disolution 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5027 and Atu5028 overlap by 4 nucleotides Atu5028 and Atu5029 are separated by 170 nucleotides Atu5029 and Atu5030 are separated by 160 nucleotides Atu5030 and Atu5031 are separated by 60 nucleotides
Atu5027: Atu5027 - IS 426 transposase, at 28,825 to 29,379
Atu5027
Atu5028: Atu5028 - IS426 transposase, at 29,376 to 29,753
Atu5028
Atu5029: Atu5029 - hypothetical protein, at 29,924 to 30,694
Atu5029
Atu5030: Atu5030 - hypothetical protein, at 30,855 to 31,397
Atu5030
Atu5031: Atu5031 - hypothetical protein, at 31,458 to 32,576
Atu5031
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 28.999 kb on - strand, within Atu5027 at 29.022 kb on + strand, within Atu5027 at 29.023 kb on + strand, within Atu5027 at 29.030 kb on - strand, within Atu5027 at 29.044 kb on + strand, within Atu5027 at 29.044 kb on + strand, within Atu5027 at 29.052 kb on - strand, within Atu5027 at 29.052 kb on - strand, within Atu5027 at 29.056 kb on + strand, within Atu5027 at 29.063 kb on + strand, within Atu5027 at 29.063 kb on + strand, within Atu5027 at 29.071 kb on - strand, within Atu5027 at 29.071 kb on - strand, within Atu5027 at 29.078 kb on - strand, within Atu5027 at 29.105 kb on + strand, within Atu5027 at 29.108 kb on - strand, within Atu5027 at 29.110 kb on - strand, within Atu5027 at 29.124 kb on - strand, within Atu5027 at 29.166 kb on + strand, within Atu5027 at 29.167 kb on - strand, within Atu5027 at 29.172 kb on - strand, within Atu5027 at 29.180 kb on - strand, within Atu5027 at 29.180 kb on - strand, within Atu5027 at 29.190 kb on - strand, within Atu5027 at 29.203 kb on - strand, within Atu5027 at 29.203 kb on - strand, within Atu5027 at 29.205 kb on - strand, within Atu5027 at 29.360 kb on - strand at 29.441 kb on - strand, within Atu5028 at 29.629 kb on + strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.697 kb on - strand, within Atu5028 at 29.750 kb on + strand at 29.756 kb on + strand at 29.762 kb on + strand at 29.762 kb on + strand at 29.762 kb on + strand at 29.762 kb on + strand at 29.764 kb on - strand at 29.770 kb on - strand at 29.770 kb on - strand at 29.780 kb on - strand at 29.846 kb on - strand at 29.923 kb on - strand at 29.938 kb on + strand at 29.983 kb on - strand at 30.003 kb on - strand, within Atu5029 at 30.074 kb on + strand, within Atu5029 at 30.089 kb on - strand, within Atu5029 at 30.089 kb on - strand, within Atu5029 at 30.094 kb on - strand, within Atu5029 at 30.128 kb on + strand, within Atu5029 at 30.183 kb on - strand, within Atu5029 at 30.206 kb on - strand, within Atu5029 at 30.208 kb on + strand, within Atu5029 at 30.268 kb on + strand, within Atu5029 at 30.276 kb on - strand, within Atu5029 at 30.276 kb on - strand, within Atu5029 at 30.294 kb on + strand, within Atu5029 at 30.321 kb on - strand, within Atu5029 at 30.338 kb on - strand, within Atu5029 at 30.347 kb on - strand, within Atu5029 at 30.416 kb on + strand, within Atu5029 at 30.562 kb on - strand, within Atu5029 at 30.581 kb on + strand, within Atu5029 at 30.644 kb on - strand at 30.706 kb on - strand at 30.706 kb on - strand at 30.725 kb on + strand at 30.760 kb on - strand at 30.762 kb on + strand at 30.853 kb on + strand at 31.006 kb on + strand, within Atu5030 at 31.006 kb on + strand, within Atu5030 at 31.006 kb on + strand, within Atu5030 at 31.016 kb on + strand, within Atu5030 at 31.016 kb on + strand, within Atu5030 at 31.019 kb on - strand, within Atu5030 at 31.027 kb on + strand, within Atu5030 at 31.050 kb on + strand, within Atu5030 at 31.064 kb on + strand, within Atu5030 at 31.076 kb on + strand, within Atu5030 at 31.084 kb on - strand, within Atu5030 at 31.132 kb on + strand, within Atu5030 at 31.133 kb on - strand, within Atu5030 at 31.191 kb on - strand, within Atu5030 at 31.193 kb on + strand, within Atu5030 at 31.201 kb on - strand, within Atu5030 at 31.214 kb on - strand, within Atu5030 at 31.233 kb on - strand, within Atu5030 at 31.238 kb on - strand, within Atu5030 at 31.266 kb on + strand, within Atu5030 at 31.321 kb on + strand, within Atu5030 at 31.356 kb on + strand at 31.371 kb on + strand at 31.402 kb on - strand at 31.617 kb on + strand, within Atu5031 at 31.624 kb on - strand, within Atu5031 at 31.632 kb on - strand, within Atu5031 at 31.632 kb on - strand, within Atu5031 at 31.646 kb on - strand, within Atu5031
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with G418 disolution 0.02 mM remove 28,999 - Atu5027 0.31 -0.9 29,022 + Atu5027 0.35 -0.8 29,023 + Atu5027 0.36 -0.0 29,030 - Atu5027 0.37 +1.2 29,044 + Atu5027 0.39 -0.1 29,044 + Atu5027 0.39 -0.4 29,052 - Atu5027 0.41 +1.1 29,052 - Atu5027 0.41 +1.6 29,056 + Atu5027 0.42 -1.0 29,063 + Atu5027 0.43 -0.3 29,063 + Atu5027 0.43 -1.1 29,071 - Atu5027 0.44 +0.1 29,071 - Atu5027 0.44 +0.2 29,078 - Atu5027 0.46 -1.5 29,105 + Atu5027 0.50 +0.5 29,108 - Atu5027 0.51 +0.7 29,110 - Atu5027 0.51 +1.5 29,124 - Atu5027 0.54 +0.0 29,166 + Atu5027 0.61 +0.9 29,167 - Atu5027 0.62 -0.1 29,172 - Atu5027 0.63 +2.2 29,180 - Atu5027 0.64 +1.2 29,180 - Atu5027 0.64 +0.9 29,190 - Atu5027 0.66 +0.4 29,203 - Atu5027 0.68 +1.4 29,203 - Atu5027 0.68 +0.3 29,205 - Atu5027 0.68 +0.7 29,360 - +0.7 29,441 - Atu5028 0.17 -0.4 29,629 + Atu5028 0.67 +0.8 29,697 - Atu5028 0.85 +0.4 29,697 - Atu5028 0.85 -3.0 29,697 - Atu5028 0.85 +0.4 29,697 - Atu5028 0.85 +1.2 29,750 + -0.4 29,756 + -0.0 29,762 + +0.7 29,762 + +1.0 29,762 + +0.4 29,762 + +0.2 29,764 - -0.0 29,770 - +0.1 29,770 - +0.7 29,780 - -0.5 29,846 - -1.1 29,923 - -0.1 29,938 + -0.0 29,983 - -0.9 30,003 - Atu5029 0.10 +0.1 30,074 + Atu5029 0.19 -1.7 30,089 - Atu5029 0.21 -0.0 30,089 - Atu5029 0.21 -0.6 30,094 - Atu5029 0.22 -0.6 30,128 + Atu5029 0.26 +1.3 30,183 - Atu5029 0.34 -1.0 30,206 - Atu5029 0.37 +0.0 30,208 + Atu5029 0.37 -0.8 30,268 + Atu5029 0.45 -0.6 30,276 - Atu5029 0.46 -0.3 30,276 - Atu5029 0.46 -0.2 30,294 + Atu5029 0.48 -0.0 30,321 - Atu5029 0.51 -0.8 30,338 - Atu5029 0.54 +0.6 30,347 - Atu5029 0.55 +1.8 30,416 + Atu5029 0.64 -0.3 30,562 - Atu5029 0.83 -1.3 30,581 + Atu5029 0.85 -0.8 30,644 - -1.3 30,706 - -0.2 30,706 - -0.8 30,725 + +1.2 30,760 - -0.5 30,762 + +0.4 30,853 + -1.8 31,006 + Atu5030 0.28 -1.3 31,006 + Atu5030 0.28 -0.7 31,006 + Atu5030 0.28 +0.2 31,016 + Atu5030 0.30 +0.4 31,016 + Atu5030 0.30 +0.3 31,019 - Atu5030 0.30 -0.0 31,027 + Atu5030 0.32 -1.2 31,050 + Atu5030 0.36 +0.2 31,064 + Atu5030 0.38 +0.0 31,076 + Atu5030 0.41 +0.8 31,084 - Atu5030 0.42 +0.3 31,132 + Atu5030 0.51 +0.4 31,133 - Atu5030 0.51 +0.4 31,191 - Atu5030 0.62 +0.0 31,193 + Atu5030 0.62 +0.7 31,201 - Atu5030 0.64 +0.0 31,214 - Atu5030 0.66 -1.1 31,233 - Atu5030 0.70 -0.4 31,238 - Atu5030 0.71 +2.0 31,266 + Atu5030 0.76 -0.5 31,321 + Atu5030 0.86 -0.4 31,356 + -0.3 31,371 + -1.3 31,402 - +0.1 31,617 + Atu5031 0.14 +0.7 31,624 - Atu5031 0.15 +0.5 31,632 - Atu5031 0.16 +0.3 31,632 - Atu5031 0.16 -1.3 31,646 - Atu5031 0.17 -0.6
Or see this region's nucleotide sequence